Young shoots purple-related differentially expressed genes of tea plant have been found in purple and green shoots line of Longjing Qunti by cDNA microarray technique. There were 43 differentially expressed genes relating to purple shoots, among them 17 were up-regulated and 26 were down-regulated genes. Three differentially expressed genes and two no change genes were selected to verify the results of cDNA microarray hybridizations. The result of real time RT-PCR analysis was consistent with that of cDNA microarray. Then all of the differentially expressed genes were annotated functionally by Blast X search against the NCBI non-redundant protein databases. The molecular function of differentially expressed genes mainly included energy metabolism, secondary metabolism and transcriptional regulation. Especially two genes and two transcription factors involved in the biosynthesis pathway of anthocyanidin were selected from the results of cDNA microarray hybridizations. The phenylalanine ammonia lyase gene, MYB protein, WD40-like protein, and anthocyanidin reductase gene showed down-regulated 3.44, 2.25, 2.54 folds and up-regulated 2.09 folds, respectively. This study establishes a basis for further understanding the purple mechanism of tea young shoots and related gene cloning.
MA Chun-lei
,
YAO Ming-zhe
,
WANG Xin-chao
,
JIN Ji-qiang
,
CHEN Liang
. Young Shoot Purple-Related Gene Screening in Tea Plant (Camellia sinensis) by Using cDNA Microarray[J]. Journal of Tea Science, 2011
, 31(1)
: 59
-65
.
DOI: 10.13305/j.cnki.jts.2011.01.010
[1] 梁名志, 夏涛. 特种紫茶降压活性物质初探[J]. 云南农业大学学报, 2003, 18(4): 378-381.
[2] 张泽岑, 王能彬. 光质对茶树花青素含量的影响[J]. 四川农业大学学报, 2002, 20(4): 337-382.
[3] Xie DY, Sharma SB, Paiva NL, et al. Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis[J]. Science, 2003, 299(5605): 396-399.
[4] Negishi H, Xu J W, Ikeda K.Black and green tea polyphenols attenuate blood pressure increases in stroke-prone spontaneously hypertensive rats[J]. J Nutr, 2004, 134(1): 38-42.
[5] Cabrera C, Artacho R, Gimenez R.Beneficial effects of green tea-a review[J]. J Am Coll Nutr, 2006, 25(2): 79-99.
[6] Zheng G, Sayama K, Okubo T.Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine in mice[J]. In Vivo, 2004, 18(1): 55-62.
[7] Holton T A, Cornish E C.Genetics and biochemistry of anthocyanin biosynthesis[J]. Plant Cell, 1995, 7(7): 1071-1083.
[8] Sharma SB, Dixon RA.Metabolic engineering of proanthocyanidins by ectopic expression of transcription factors in Arabidopsis thaliana[J]. Plant J, 2005, 44(1): 62-75.
[9] Lepiniec L, Debeaujon I, Routaboul JM, et al. Genetics and biochemistry of seed flavonoids[J]. Annu Rev Plant Biol, 2006, 57: 405-430.
[10] 赵文军, 张迪, 马丽娟, 等. 原花青素的生物合成途径、功能基因和代谢工程[J]. 植物生理学通讯, 2009, 45(5): 509-519.
[11] 夏涛, 高丽萍. 类黄酮及茶儿茶素生物合成途径及其调控研究进展[J]. 中国农业科学, 2009, 42(8): 2899-2908.
[12] 赵丽萍, 高其康, 陈亮, 等. 茶树基因芯片的研制和初步应用[J]. 茶叶科学, 2006, 26(3): 166-170.
[13] 张亚丽, 乔小燕, 陈亮. 茶树ACC 氧化酶基因全长cDNA 的克隆与表达分析[J]. 茶叶科学, 2008, 28(6): 459-467.
[14] Yang YH, Dudoit S, Luu P, et al. Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation[J]. Nucleic Acids Res, 2002, 30(4): e15.
[15] Tusher VG, Tibshirani R, Chu G.Significance analysis of microarrays applied to the ionizing radiation response[J]. Proc Natl Acad Sci USA, 2001, 98(9): 5116-5121.
[16] Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J]. Methods, 2001, 25(4): 402-408.
[17] Andreas R, Alfred Z, Dieter M, et al. The FunCat: a functional annotation scheme for systematic classification of proteins from whole genomes[J]. Nucleic Acids Res, 2004, 32(18): 5539-5545.
[18] Ramsay NA, Glover BJ.MYB-bHLH-WD40 protein complex and the evolution of cellular diversity[J]. Trends plant sci, 2005, 10(2): 63-70.
[19] Mano H, Ogasawara F, Sato K, et al. Isolation of a regulatory gene of anthocyanin biosynthesis in tuberous roots of purple-fleshed sweet potato[J]. Plant Physiol, 2007, 143(3): 1252-1268.
浙公网安备 33019902000101号