Diversity Analysis of Leaf Phenotype and Biochemical Components in Tea Local Population Resources

TANG Lu; LI Changle; GE Yue; WANG Pu; ZHAO Hua; WANG Mingle; WANG Yu; GUO Fei; NI Dejiang

doi:10.13305/j.cnki.jts.2023.04.008

PDF(528 KB)

Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (4) : 473-488. DOI: 10.13305/j.cnki.jts.2023.04.008

Research Paper

Diversity Analysis of Leaf Phenotype and Biochemical Components in Tea Local Population Resources

TANG Lu, LI Changle, GE Yue, WANG Pu, ZHAO Hua, WANG Mingle, WANG Yu, GUO Fei^*, NI Dejiang

Author information +

History +

Abstract

In order to protect tea germplasm resources and promote tea germplasm innovation, the phenotypic character and biochemical component diversity of 32 tea germplasm resources from 12 provinces were studied. The results showed that the genetic diversity index of 16 phenotypic character ranged from 0.81 to 1.94, and the phenotypic genetic diversity of the test population was high. The cumulative contribution rate of the six phenotypic characters including leaf area, leaf shape, leaf upper surface, young shoot pubescence, leaf tip shape and leaf cross section were 71.78%, which was the main factor causing the phenotypic difference of the tested tea population. Based on the determination results of biochemical components in two years, the variation coefficient of caffeine content (17.95% and 14.55%) was the largest, followed by the variation coefficient of tea polyphenol content (13.61% and 8.11%). The variation coefficient of free amino acid (5.62% and 7.52%) was the lowest. Cluster analysis based on the results of biochemical component content determination was conducted to divide the tested tea tree populations into three groups, each including different types of germplasm. From the geographical distribution of the tested tea populations, the content of biochemical components showed regional differences.

Key words

biochemical components / diversity / phenotypic character / tea local population species

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

TANG Lu, LI Changle, GE Yue, WANG Pu, ZHAO Hua, WANG Mingle, WANG Yu, GUO Fei, NI Dejiang. Diversity Analysis of Leaf Phenotype and Biochemical Components in Tea Local Population Resources[J]. Journal of Tea Science. 2023, 43(4): 473-488 https://doi.org/10.13305/j.cnki.jts.2023.04.008

References

[1] 郭燕, 张树航, 李颖, 等. 燕山板栗种质资源叶片表型性状多样性研究[J]. 园艺学报, 2022, 49(8): 1673-1688.
Guo Y, Zhang S H, Li Y, et al.Diversity analysis of leaves phenotypic traits of Yanshan Chestnut[J]. Acta Horticulturae Sinica, 2022, 49(8): 1673-1688.
[2] 姬广磊. 黄鮟鱇(Lophius litulon)形态特征及其遗传学研究[D]. 青岛: 中国海洋大学, 2007.
Ji G L.Study on the morphological characters and genetics of Lophius litulon [D]. Qingdao: Ocean University of China, 2007.
[3] 陈琪予, 马建强, 陈杰丹, 等. 利用图像特征分析茶树成熟叶表型的遗传多样性[J]. 茶叶科学, 2022, 42(5): 649-660.
Chen Q Y, Ma J Q, Chen J D, et al.Genetic diversity of mature leaves of tea germplasms based on image features[J]. Journal of Tea Science, 2022, 42(5): 649-660.
[4] Naegele R P, Mitchell J, Hausbeck M K.Genetic diversity, population structure, and heritability of fruit traits in Capsicum annuum[J]. PLos One, 2016, 11(7): e0156969. doi: 10.1371/journal.pone.0156969
[5] 吴国林. 基于ISSR和SSR标记的鄱阳湖流域野生菰种质资源遗传多样性研究[D]. 南昌: 江西农业大学, 2014.
Wu G L.Studies on genetic diversity of germplasm resources of Zizania latifolia (Griseb.) Trucz. based on ISSR and SSR markers [D]. Nanchang: Jiangxi Agricultural University, 2014.
[6] 刘彤. 柳州九万山野生茶树种质资源研究[D]. 长沙: 湖南农业大学, 2016.
Liu T.The research of Liuzhou Jiuwan Mountain wild tea germplasm resources [D]. Changsha: Hunan Agricultural University, 2016.
[7] 莫岚, 黄亚辉, 赵文芳, 等. 金秀野生茶树群体的生化特征[J]. 福建农林大学学报(自然科学版), 2018, 47(4): 409-414.
Mo L, Huang Y H, Zhao W F, et al.Analysis of biochemical components of wild tea colonies in Jinxiu[J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2018, 47(4): 409-414.
[8] 晏嫦妤, 李家贤, 黄华林, 等. 广东茶树群体品种品质成分差异初步研究[J]. 广东农业科学, 2009, 59(12): 39-41.
Yan C Y, Li J X, Huang H L, et al.Study on differences in quality composition of Guangdong tea germplasms[J]. Guangdong Agricultural Sciences, 2009, 59(12): 39-41.
[9] 黄亚辉, 罗荣康, 晏嫦妤, 等. 连南茶树种质资源叶片表型性状及生化成分的研究[J]. 广东茶业, 2017, 45(6): 11-13.
Huang Y H, Luo R K, Yan C Y, et al.Study on leaf phenotypic traits and biochemical components of tea germplasm resources in Liannan[J]. Guangdong Tea Industry, 2017, 45(6): 11-13.
[10] 王小萍, 唐晓波, 王迎春, 等. 52份茶树资源生化组分的表型多样性分析[J]. 茶叶科学, 2012, 32(2):129-134.
Wang X P, Tang X B, Wang Y C, et al.Phenotypic diversity analysis of biochemical composition in 52 tea germplasms[J]. Journal of Tea Science, 2012, 32(2): 129-134.
[11] 甘春萍, 邓慧群, 陈佳, 等. 广西地方群体品种主要生化成分分析研究[J]. 农业与技术, 2012, 32(4): 95-99.
Gan C P, Deng H Q, Chen J, et al.Analysis of main biochemical components of Guangxi local population varieties[J]. Agriculture and Technology, 2012, 32(4): 95-99.
[12] 万明轩. 菠菜表型三维点云数据分析与形态品质指标遗传多样性研究[D]. 上海: 上海师范大学, 2020.
Wan M X.Study on analysis of three-dimensional point cloud data of spinach phenotype and genetic diversity of morphological quality index [D]. Shanghai: Shanghai Normal University, 2020.
[13] 张悦, 索玉静, 孙鹏, 等. 柿种质资源果实形态多样性分析[J]. 园艺学报, 2022, 49(7): 1473-1490.
Zhang Y, Suo Y J, Sun P, et al.Analysis on fruit morphological diversity of persimmon germplasm resources[J]. Acta Horticulturae Sinica, 2022, 49(7): 1473-1490.
[14] 陈亮, 杨亚军. 茶树种质资源描述规范和数据标准[M]. 北京: 中国农业出版社, 2005.
Chen L, Yang Y J.Description specification and data standard of tea germplasm resources [M]. Beijing: China Agriculture Press, 2005.
[15] 郭荣华, 刘志斋, 蔡一林, 等. 两个历史上重要的玉米地方品种群体的表型多样性评价[J]. 玉米科学, 2009, 17(2): 24-28.
Guo R H, Liu Z Z, Cai Y L, et al.Evaluation of phenotypic diversity for two maize landrace population shaving historical importance in China[J]. Journal of Maize Sciences, 2009, 17(2): 24-28.
[16] 丁洲, 李烨昕, 袁艺, 等. 安徽茶区优良群体种的表型性状和遗传多样性分析[J]. 茶叶科学, 2018, 38(2):155-161.
Ding Z, Li Y X, Yuan Y, et al.Phenotypic traits and genetic diversity of elite tea population in Anhui (China)[J]. Journal of Tea Science, 2018, 38(2): 155-161.
[17] 黄海涛, 王风雷, 王贤波, 等. 鸠坑茶树资源芽叶表型性状的多样性分析[J]. 浙江农业科学, 2013, 64(3): 251-254.
Huang H T, Wang F L, Wang X B, et al.Diversity analysis of bud and leaf phenotypic traits of Jiukeng tea resources[J]. Journal of Zhejiang Agricultural Sciences, 2013, 64(3): 251-254.
[18] 刘青. 贵州三都县古茶树遗传多样性研究[D]. 贵阳: 贵州大学, 2020.
Liu Q.Genetic diversity of ancient tea trees in Sandu County, Guizhou Province [D]. Guiyang: Guizhou University, 2020.
[19] 周炎花. 基于叶片形态和EST-SSR茶树遗传多样性与遗传演化研究[D]. 福州: 福建农林大学, 2010.
Zhou Y H.Study of genetic diversity and genetic evolution of tea germplasms based on leaf morphology and EST-SSR markers [D]. Fuzhou: Fujian Agriculture and Forestry University, 2010.
[20] 胡波, 李国元, 谢志兵, 等. 主成分分析法在冬枣果实贮藏特性分析中的应用[J]. 食品科技, 2014, 39(2): 44-47.
Hu B, Li G Y, Xie Z B, et al.Application of principle component analysis on winter jujube storage characteristic[J]. Food Science and Technology, 2014, 39(2): 44-47.
[21] 杨玲, 张彩霞, 康国栋, 等. ‘华红’苹果果肉的流变特性及其主成分分析[J]. 中国农业科学, 2015, 48(12): 2417-2427.
Yang L, Zhang C X, Kang G D, et al.Rheologic properties of ‘Huahong’ apple pulp and their principal component analysis[J]. Scientia Agricultura Sinica, 2015, 48(12): 2417-2427.
[22] 郎彬彬, 黄春辉, 朱博, 等. 基于果实相关性状的江西野生毛花猕猴桃初级核心种质的构建方法研究[J]. 果树学报, 2016, 33(7): 794-803.
Lang B B, Huang C H, Zhu B, et al.Study on the method of constructing a primary core collection of Jiangxi wild Actinidia eriantha based on fruit traits[J]. Journal of Fruit Science, 2016, 33(7): 794-803.
[23] 李伟, 郜海燕, 陈杭君, 等. 基于主成分分析的不同品种杨梅果实综合品质评价[J]. 中国食品学报, 2017, 17(6): 161-171.
Li W, Gao H Y, Chen H J, et al.Evaluation of comprehensive quality of different varieties of bayberry based on principal components analysis[J]. Journal of Chinese Institute of Food Science and Technology, 2017, 17(6): 161-171.
[24] 李勋兰, 洪林, 王武, 等. 晚熟杂柑新品种果实品质综合评价[J]. 果树学报, 2018, 35(2): 195-203.
Li X L, Hong L, Wang W, et al.Comprehensive evaluation of fruit quality of new late-maturing mandarin cultivars[J]. Journal of Fruit Science, 2018, 35(2): 195-203.
[25] 吕正鑫, 贺艳群, 贾东峰, 等. 猕猴桃种质资源表型性状遗传多样性分析[J]. 园艺学报, 2022, 49(7): 1571-1581.
Lü Z X, He Y Q, Jia D F, et al.Genetic diversity analysis of phenotypic traits for kiwifruit germplasm resource[J]. Acta Horticulturae Sinica, 2022, 49(7): 1571-1581.
[26] 林子翔. 观赏辣椒种质资源园艺性状的多元统计分析与种质创新研究[D]. 杭州: 浙江农林大学, 2020.
Lin Z X.Multivariate statistical analysis and germplasm innovation of ornamental pepper germplasm resources [D]. Hangzhou: Zhejiang A&F University, 2020.
[27] 宁功伟, 杨盛美, 段志芬, 等. 云南野生茶树厚轴茶种质资源化学成分多样性分析[J/OL]. 分子植物育种, 2023: 1-20[2023-05-06]. https://kns.cnki.net/kcms/detail//46.1068.S.20230118.1701.005.html.
Ning G W, Yang S M, Duan Z F, et al. Diversity analysis of chemical components of wild tea germplasm resources(Camellia crassocolumna) in Yunnan [J/OL]. Molecular Plant Breeding, 2023: 1-20[2023-05-06]. https://kns.cnki.net/kcms/detail//46.1068.S.20230118.1701.005.html.
[28] 董方, 李小飞, 沈思言, 等. 江西茶树资源的遗传多样性分析及优异种质筛选[J]. 江西农业大学学报, 2022, 44(6): 1466-1477.
Dong F, Li X F, Shen S Y, et al.Genetic diversity analysis and screening of excellent germplasm of tea plant resources in Jiangxi[J]. Acta Agriculturae Universitatis Jiangxiensis, 2022, 44(6): 1466-1477.
[29] 丁帅涛, 程晓梅, 张亚, 等. 基于表型性状和生化成分的陕西茶树种质资源遗传多样性研究[J]. 西北农业学报, 2019, 28(4): 607-619.
Ding S T, Cheng X M, Zhang Y, et al.Genetic diversity in phenotypic traits and biochemical components of tea resources in Shanxi[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2019, 28(4): 607-619.
[30] Zhang Y R, Fu J M, Zhou Q Y, et al.Metabolite profiling and transcriptome analysis revealed the conserved transcriptional regulation mechanism of caffeine biosynthesis in tea and coffee germplasm plants[J]. Journal of Agricultural and Food Chemistry, 2022, 70(10): 3239-3251.
[31] Li G D, Li Y, Yao X Z, et al.Establishment of a virus-induced gene-silencing (VIGS) system in tea plant and its use in the functional analysis of CsTCS1[J]. International Journal of Molecular Sciences, 2023, 24(1): 392. doi: 10.3390/ijms24010392.
[32] Li P H, Ye Z L, Fu J M, et al.CsMYB184 regulates caffeine biosynthesis in tea plants[J]. Plant Biotechnology Journal, 2022, 20(6): 1012-1014.
[33] Ma W H, Kang X, Liu P, et al. The NAC-like transcription factor CsNAC7 positively regulates the caffeine biosynthesis-related gene yhNMT1 in Camellia sinensis [J]. Horticulture Research, 2022, 9: uhab046. doi: 10.1093/hr/uhab046.
[34] Jin J Q, Yao M Z, Ma C L, et al.Natural allelic variations of TCS1 play a crucial role in caffeine biosynthesis of tea plant and its related species[J]. Plant Physiology and Biochemistry, 2016, 100(12): 18-26.
[35] 蔡志翔, 严娟, 宿子文, 等. 不同类型桃种质资源主要酚类物质含量评价[J]. 园艺学报, 2022, 49(5): 1008-1022.
Cai Z X, Yan J, Su Z W, et al.Genetic diversity analysis of phenotypic traits for kiwifruit germplasm resources[J]. Acta Horticulturae Sinica, 2022, 49(5): 1008-1022.
[36] Zhu Q F, Liu L J, Lu X F, et al.The biosynthesis of EGCG, theanine and caffeine in response to temperature is mediated by hormone signal transduction factors in tea plant (Camellia sinensis L.)[J]. Frontiers in Plant Science, 2023, 14. doi: 10.3389/fpls.2023.1149182.
[37] 蒋会兵, 田易萍, 陈林波, 等. 云南茶树地方品种农艺性状与品质性状遗传多样性分析[J]. 植物遗传资源学报, 2013, 14(4): 634-640.
Jiang H B, Tian Y P, Chen L B, et al.Diversity of tea landraces based on agronomic and qualitytraits in Yunnan Province[J]. Journal of Plant Genetic Resources, 2013, 14(4): 634-640.
[38] 姚明哲. 利用ISSR和EST-SSR标记研究中国茶树资源的遗传多样性和遗传结构[D]. 杭州: 浙江大学, 2009.
Yao M Z.Studies on genetic diversity and structure of tea germplasm in China based on ISSR and EST-SSR markers [D]. Hangzhou: Zhejiang University, 2009.
[39] 陈勋, 龚自明. 基于EST-SSR标记的湖北茶树种质资源遗传多样性分析[J]. 分子植物育种, 2017, 15(5): 1831-1838.
Chen X, Gong Z M.Analysis of genetic diversity with EST-SSR markers for tea germplasm in Hubei Province[J]. Molecular Plant Breeding, 2017, 15(5): 1831-1838.
[40] 张晓丽, 郭辉, 王海岗, 等. 中国普通野生稻与栽培稻种SSR多样性的比较分析[J]. 作物学报, 2008, 105(4): 591-597.
Zhang X L, Guo H, Wang H G, et al.Comparative assessment of SSR allelic diversity in wild and cultivated rice in China[J]. Acta Agronomica Sinica, 2008, 105(4): 591-597.
[41] 刘振, 赵洋, 杨培迪, 等. 湖南省茶树种质资源现状及研究进展[J]. 茶叶通讯, 2011, 38(3): 7-10, 18.
Liu Z, Zhao Y, Yang P D, et al.The status and research progress of Hunan tea germplasm[J]. Tea Communication, 2011, 38(3): 7-10, 18.
[42] 马建强, 姚明哲, 陈亮. 茶树种质资源研究进展[J]. 茶叶科学, 2015, 35(1):11-16.
Ma J Q, Yao M Z, Chen L.Research progress on germplasms of tea plant (Camellia sinensis)[J]. Journal of Tea Science, 2015, 35(1):11-16.
[43] 张见明. 26份武夷名丛茶树种质资源秋茶生化成分多样性分析[J]. 武夷学院学报, 2016, 35(9): 1-7.
Zhang J M.Diversity analysis of biochemical components in autumn shoots of 26 Wuyi Mingcong tea plant germplasm resources[J]. Journal of Wuyi University, 2016, 35(9): 1-7.
[44] 乔婷婷. 茶树资源遗传多样性及其表型性状关联EST-SSR位点的初步鉴定[D]. 北京: 中国农业科学院, 2010.
Qiao T T.Genetic diversity of tea (Camellia sinensis (L.)O. Kuntze) and association analysis on phenotypic traits with EST-SSR markers [D]. Beijing: Chinese Academy of Agricultural Sciences, 2010.
[45] 齐冬晴. 不同茶树品种(品系)鲜叶香气及其表型差异的研究[D]. 长沙: 湖南农业大学, 2015.
Qi D Q.Studies on the tea leaves aroma components and phenotypic differences of different tea varieties [D]. Changsha: Hunan Agricultural University, 2015.