有机种植对茶园土壤微生物群落组成与多样性的影响

doi:10.13305/j.cnki.jts.2024.02.003

Abstract

Abstract: To reveal the influence of organic planting on the community composition and diversity of soil microbial community, 4 types of soils (ancient tea gardens, modern tea gardens, rubber fields, wastelands) were used as the research objects in 3 representative ancient tea mountains (Manzhuan, Yiwu and Youle) in Mengla County, Yunnan Province. The community composition of bacteria and fungi were identified using Illumina MiSeq PE300 high-throughput sequencing technology. The effects of organic planting and planting years on soil physical and chemical properties, microbial community composition characteristics and diversity were analyzed. The results show that organic planting could promote soil organic matter accumulation and increase the contents of nitrogen, phosphorus and potassium nutrients. The common number of bacteria OTUs in the soils of three tea gardens and non-tea gardens was 381. Among them, Yiwu Wasteland had highest number of unique OTUs (293), while the Rubber Land of Manzhuan had the lowest number of unique OTUs (28). The total number of fungi OTUs was only 24, with the highest number of fungi OTUs unique to Manzhuan ancient tea garden (337) and the lowest number of OTUs unique to Yiwu Modern Tea Garden (55). In addition, The Shannon diversity index of bacteria reached 5.88-6.62, which was significantly higher than that of fungi (2.71-4.30). The dominant bacteria and fungi in tea garden soils were basically similar to those in non-tea garden soil. However, there were significant differences in relative abundance among identified Acidimicrobiales, Bradyrhizobium, Varibacter, Xanthobacteraceae, Nitrospira, Bryobacter, Acidibacter and Planomyceteaceae among different tea mountains and land use modes. Compared with wasteland and rubber land, the relative abundance of Chaetomiaceae, Penicillium, Fusarium, Trichoderma, Mortierella, Agaricales and Eurotiomycetes in tea garden soil fungal communities were significantly higher than those in other soils. The abundance index of bacterial community Chao1 was significantly and positively correlated with soil TN and TP, and the bacterial community composition was more stable than the fungal community composition of the three mountains with ancient tea plants. Except for some tea garden soils, the abundance of soil bacteria increased with the increase of organic planting and planting years, while the abundance of fungi decreased and then increased with the beginning of organic planting. The diversity level of bacteria and fungi in modern and ancient tea gardens decreased with the increase of organic planting and planting years.

Key words: tea garden, soil microorganisms, community composition, diversity, high-throughput sequencing

CLC Number:

S517.1

YAN Yuxiao, ZHOU Dapeng, YANG Yanfen, Xie Jin, LÜ Caiyou, YANG Guangrong, WEN Qinshu. Influence of Organic Planting on Soil Microbial Community Composition and Diversity in Tea Gardens[J]. Journal of Tea Science, 2024, 44(2): 246-260.

References

[1] 江志阳, 高立红, 尹微. 土壤微生物: 可持续农业和环境发展的新维度[J]. 微生物学杂志, 2020, 40(3): 1-7.
Jiang Z Y, Gao L H, Yin W.Soil microorganisms: a new dimension of sustainable agriculture and environmental development[J]. Journal of Microbiology, 2020, 40(3): 1-7.
[2] 林先贵, 胡君利. 土壤微生物多样性的科学内涵及其生态服务功能[J]. 土壤学报, 2008(5): 892-900.
Lin X G, Hu J L.The scientific connotation of soil microbial diversity and its ecological service function[J]. Acta Pedologica Sinica, 2008(5): 892-900.
[3] 顾松松, 胡秋龙, 刘仲华, 等. 不同类型茶园土壤细菌多样性及群落结构研究[J]. 茶叶通讯, 2019, 46(2): 162-169.
Gu S S, Hu Q L, Liu Z H, et al.The study on diversity and community structure of organic, non-polluted and common tea plantations[J]. Journal of Tea Communication, 2019, 46(2): 162-169.
[4] 韩文炎, 王皖蒙, 郭赟, 等. 茶园土壤细菌丰度及其影响因子研究[J]. 茶叶科学, 2013, 33(2): 147-154.
Han W Y, Wang W M, Guo Y, et al.Bacterial abundance of tea garden soils and its influencing factors[J]. Journal of Tea Science, 2013, 33(2): 147-154.
[5] 周才碧, 陈文品. 茶园土壤微生物的研究进展[J]. 中国茶叶, 2014, 36(3): 14-15.
Zhou C B, Chen W P.Research progress of soil microorganisms in tea gardens[J]. China Tea, 2014, 36(3): 14-15.
[6] 王海斌, 陈晓婷, 丁力, 等. 土壤酸度对茶树根际土壤微生物群落多样性影响[J]. 热带作物学报, 2018, 39(3): 448-454.
Wang H B, Chen X T, Ding L, et al.Effect of soil acidity on microbial diversity in rhizospheric soils of tea plants[J]. Chinese Journal of Tropical Crops, 2018, 39(3): 448-454.
[7] 林生, 庄家强, 陈婷, 等. 不同年限茶树根际土壤微生物群落PLFA生物标记多样性分析[J]. 生态学杂志, 2013, 32(1): 64-71.
Lin S, Zhuang J Q, Chen T, et al.Microbial diversity in rhizosphere soils of different planting year tea trees: an analysis with phospholipid fatty acid biomarkers[J]. Chinese Journal of Ecology, 2013, 32(1): 64-71.
[8] Li W, Zheng Z C, Li T X, et al.Effect of tea plantation age on the distribution of soil organic carbon fractions within water-stable aggregates in the hilly region of Western Sichuan, China[J]. CATENA, 2013, 133: 198-205.
[9] 姚泽秀, 李永春, 李永夫, 等. 植茶年限对土壤微生物群落结构及多样性的影响[J]. 应用生态学报, 2020, 31(8): 2749-2758.
Yao Z X, Li Y C, Li Y F, et al.Effects of different tea plantation ages on soil microbial community structure and diversity[J]. Chinese Journal of Applied Ecology, 2020, 31(8): 2749-2758.
[10] Alekseeva T, Alekseev A, Xu R K et al. Effect of soil acidification induced by a tea plantation on chemical and mineralogical properties of Alfisols in eastern China[J]. Environmental Geochemistry & Health, 2020, 33(2): 137-148.
[11] 陈丽华, 刘兰英, 吕新, 等. 不同种植年限茶树根际土壤微生物数量和肥力之间关系[J]. 福建农业学报, 2019, 34(12): 1433-1439.
Chen L H, Liu L Y, Lü X, et al.Microbial population and fertility of rhizosphere soils at areas of varied tea-planting years[J]. Fujian Journal of Agriculture, 2019, 34(12): 1433-1439.
[12] 汪华. 茶园土壤微生物群落结构对植茶年限、施肥和高温条件的响应研究[D]. 杭州: 浙江大学, 2015.
Wang H.The response of soil microbial structure to cultivating age, fertilization and high temperature in tea orchard [D]. Hangzhou: Zhejiang University, 2015.
[13] 杨广容, 马燕, 蒋宾, 等. 基于16S rDNA测序对茶园土壤细菌群落多样性的研究[J]. 生态学报, 2019, 39(22): 8452-8461.
Yang G R, Ma Y, Jiang, et al. Analysis of the bacterial community and diversity in tea plantation soil via 16S rDNA sequencing[J]. Acta Ecologica Sinica, 2019, 39(22): 8452-8461.
[14] Zhao J, Wu X B, Nie C P, et al.Analysis of unculturable bacterial communities in tea orchard soils based on nested PCR-DGGE[J]. World Journal of Microbiology and Biotechnology, 2012, 28(5): 1967-1979.
[15] 胡磊, 杨广, 尤民生. 茶园土壤微生物总DNA不同提取方法的比较[J]. 基因组学与应用生物学, 2010, 29(2): 361-368.
Hu L, Yang G, You M S.Comparison of different DNA extraction methods for soil microbes in tea plantations[J]. Genomics and Applied Biology, 2010, 29(2): 361-368.
[16] 鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2005.
Bao S D. Soil Agrochemical Analysis.[M]. 3rd ed. Beijing: China Agriculture Press, 2005.
[17] 李立平, 邢维芹, 成永霞, 等. 华北平原中部典型农区不同土地利用方式下的土壤性质比较[J]. 土壤通报, 2020, 51(6): 1275-1281.
Li L P, Xing W Q, Cheng Y X, et al.Comparison of soil properties under different land use types in the typical farming areas of middle north China plain[J]. Chinese Journal of Soil Science, 2020, 51(6): 1275-1281.
[18] 梁博, 林田苗, 任德智, 等. 土地利用方式对雅江中游土壤理化性质及颗粒分形特征的影响[J]. 土壤, 2018, 50(3): 613-621.
Liang B, Lin T M, Ren D Z, et al.Efects of land use types on soil physicochemical properties and fractal characteristics of soil particles in midde reaches of Yajiang River[J]. Soils, 2018, 50(3): 613-621.
[19] 梁名志, 李友勇, 马伟, 等. 云南古茶园土壤化学成分研究[J]. 西南农业学报, 2010, 23(1): 119-122.
Liang M Z, Li Y Y, Ma W, et al.Study on soil chemical constituents of ancient tea gardens in Yunnan Province[J]. Southwest China Journal of Agricultural Sciences, 2010, 23(1): 119-122.
[20] 彭萍, 杨水平, 李品武, 等. 植茶对土壤环境效应分析研究[J]. 茶叶科学, 2007, 27(3): 265-270.
Peng P, Yang S P, Li P W, et al.Effects of tea planting on the yellow soil properties in tea garden[J]. Journal of Tea Science, 2007, 27(3): 265-270.
[21] 李倩. 地质背景及土壤养分与崂山绿茶品质研究[D]. 青岛: 青岛农业大学, 2010.
Li Q.Research on geological background and soil nutrients of Laoshan green tea and its ouality [D]. Qingdao: Qingdao Agricultural University, 2010.
[22] Acosta-martinez V, Burow G, Zobeck T M, et al. Soil microbial communities and function in alternative systems to continuous cotton[J]. Soil Science Society of America Journal, 2010, 74(4): 1181-1192.
[23] Tedersoo L, Nilsson R H, Abarenkov K, et al.454 pyrosequencing and sanger sequencing of tropical mycorrhizal fungi provide similar results but reveal substantial methodological biases[J]. New Phytologist, 2010, 188(1): 291-301.
[24] Alvey S, Yang C H, Burkert A, et al.Cereal/legume rotation effects on rhizosphere bacterial community structure in West African soils[J]. Biology & Fertility of Soil, 2003, 37(2): 73-82.
[25] 张丽红, 符建平, 高丽红, 等. 不同蔬菜轮作对日光温室土壤微生物的影响[J]. 中国农学通报, 2010, 26(1): 140-144.
Zhang L H, Fu J P, Gao L H, et al.The effects of different vegetable rotations on soil microorganisms in solar greenhouses[J]. Chinese Agricultural Science Bulletin, 2010, 26(1): 140-144.
[26] Wang S Q, Li T X, Zheng Z C, et al.Soil aggregate-associated bacterial metabolic activity and community structure in different aged tea plantations[J]. Science of The Total Environment, 2019, 654: 1023-1032.
[27] 卢开阳, 张云峰, 贾鳗, 等. 基于16S rRNA序列分析南糯山不同生境茶树根际土壤细菌群落分布多样性[J]. 昆明理工大学学报(自然科学版), 2016, 41(2): 89-95.
Lu K Y, Zhang Y F, Jia M, et al.Bacteria distribution diversity in tea rhizospheric soil from diferent habitats at Nannuo mountain based on 16S rRNA sequence analysis[J]. Journal of Kunming University of Science and Technology (Natural Science), 2016, 41(2): 89-95.
[28] Kanokratana P, Uengwetwanit T, Rattanachomsri U, et al.Insights into the phylogeny and metabolic potential of a primary tropical peat swamp forest microbial community by metagenomic analysis[J]. Microbial Ecology, 2011, 61(3): 518-528.
[29] 王秀青, 李永梅, 谢瑾, 等. 古茶园和现代茶园土壤养分与微生物数量的研究[J]. 西南大学学报(自然科学版), 2015, 37(10): 43-50.
Wang X Q, Li Y M, Xie J, et al.Study on soil nutrients and quantity of microbial community of ancient tea arboretums and modern tea gardens[J]. Journal of Southwest University (Natural Science Edition), 2015, 37(10): 43-50.
[30] 季凌飞, 倪康, 马立锋, 等. 不同施肥方式对酸性茶园土壤真菌群落的影响[J]. 生态学报, 2018, 38(22): 8158-8166.
Ji L F, Ni K, Ma L F, et al.Effects of different fertilization methods on soil fungal communities in acid tea gardens[J]. Acta Ecologica Sinica, 2018, 38(22): 8158-8166.
[31] 胡雲飞, 张玥, 张彩丽, 等. 茶树根系内生真菌与根际土壤真菌的季节多样性分析[J]. 南京农业大学学报, 2013, 36(3): 41-46.
Hu Y F, Zhang Y, Zhang C L, et al.Seasonal diversity analysis of tea root endophytic fungi and rhizosphere soil fungi[J]. Journal of Nanjing Agricultural University, 2013, 36(3): 41-46.
[32] 袁赛艳, 陈暄, 房婉萍, 等. 茶树修剪物对茶园土壤真菌群落多样性影响研究[J]. 茶叶科学, 2015, 35(3): 255-262.
Yuan S Y, Chen X, Fang W P, et al.Study on the influence of tea pruning materials on the diversity of soil fungal communities in tea gardens[J]. Journal of Tea Science, 2015, 35(3): 255-262.
[33] 郭彦青. 黄土高原退耕还草区土壤微生物群落研究[D]. 杨凌: 西北农林科技大学, 2017.
Guo Y Q.Dynamics of soi microbial communities inrevegetation areas on the Loess Plateau of China [D]. Yangling: Northwest A&F University, 2017.
[34] Puget P, Angers D A, Chenu C.Nature of carbohydrates associated with water-stable aggregates of two cultivated soils[J]. Soil Biology and Biochemistry, 1998, 31(1): 55-63.
[35] 张坤, 包维楷, 杨兵, 等. 林下植被对土壤微生物群落组成与结构的影响[J]. 应用与环境生物学报, 2017, 23(6): 1178-1184.
Zhang K, Bao W K, Yang B, et al.The influence of understory vegetation on the composition and structure of soil microbial communities[J]. Journal of Applied and Environmental Biology, 2017, 23(6): 1178-1184.
[36] 刘丽, 徐明恺, 汪思龙, 等. 杉木人工林土壤质量演变过程中土壤微生物群落结构变化[J]. 生态学报, 2013, 33(15): 4692-4706.
Liu L, Xu M K, Wang S L, et al.Fffect of different Cunninghamia lanceolata plantation soil qualities on soil microbial community structure[J]. Acta Ecologica Sinica, 2013, 33(15): 4692-4706.
[37] 刘晶, 赵燕, 张巧明, 等. 不同利用方式对豫西黄土丘陵区土壤微生物生物量及群落结构特征的影响[J]. 草业学报, 2016, 25(8): 36-47.
Liu J, Zhao Y, Zhang Q M, et al.Effects of land use on soil microbial biomass and community structure in the loess hill region of west Henan[J]. Acta Prataculturae Sinica, 2016, 25(8): 36-47.
[38] 李鑫, 周冀衡, 贺丹锋, 等. 干旱胁迫下枸溶性钾肥配施对烤烟土壤理化性质、微生物数量及根系生长的影响[J]. 核农学报, 2016, 30(12): 2434-2440.
Li X, Zhou J H, He D F, et al.Effects of citrate soluble potassium fertilizer on soil physicochemical properties of flue-cured tobacco, microbial quantity and root growth under drought stress[J]. Journal of Nuclear Agriculture, 2016, 30(12): 2434-2440.

Influence of Organic Planting on Soil Microbial Community Composition and Diversity in Tea Gardens

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