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Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (1): 53-58.doi: 10.13305/j.cnki.jts.2011.01.009

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Cloning and Expression Analysis of Stress-resistant ERF Genes from Tea Plant [Camellia sinensis (L.) O. Kuntze]

CHEN Lin-bo1,2, FANG Chao1, WANG Yu1, LI Ye-yun1, JIANG Chang-jun1*, LIANG Ming-zhi2   

  1. 1. Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Education,Anhui Agricultural University, Hefei 230036, China;
    2. Tea Research Institute, Yunnan Academy of Agricultural Science, Menghai 666201, China
  • Received:2010-07-26 Revised:2010-11-27 Online:2011-02-15 Published:2019-09-06

Abstract: For A TDF, which has been gained from genes expressed differentially in tea plant under cold stress using cDNA-AFLP, containing a complete coding sequence cDNA was cloned by RACE,named CsERF, and contains an ORF, which encodes a polypeptide of 212 amino acids including a conserved AP2 domains. Sequence alignment showed that CsERF protein shared high identity with other plants. qRT-PCR analysis showed that ERF gene was up-regulated by cold, ethylene, dehydration, NaCl and the maximum expression were 121.1, 22.6, 2.6, 2.2 times higher than before treatment, respectively. For different tea organs, transcriptional level of the ERF gene was the highest in mature leaves, the next was in bud, the third was in root and stem, and the lowest in flower and seed. It deduced that ERF gene was very important for tea plant in response to abiotic stress and was strictly controlled in expression of different organs.

Key words: Camellia sinensis, ERF Transcription Factor, gene clone, qRT-PCR, expression analysis

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