Influence of Organic Planting on Soil Microbial Community Composition and Diversity in Tea Gardens

YAN Yuxiao; ZHOU Dapeng; YANG Yanfen; Xie Jin; L&#x000dc; Caiyou; YANG Guangrong; WEN Qinshu

doi:10.13305/j.cnki.jts.2024.02.003

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Journal of Tea Science ›› 2024, Vol. 44 ›› Issue (2) : 246-260. DOI: 10.13305/j.cnki.jts.2024.02.003

Research Paper

Influence of Organic Planting on Soil Microbial Community Composition and Diversity in Tea Gardens

YAN Yuxiao¹, ZHOU Dapeng¹, YANG Yanfen¹, Xie Jin¹, LÜ Caiyou¹, YANG Guangrong¹, WEN Qinshu^2,*

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Abstract

To reveal the influence of organic planting on the community composition and diversity of soil microbial community, 4 types of soils (ancient tea gardens, modern tea gardens, rubber fields, wastelands) were used as the research objects in 3 representative ancient tea mountains (Manzhuan, Yiwu and Youle) in Mengla County, Yunnan Province. The community composition of bacteria and fungi were identified using Illumina MiSeq PE300 high-throughput sequencing technology. The effects of organic planting and planting years on soil physical and chemical properties, microbial community composition characteristics and diversity were analyzed. The results show that organic planting could promote soil organic matter accumulation and increase the contents of nitrogen, phosphorus and potassium nutrients. The common number of bacteria OTUs in the soils of three tea gardens and non-tea gardens was 381. Among them, Yiwu Wasteland had highest number of unique OTUs (293), while the Rubber Land of Manzhuan had the lowest number of unique OTUs (28). The total number of fungi OTUs was only 24, with the highest number of fungi OTUs unique to Manzhuan ancient tea garden (337) and the lowest number of OTUs unique to Yiwu Modern Tea Garden (55). In addition, The Shannon diversity index of bacteria reached 5.88-6.62, which was significantly higher than that of fungi (2.71-4.30). The dominant bacteria and fungi in tea garden soils were basically similar to those in non-tea garden soil. However, there were significant differences in relative abundance among identified Acidimicrobiales, Bradyrhizobium, Varibacter, Xanthobacteraceae, Nitrospira, Bryobacter, Acidibacter and Planomyceteaceae among different tea mountains and land use modes. Compared with wasteland and rubber land, the relative abundance of Chaetomiaceae, Penicillium, Fusarium, Trichoderma, Mortierella, Agaricales and Eurotiomycetes in tea garden soil fungal communities were significantly higher than those in other soils. The abundance index of bacterial community Chao1 was significantly and positively correlated with soil TN and TP, and the bacterial community composition was more stable than the fungal community composition of the three mountains with ancient tea plants. Except for some tea garden soils, the abundance of soil bacteria increased with the increase of organic planting and planting years, while the abundance of fungi decreased and then increased with the beginning of organic planting. The diversity level of bacteria and fungi in modern and ancient tea gardens decreased with the increase of organic planting and planting years.

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community composition / diversity / high-throughput sequencing / soil microorganisms / tea garden

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YAN Yuxiao, ZHOU Dapeng, YANG Yanfen, Xie Jin, LÜ Caiyou, YANG Guangrong, WEN Qinshu. Influence of Organic Planting on Soil Microbial Community Composition and Diversity in Tea Gardens[J]. Journal of Tea Science. 2024, 44(2): 246-260 https://doi.org/10.13305/j.cnki.jts.2024.02.003

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