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Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (1): 59-65.doi: 10.13305/j.cnki.jts.2011.01.010

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Young Shoot Purple-Related Gene Screening in Tea Plant (Camellia sinensis) by Using cDNA Microarray

MA Chun-lei, YAO Ming-zhe, WANG Xin-chao, JIN Ji-qiang, CHEN Liang*   

  1. Research Center for Tea Germplasm and Improvement, Tea Research Institute of the Chinese Academy of Agricultural Sciences/National Center for Tea Improvement, Hangzhou 310008, China
  • Received:2010-04-17 Revised:2010-11-20 Online:2011-02-15 Published:2019-09-06

Abstract: Young shoots purple-related differentially expressed genes of tea plant have been found in purple and green shoots line of Longjing Qunti by cDNA microarray technique. There were 43 differentially expressed genes relating to purple shoots, among them 17 were up-regulated and 26 were down-regulated genes. Three differentially expressed genes and two no change genes were selected to verify the results of cDNA microarray hybridizations. The result of real time RT-PCR analysis was consistent with that of cDNA microarray. Then all of the differentially expressed genes were annotated functionally by Blast X search against the NCBI non-redundant protein databases. The molecular function of differentially expressed genes mainly included energy metabolism, secondary metabolism and transcriptional regulation. Especially two genes and two transcription factors involved in the biosynthesis pathway of anthocyanidin were selected from the results of cDNA microarray hybridizations. The phenylalanine ammonia lyase gene, MYB protein, WD40-like protein, and anthocyanidin reductase gene showed down-regulated 3.44, 2.25, 2.54 folds and up-regulated 2.09 folds, respectively. This study establishes a basis for further understanding the purple mechanism of tea young shoots and related gene cloning.

Key words: tea plant (Camellia sinensis), cDNA microarray, purple shoots, differentially expressed genes

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