Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City

WANG Liubin; HUANG Liyun; TENG Cuiqin; WU Liyun; CHENG Hao; YU Cuiping; WANG Liyuan

doi:10.13305/j.cnki.jts.2022.05.004

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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (5) : 601-609. DOI: 10.13305/j.cnki.jts.2022.05.004

Research Paper

Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City

WANG Liubin¹, HUANG Liyun², TENG Cuiqin², WU Liyun¹, CHENG Hao¹, YU Cuiping^2,*, WANG Liyuan^1,*

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Abstract

Based on the SSR markers, the genetic diversity and genetic relationship of the germplasm resources of tea plants from Liubao town and Nandu town were fully analyzed in this study. The core molecular markers for the efficient identification of these germplasm resources were successfully screened. The main results show that: (1) 98 alleles were amplified from 17 pairs of SSR primers, and each pair of SSR primers amplified 3-8 alleles, with an average of 5.764 7 alleles per locus. (2) Totally 8 core markers were selected from 17 SSR markers to distinguish each germplasm resource. (3) The average number of alleles (4.647 1), genotypes per marker (7.000 0), genetic diversity (0.675 4), and the polymorphic information content (0.628 3) of native tea trees in Liubao town were higher than wild tea trees in Nandu, and close to the cultivated population. (4) Cluster analysis shows that the majority germplasm resources from Liubao town were clustered together except for several tea plants grouped with large-leaf tea cultivars from Yunnan province and a few resources were grouped into the same cluster with tea cultivars from Zhejiang and Guizhou provinces. The wild tea germplasm resources from Nandu town were grouped into the same cluster with two germplasm materials from Liubao town. In conclusion, it was showed that there are rich tea germplasm resources with high genetic diversity in Wuzhou city. This study might lay a solid foundation for the further studies to develop and utilize these tea resources.

Key words

Camellia sinensis / genetic diversity / SSR marker / Wuzhou city

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WANG Liubin, HUANG Liyun, TENG Cuiqin, WU Liyun, CHENG Hao, YU Cuiping, WANG Liyuan. Genetic and Phylogenetic Analysis for Germplasm Resources of Camellia sinensis from Wuzhou City[J]. Journal of Tea Science. 2022, 42(5): 601-609 https://doi.org/10.13305/j.cnki.jts.2022.05.004

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