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Journal of Tea Science ›› 2024, Vol. 44 ›› Issue (2): 207-218.doi: 10.13305/j.cnki.jts.2024.02.001

• Research Paper • Previous Articles     Next Articles

The Development of CAPS Molecular Markers for CsAL1, A Gene Associated with Early and Late Spring Tip Emergence in Tea Plants

HUANG Mengdi1, CHEN Lan1, SU Qin1, HU Jinyu1, LIU Guizhi1, TAN Yueping3, LIU Shuoqian1,2,*, TIAN Na1,2,*   

  1. 1. Key Laboratory of Tea Science of Ministry of Education, Changsha 410128, China;
    2. Department of Tea Science, College of Horticulture, Hunan Agricultural University, Changsha 410128, China;
    3. Hunan Tea Group Co., Ltd., Changsha 410126, China
  • Received:2024-01-26 Revised:2024-03-25 Online:2024-04-15 Published:2024-04-30

Abstract: Camellia sinensis is an economically important foliar plant. The time of spring sprouting is a crucial biological trait that affects the economic value of tea. Therefore, selecting and breeding early sprouting tea cultivars are of great practical significance for improving the quality and economic benefits of tea. The study used ‘Tieguanyin’ as the reference genome and screened a gene, CsAL1 (Auxilin-like 1, TGY040711), which was highly significantly correlated with the time of spring sprouting based on genome-wide association analysis. SNP calling was used to obtain SNPs of CsAL1 in each sample. Correlation analysis of the SNPs and spring sprouting phenotypes was performed to obtain the key SNP loci that associating with spring sprouting traits. Suitable enzyme cleavage sites were analyzed for each SNP to develop CAPS molecular markers related to early-sprouting traits in tea plants. PCR was used to amplify the CAPS molecular markers, which were then digested in the genomic DNA of 12 tea materials. The markers were further verified in 72 tea materials to provide a reference for the association between single-base mutations in CsAL1 and early sprouting traits using CAPS molecular markers. This study also provided theoretical support for the breeding of early sprouting tea cultivars.

Key words: Camellia sinensis, SNP, CAPS molecular marker, early sprouting, molecular marker breeding

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