Genetic Diversity and Population Structure of Wild and Cultivated Camellia taliensis Populations

MAO Juan; JIANG Hongjian; YANG Rubing; LI Chongxing; MA Chengying; CHEN Liang; MA Jianqiang

doi:10.13305/j.cnki.jts.2021.04.002

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (4) : 454-462. DOI: 10.13305/j.cnki.jts.2021.04.002

Research Paper

Genetic Diversity and Population Structure of Wild and Cultivated Camellia taliensis Populations

MAO Juan^1,2, JIANG Hongjian³, YANG Rubing⁴, LI Chongxing⁵, MA Chengying⁶, CHEN Liang¹, MA Jianqiang^1,*

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Abstract

Camellia taliensis is a wild relative of tea plants (C. sinensis). Understanding of the genetic diversity and population structure of Camellia taliensis is important and helpful for protecting and utilizing these germplasm resources. In this study, a set of 30 core SSR markers derived from tea plants were used for genetic analysis of three representative wild and cultivated C. taliensis populations. The results show that all SSR markers successfully yielded specific amplification, with a range of polymorphic alleles from 2 to 14. The PIC value was between 0.041 and 0.877, with an average of 0.491. The three C. taliensis populations showed moderate levels of genetic diversity, and it was relatively lower for the Daxueshan (DXS) wild population. The inbreeding coefficient (Fis) of Xiangzhuqing (XZQ) and Baiyingshan (BYS) cultivated populations were 0.728 and 0.913 respectively, which meant high levels of inbreeding. Pairwise comparisons of the genetic differentiation index of three populations were low (Fst<0.15), while the rates of gene flow were high (Nm>1). The results of AMOVA exhibite that 94.1% of the genetic variation was within population. The genetic distances of wild and cultivated C. taliensis individuals were relatively higher. The genetic background of wild population was similar, while it was complex for cultivated populations. Seven individuals of BYS population were possibly originated from hybridization and introgression between C. taliensis and C. sinensis var. assamica.

Key words

Camellia taliensis / genetic diversity / population structure / SSR

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MAO Juan, JIANG Hongjian, YANG Rubing, LI Chongxing, MA Chengying, CHEN Liang, MA Jianqiang. Genetic Diversity and Population Structure of Wild and Cultivated Camellia taliensis Populations[J]. Journal of Tea Science. 2021, 41(4): 454-462 https://doi.org/10.13305/j.cnki.jts.2021.04.002

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