Anthocyanin Accumulation and Expression of Synthesis-related Genes in Leaves of Different Developmental Stages in Camellia sinensis cv. Zijuan

JIANG Huibing; SUN Yunnan; LI Mei; DAI Weidong; SONG Weixi; TIAN Yiping; XIA Lifei; CHEN Linbo

doi:10.13305/j.cnki.jts.2018.02.008

PDF(987 KB)

Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (2) : 174-182. DOI: 10.13305/j.cnki.jts.2018.02.008

Anthocyanin Accumulation and Expression of Synthesis-related Genes in Leaves of Different Developmental Stages in Camellia sinensis cv. Zijuan

JIANG Huibing¹, SUN Yunnan¹, LI Mei¹, DAI Weidong², SONG Weixi¹, TIAN Yiping¹, XIA Lifei¹, CHEN Linbo^1,*

Author information +

History +

Abstract

In order to explore the regulatory factors and structural genes of anthocyanin biosynthesis in leaves of different developmental stages in C. sinensis cv. Zijuan, anthocyanin contents and compositions were analyzed by high performance liquid chromatography-mass, and the expression of synthesis-related genes were tested by RNA-sequencing and digital gene expression (DGE) profiling technology. HPLC-MS analysis showed that the anthocyanin content was consistent with color changes, increased firstly and then decreased with the leaf positions of Zijuan, with the highest anthocyanin content in the second leaf (9.87βmg·g^-1) and the lowest content in mature leaf (0.11βmg·g^-1). DGE determination results showed that the expression levels of PAL gene were relatively high in bud, second leaf and open surface leaf, but low in mature leaf. C4H, 4CL, CHS, CHI, F3H, F3'H, F3'5'H and ANS showed a pattern of declining expression as PAL in different leaf positions of Zijuan. FLS was firstly up-regulated in the second leaf and then declined in the mature leaf, which was consistent to the anthocyanin changes. DFR showed no clear expression pattern. GT and ACT exhibited similar expression patterns, which were up-regulated in the second, open surface and mature leaves. ANR and LAR showed similar expression patterns, which were high in bud, second and open surface leaves, but low in mature leaf. The gene expression of bHLH, MYB and WDR showed different expression patterns in different leaf positons of Zijuan. These results suggested that the temporal expression specificities of structural and regulatory genes may significantly affect the accumulation of anthocyanin in Zijuan.

Key words

anthocyanin / differentially expressed genes / digital expression patterns / tea cultivar

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

JIANG Huibing, SUN Yunnan, LI Mei, DAI Weidong, SONG Weixi, TIAN Yiping, XIA Lifei, CHEN Linbo. Anthocyanin Accumulation and Expression of Synthesis-related Genes in Leaves of Different Developmental Stages in Camellia sinensis cv. Zijuan[J]. Journal of Tea Science. 2018, 38(2): 174-182 https://doi.org/10.13305/j.cnki.jts.2018.02.008

References

[1] Jiang LH, Shen XJ, Shoji T, et al.Characterization and activity of anthocyanins in Zijuan tea (Camellia sinensis var. kitamura)[J]. Journal of Agricultural & Food Chemistry, 2013, 61(13): 3306-3310.
[2] 赵瑜, 周家春, 张靖伟, 等. 紫娟茶提取物对血管紧张素转换酶、α-淀粉酶和胰脂肪酶的体外抑制作用[J]. 食品工业科技, 2017, 38(19): 11-20.
[3] 吴华玲, 何玉媚, 李家贤, 等. 11个红紫顶芽茶树新品系的顶芽叶特性和生化成分研究[J]. 植物遗传资源学报, 2012, 13(1): 42-47.
[4] 周琼琼, 孙威江. 茶树顶芽叶紫化的生理生化分析及其关键酶基因的表达[J]. 生物技术通报, 2015, 31(1): 86-91.
[5] 马春雷, 姚明哲, 王新超, 等. 利用基因芯片筛选茶树芽叶紫化相关基因[J]. 茶叶科学, 2011, 31(1): 59-65.
[6] Rani A, Singh K, Ahuja PS, et al.Molecular regulation of catechins biosynthesis in tea [Camellia sinensis (L.) O. Kuntze][J]. Gene, 2012, 495(2): 205-210.
[7] 陈林波, 夏丽飞, 孙云南, 等. 特异茶树品种“紫娟”叶色转变的基因表达差异分析[J]. 茶叶科学, 2012, 32(1): 59-65.
[8] 马春雷, 姚明哲, 王新超, 等. 茶树2个MYB转录因子基因的克隆及表达分析[J]. 林业科学, 2012, 48(3): 31-37
[9] 贡年娣, 郭丽丽, 王弘雪, 等. 茶树两个MYB转录因子基因的克隆及功能验证[J]. 茶叶科学, 2014, 34(1): 36-36.
[10] 陈伟, 吕晓杰, 宋晓敏, 等. ‘紫鹃’茶树紫叶和绿叶差异表达蛋白分析[J]. 福建农业学报, 2016, 31(3): 230-235.
[11] 唐秀华, 孙威江, 唐琴. 紫化茶树生理生化及其花青素调控机理研究进展[J]. 天然产物研究与开发, 2017, 29(6): 1077-1083.
[12] 陈林波, 夏丽飞, 周萌, 等. 基于RNA-Seq技术的“紫娟”茶树转绿组分析[J]. 分子植物育种, 2015, 13(10): 2250-2255.
[13] 李智, 王日为, 张丽霞, 等. 茶树紫色芽叶花青苷组分分析及结构推测[J]. 茶叶科学, 2014, 34(3): 279-287.
[14] 史成颖, 宛晓春, 江昌俊, 等. 提取高质量茶树总RNA的方法研究[J]. 安徽农业大学学报, 2007, 34(3): 360-363.
[15] 解东超, 戴伟东, 李朋亮, 等. 基于LC-MS的紫娟烘青绿茶加工过程中花青素变化规律研究[J]. 茶叶科学, 2016, 36(6): 603-612.
[16] Singh K, Kumar S, Rani A, et al.Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea[J]. Funct Integr Genomic, 2009, 9(1): 125-134.
[17] 周天山, 王新超, 余有本, 等. 紫顶芽茶树类黄酮生物合成关键酶基因表达与总儿茶素、花青素含量相关性分析[J]. 作物学报, 2016, 42(4): 525-531.
[18] 韩科厅, 赵莉, 唐杏姣, 等. 菊花花青素苷合成关键基因表达与花色表型的关系[J]. 园艺学报, 2012, 39(3): 516-524.
[19] Davies KM, Schwinn KE, Deroles SC, et al.Enhancing anthocyanin production by altering competition for substrate between flavonol synthase and dihydroflavonol 4-reductase[J]. Euphytica, 2003, 131(3): 259-268.
[20] Tsuda S, Fukui Y, Nakamura N, et al.Flower color modification of Petunia hybrida commercial varieties by metabolic engineering[J]. Plant Biotechnology, 2004, 21(5): 377-386.
[21] 戴思兰, 洪艳. 基于花青素苷合成和呈色机理的观赏植物花色改良分子育种[J]. 中国农业科学, 2016, 49(3): 529-542.
[22] 王晓帆, 田艳维, 王云生, 等. 茶树类黄酮3-O-葡萄糖基转移酶基因的克隆和表达分析[J]. 茶叶科学, 2012, 32(5): 411-418.
[23] 祝志欣, 鲁迎青. 花青素代谢途径与植物颜色变异[J]. 植物学报, 2016, 9(1): 107-119.
[24] Liao L, Vimolmangkang S, Wei C, et al.Molecular characterization of genes encoding leucoanthocyanidin reductase involved in proanthocyanidin biosynthesis in apple[J]. Frontiers in Plant Science, 2015, 10(6): 234.
[25] Wada T, Kunihiro A, Tominagawada R. Arabidopsis CAPRICE (MYB) and GLABRA3 (bHLH) control tomato (Solanum lycopersicum) anthocyanin biosynthesis[J]. PLOS ONE, 2014, 9(9): e109093.
[26] Albert NW, Davies KM, Lewis DH, et al.A conserved network of transcriptional activators and repressors regulates anthocyanin pigmentation in eudicots[J]. Plant Cell, 2014, 26(3): 962-980.
[27] Taylor LP, Grotewold E.Flavonoids as developmental regulators[J]. Curr Opin Plant Biol, 2005, 8(3): 317-323.
[28] Verdonk JC, Haring MA, Van-Tunen AJ, et al.ODORANT1 regulates fragrance biosynthesis in petunia flowers[J]. Plant Cell, 2005, 17(5): 1612-1624.
[29] Fornale S, Lopez E, Salazar-Henao JE, et al.AtMYB7, a new player in the regulation of UV-Sunscreens in Arabidopsis thaliana[J]. Plant Cell Physiology, 2014, 55(3): 507-516.
[30] Jin H, Cominelli E, Bailey P, et al.Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis[J]. The Embo Journal, 2000, 19(22): 6150-6161