茶树BZR1基因家族的鉴定及CsBZR1-5响应干旱胁迫的分子机理研究

doi:10.13305/j.cnki.jts.2025.01.004

Abstract

Abstract: The BZR1 transcription factor is a key transcription factor in the brassinosteroid (BR) signaling pathway, playing an important regulatory role in plant growth, development, and stress response. This study identified and cloned six members of the BZR1 family in tea plants based on genomic data. Their gene structures, subcellular localization of encoded proteins, and transcriptional activation activities were analyzed, and their expression patterns under different tissues and drought stress were explored. The results show that the number of introns in the 6 BZR1 members of tea plants was 2 or 3, and their encoded proteins all contained typical bHLH characteristic structural domains. Subcellular localization analysis shows that except for CsBZR1-1, which was localized in the cytoplasm and nucleus, all other CsBZR1s were localized in the nucleus. Transcriptional activation activity analysis shows that CsBZR1s exhibited transcriptional activation activity in yeast. The analysis of expression patterns in different tissues shows that CsBZR1s had specificity in expression in different tissues of tea plants, among which the expression patterns of CsBZR1-1 and CsBZR1-6 were relatively similar. The expression pattern analysis under drought stress shows that all six CsBZR1 genes were responsive to drought stress. The expression of CsBZR1-5 was continuously induced by drought stress simulated by PEG. In addition, the expression pattern of the key enzyme gene CsNCED1 in ABA synthesis pathway was highly similar to that of CsBZR1-5 under drought stress. The analysis of Electrophoretic Mobility Shift Assay (EMSA) found that CsBZR1-5 can bind to the E-box element on the CsNCED1 promoter, indicating that CsBZR1-5 may be involved in regulating the response of CsNCED1 to drought stress. This study systematically analyzed the basic characteristics and functions of six CsBZR1 members, laying the foundation for further elucidating the regulatory roles of CsBZR1 members in tea plant growth and development and drought stress response.

Key words: tea plant, CsBZR1s gene, subcellular localization, transcriptional self-activation, EMSA

CLC Number:

S571.1
Q946

DONG Yuan, ZHANG Yongheng, XIAO Yezi, YU Youben. Cloning of BZR1 Gene Family in Tea Plants and Molecular Mechanism Study of CsBZR1-5 Response to Drought Stress[J]. Journal of Tea Science, 2025, 45(1): 15-28.

References

[1] Yin Y, Vafeados D, Tao Y, et al.A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis[J]. Cell, 2005, 120: 249-259.
[2] Ye H, Liu S, Tang B, et al.RD26 mediates crosstalk between drought and brassinosteroid signalling pathways[J]. Nature Communications, 2017, 8(1): 14573. doi: 10.1038/ncomms14573.
[3] 郭新磊, 路普, 王园园, 等. 棉花BZR基因家族的全基因组鉴定及表达分析[J]. 棉花学报, 2017, 29(5): 415-427.
Guo X L, Lu P, Wang Y Y, et al.Genome-wide identification and expression analysis of gene family encoding brassinazole resistant transcription factors in cotton[J]. Cotton Science, 2017, 29(5): 415-427.
[4] Saha G, Park J I, Jung H J, et al.Molecular characterization of BZR transcription factor family and abiotic stress induced expression profiling in Brassica rapa[J]. Plant Physiology Biochemistry, 2015, 92: 92-104.
[5] Yin Y L, Qin K Z, Song X W, et al.BZR1 transcription factor regulates heat stress tolerance through FERONIA receptor-like kinase-mediated reactive oxygen species signaling in tomato[J]. Plant Cell Physiology, 2018, 59(11): 2239-2254.
[6] Bai M Y, Zhang L Y, Gampala S S, et al.Functions of OsBZR1 and 14-3-3 proteins in brassinosteroid signaling in rice[J]. PNAs, 2007, 104(34): 13839-13844.
[7] Park C H, Kim T W, Son S H, et al.Brassinosteroids control AtEXPA5 gene expression in Arabidopsis thaliana[J]. Phytochemistry, 2010, 71(4): 380-387.
[8] Lachowiec J, Mason G A, Schultz K, et al.Redundancy, feedback, and robustness in the Arabidopsis thaliana BZR/BEH gene family[J]. Front Genet, 2018, 9: 523. doi: 10.3389/fgene.2018.00523.
[9] 刘天宇. 油菜素甾醇对番茄保卫细胞运动的影响及其调控机制[D]. 杭州: 浙江大学, 2016.
Liu T Y.Effect and regulatory mechanism of brassinosteroid on stomatal movement in tomato [D]. Hangzhou: Zhejiang University, 2016.
[10] Domagalska M A, Schomburg F M, Amasino R M, et al.Attenuation of brassinosteroid signaling enhances FLC expression and delays flowering[J]. Development, 2007, 134(15): 2841-2850.
[11] Jiang W B, Huang H Y, Hu Y W, et al.Brassinosteroid regulates seed size and shape in Arabidopsis[J]. Plant Physiology, 2013, 162(4): 1965-1977.
[12] Jiang J, Zhang C, Wang X.A recently evolved isoform of the transcription factor BES1 promotes brassinosteroid signaling and development in Arabidopsis thaliana[J]. Plant Cell. 2015, 27(2): 361-374.
[13] 陈旭, 沈春洋, 莫福磊, 等. 番茄BZR基因家族鉴定及非生物胁迫下表达模式分析[J]. 东北农业大学学报, 2021, 52(11): 9-17.
Chen X, Shen C Y, Mo F L, et al.Identification of BZR gene family in tomato and expression patterns analysis under abiotic stress[J]. Journal of Northeast Agricultural University, 2021, 52(11): 9-17.
[14] Yu H Q, Feng W Q, Sun F A, et al.Cloning and characterization of BES1/BZR1 transcription factor genes in maize[J]. Journal of Plant Growth Regulation, 2018, 86: 235-249.
[15] Luo S L, Zhang G B, Zhang Z Y, et al.Genome-wide identification and expression analysis of BZR gene family and associated responses to abiotic stresses in cucumber (Cucumis sativus L.)[J]. BMC Plant Biology, 2023, 23(1): 214. doi: 10.1186/s12870-023-04216-9.
[16] 李春, 刘小俊, 蔡鹏, 等. 中国南瓜BZR基因家族的全基因组鉴定及生物信息学分析[J]. 分子植物育种, 2022, 20(19): 6324-6330.
Li C, Liu X J, Cai P, et al.Genome-wide identification and bioinformatics analysis of BZR gene family in pumpkin (Cucurbita moschata Duch.)[J]. Molecular Plant Breeding, 2022, 20(19): 6324-6330.
[17] Li S, Yan J, Chen L G, et al.Brassinosteroid regulates stomatal development in etiolated Arabidopsis cotyledons via transcription factors BZR1 and BES1[J]. Plant Physiology, 2024, 195(2): 1382-1400.
[18] Diao R, Zhao M, Liu Y, et al.The advantages of crosstalk during the evolution of the BZR1-ARF6-PIF4 (BAP) module[J]. Journal of Integrative Plant Biology, 2023, 65(12): 2631-2644.
[19] He Y, Zhao Y, Hu J, et al.The OsBZR1-OsSPX1/2 module fine-tunes the growth-immunity trade-off in adaptation to phosphate availability in rice[J]. Molecular Plant, 2024, 17(2): 258-276.
[20] Wang Y, Cao J, Wang K, et al.BZR1 Mediates brassinosteroid-induced autophagy and nitrogen starvation in tomato[J]. Plant Physiology, 2018, 179: 671-685.
[21] 江倩倩, 王雨婷, 惠竹梅. 葡萄BZR基因家族的鉴定及表达分析[J]. 植物生理学报, 2021, 57(6): 1218-1228.
Jiang Q Q, Wang Y T, Xi Z M.Identification and expression analysis of BZR gene family in grapevine[J]. Plant Physiology Journal, 2021, 57(6): 1218-1228.
[22] 黎泽斌, 邱永争, 刘延杰, 等. 紫花苜蓿BZR基因家族鉴定及其对非生物胁迫的响应分析[J]. 草业学报, 2024, 33(11): 106-122.
Li Z B, Qiu Y Z, Liu Y J, et al.Identification of the BZR gene family in alfalfa and analysis of its transcriptional responses to abiotic stress[J]. Acta Prataculturae Sinica, 2024, 33(11): 106-122.
[23] Sun F, Yu H, Qu J, et al.Maize ZmBES1/BZR1-5 decreases ABA sensitivity and confers tolerance to osmotic stress in transgenic Arabidopsis[J]. International Journal of Molecular Sciences, 2020, 21(3): 996. doi: 10.3390/ijms21030996.
[24] Hwang S G, Lee C Y, Tseng C S.Heterologous expression of rice 9-cis-epoxycarotenoid dioxygenase 4 (OsNCED4) in Arabidopsis confers sugar oversensitivity and drought tolerance[J]. Botanical Studies, 2018, 59(1): 2. doi:10.1186/s40529-018-0219-9.
[25] 刘建汀, 叶新如, 张前荣, 等. 西葫芦NCED基因家族鉴定及其响应干旱胁迫分析[J]. 西北植物学报, 2023, 43(4): 569-581.
Liu J T, Ye X R, Zhang Q R, et al.Genome-wide identification and response to drought stress of NCED genes family in Zucchini (Cucurbita pepo L.)[J]. Acta Botanica Boreali-Occidentalia Sinica, 2023, 43(4): 569-581.
[26] Li J W, Zhou P, Yang N, et al.CsBZR1 family transcription factors in wild and cultural tea plants and their response to hormone and abiotic stress[J]. Journal of Plant Growth Regulation, 2024, 43: 840-853.
[27] Zhang Q J, Li W, Li K, et al.The Chromosome-level reference genome of tea tree unveils recent bursts of non-autonomous LTR retrotransposons in driving genome size evolution[J]. Molecular Plant, 2024, 13(7): 935-938.
[28] Yu X, Li L, Zola J, et al.A brassinosteroid transcriptional network revealed by genome-wide identification of BESI target genes in Arabidopsis thaliana[J]. Plant Journal, 2011, 65(4): 634-646.
[29] He J X, Gendron J M, Sun Y, et al.BZR1 is a transcriptional repressor with dual roles in brassinosteroid homeostasis and growth responses[J]. Science, 2005, 307(5715): 1634-1638.
[30] Zhang Y H, Xiao Y Z, Zhang Y A, et al.Accumulation of Galactinol and ABA is involved in exogenous EBR-induced drough tolerance in tea plants[J]. Journal of Agricultural and Food Chemistry, 2022, 70(41): 13391-13403.
[31] 臧文蕊, 马明, 砗根, 等. 甜瓜BZR转录因子家族基因的全基因组鉴定及表达模式分析[J]. 生物技术通报, 2024, 40(7): 163-171.
Zang W R, Ma M, Chen G, et al.Genome-wide identification and expression pattern analysis of BZR transcription factor gene family of melon[J]. Biotechnology Bulletin, 2024, 40(7): 163-171.
[32] Luo S, Zhang G, Zhang Z, et al.Genome-wide identification and expression analysis of BZR gene family and associated responses to abiotic stresses in cucumber (Cucumis sativus L.)[J]. BMC Plant Biology, 2023, 23: 214. doi: 10.1186/s12870-023-04216-9.
[33] 张晴, 严新悦, 左春柳, 等. 大豆BZR1基因家族进化与油菜素内酯响应分析[J]. 河北师范大学学报(自然科学版), 2023, 47(6): 620-627.
Zhang Q, Yan X Y, Zuo C L, et al.Evolution and brassinosteroid response analysis of BZR1 gene family in soybean[J]. Journal of Hebei Normal University (Natural Science), 2023, 47(6): 620-627.
[34] Kuijt S J H, Lamers G E M, Rueb S, et al. Different subcellular localization and trafficking properties of KNOX class 1 homeodomain proteins from rice[J]. Plant Molecular Biology, 2004, 55: 781-796.
[35] Otani Y, Tomonaga Y, Tokushige K, et al.Expression profiles of four BES1/BZR1 homologous genes encoding bHLH transcription factors in Arabidopsis[J]. Journal of Pesticide Science, 2020, 45(2): 95-104.
[36] 左春柳. 芹菜BZR1转录因子基因鉴定与功能研究[D]. 唐山: 华北理工大学, 2023.
Zuo C L.Identification and function analysis of BZR1 gene family in celery [D]. Tangshan: North China University of Science and Technology, 2023.
[37] 冯文奇, 孙福艾, 丁磊, 等. 玉米转录因子ZmBES1/BZR1-7基因克隆及功能分析[J]. 核农学报, 2020, 34(1): 17-25.
Feng W Q, Sun F A, Ding L, et al.Cloning and functional analysis of maize transcription factor ZmBES1/BZR1-7[J]. Journal of Nuclear Agricultural Sciences, 2020, 34(1): 17-25.
[38] Li R, Zhang B, Li T, et al.Identification and characterization of the BZR transcriptionfactor genes family in potato (Solanum tuberosum L.) and their expression profiles in response to abiotic stresses[J]. Plants, 2024, 13(3): 407. doi: 10.3390/plants13030407.
[39] Chen J, Nolan T M, Ye H, et al.Arabidopsis WRKY46, WRKY54, and WRKY70 transcription factors are involved in brassinosteroid-regulated plant growth and drought responses[J]. Plant Cell, 2017, 29(6): 1425-1439.
[40] Cui X Y, Gao Y, Guo J, et al.BES/BZR transcription factor TaBZR2 positively regulates drought responses by activation of TaGST1[J]. Plant Physiology, 2019, 180(1): 605-620.
[41] Sahni S, Prasad B, Liu Q, et al.Overexpression of the brassinosteroid biosynthetic gene DWF4 in Brassica napus simultaneously increases seed yield and stress tolerance[J]. Scientific Reports, 2016, 6: 28298. doi: 10.1038/srep28298.
[42] 魏鑫, 倪虹, 张会慧, 等. 外源脱落酸和油菜素内酯对干旱胁迫下大豆幼苗抗旱性的影响[J]. 中国油料作物学报, 2016, 38(5): 605-610.
Wei X, Ni H, Zhang H H, et al.Effects of exogenous abscisic acid and brassinolide on drought resistance of soybean seedlings[J]. Chinese Journal of Oil Crop Sciences, 2016, 38(5): 605-610.
[43] 程鸿燕, 郭昱, 马芳芳, 等. 谷子NCED基因家族鉴定及其干旱胁迫响应表达模式分析[J]. 江苏农业科学, 2019, 47(1): 40-44.
Cheng H Y, Guo Y, Ma F F, et al.Identification of NCED gene family and analysis of their expression patterns in response to drought stress in Setaria italica[J]. Jiangsu Agricultural Sciences, 2019, 47(1): 40-44.

Cloning of BZR1 Gene Family in Tea Plants and Molecular Mechanism Study of CsBZR1-5 Response to Drought Stress

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