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Journal of Tea Science ›› 2024, Vol. 44 ›› Issue (2): 193-206.doi: 10.13305/j.cnki.jts.2024.02.002

• Research Paper • Previous Articles     Next Articles

Genetic Diversity and Population Structure Relationship Analysis of Wild Tea Germplasm Resources in Badong County, Hubei Province

CUI Qingmei1, LIANG Jinbo1, MA Huijie1, HU Shuangling1, CHEN Qinghua1, WU Liyun2, HE Mengdi2, WANG Liubin2, TAN Licai3, ZHANG Qiang1,*, WANG Liyuan2,*   

  1. 1. Enshi Tujia and Miao Autonomous Prefecture Academy of Agricultural Sciences, Enshi 445000, China;
    2. Tea Research Institute of Chinese Academy of Agricultural Sciences, Hangzhou 310008, China;
    3. Hubei Zhengshantang Badong Black Tea Co., Ltd., Hubei Province, Badong 444300, China
  • Received:2023-09-23 Revised:2023-12-11 Online:2024-04-15 Published:2024-04-30

Abstract: Wild tea germplasm resources have high genetic diversity and are also a high-quality source for the breeding and utilization of local tea cultivars. In this study, 26 resources of wild tea plants from Badong County, Hubei Province, were collected and the genetic diversity and population structure of wild tea plants were analyzed using SSR molecular markers with the normal tea cultivars as the control. The results are as follows: (1) 16 pairs of primers detected an average of 5.12 alleles and 3.65 effective loci in the test materials. A total of 82 alleles were amplified, with each pair of primers amplifying a range of 3-8 labeled alleles. The average Shannon diversity index was 1.378. (2) Six core primer sites were selected from 16 pairs of primers, which can effectively detect and identify 26 materials in this study. (3) UPGMA evolutionary map of individual samples could divide all 48 materials into 7 categories. Wild tea plants and cultivated tea plants could be effectively divided through SSR detection. Population genetic structure analysis suggests that 26 wild tea samples could be divided into 2 subgroups. (4) Based on biochemical components, two samples with high EGCG content and two tea germplasms suitable for making black tea were selected. Results of this research show that diversity level of wild tea resources in Badong was high, with high genetic variation within the population. This study laid a foundation for further protection, development and utilization of wild tea germplasm resources in Badong.

Key words: wild tea plant, diversity, SSR, genetic relationship

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