The Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of Thosea sinensis

JIANG Hongyan; CHEN Shichun; LIAO Shuran; CHEN Tingxu; YANG Puxiang; XIE Xiaoqun; WANG Xiaoqing

doi:10.13305/j.cnki.jts.2023.04.002

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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (4) : 460-472. DOI: 10.13305/j.cnki.jts.2023.04.002

Research Paper

The Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of Thosea sinensis

JIANG Hongyan¹, CHEN Shichun¹, LIAO Shuran¹, CHEN Tingxu¹, YANG Puxiang², XIE Xiaoqun², WANG Xiaoqing^1,*

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Abstract

Thosea sinensis is an important agricultural and forestry pest in China with characteristics of wide distribution, polyphagy, and high damage. The purpose of this study was to report the mitochondrial genome of T. sinensis collected from Jiangxi, investigate its diversity and difference, and explore the evolutionary characteristics of Limacodidae insects. After Sanger sequencing, the complete mitochondrial genome sequence of T. sinensis was obtained by splicing, correcting and annotating, and the phylogenetic tree of 26 moth species in 17 families of Lepidoptera was constructed based on the protein sequences. The complete mitochondrial genome sequence was 15 540 bp in size, encoding 37 genes, including 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNA genes, and 1 control region of 425 bp. The gene arrangement is the same as that of the Ditrysia moths. By comparing the similarity of the full sequence and protein-coding genes of the mitochondrial genomes with other moths, the results show that the similarity between T. sinensis and Iragoides fasciata was the highest, and that between T. sinensis and Parasa consocia was the lowest. Phylogenetic analysis shows that the closest relationship of T. sinensis was with Iragoides fasciata, followed by Narosa nigrisigna, and all the moths from Lepidoptera were clustered into one branch. This study provided a scientific basis for further research on the origin, genetic diversity, migration, and differentiation of T. sinensis, as well as its resistance to pesticides.

Key words

Thosea sinensis / Limacodidae / mitochondrial genome / phylogeny

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JIANG Hongyan, CHEN Shichun, LIAO Shuran, CHEN Tingxu, YANG Puxiang, XIE Xiaoqun, WANG Xiaoqing. The Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of Thosea sinensis[J]. Journal of Tea Science. 2023, 43(4): 460-472 https://doi.org/10.13305/j.cnki.jts.2023.04.002

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