红紫芽茶花青素合成关键酶活性与重要酚类物质相关性研究

doi:10.13305/j.cnki.jts.20201109.001

Abstract

Abstract: In this study, four purple tea cultivars (strains) namely Hongye 1, Hongye 2, Danfei from Guangdong and Zijuan from Yunnan were used as the testing materials. Yinghong 9 with green buds was used as the control. The activity changing patterns of the key enzymes involved in anthocyanin synthesis were studied by the enzyme activities analysis, which revealed the relationship between the enzyme activities and biochemical components in tea. The results show that the activity of flavonoid-3-O-glycosyltransferase (UFGT), a key enzyme involved in anthocyanin synthesis, was positively correlated with the total amount of tea polyphenols and the anthocyanin contents in different samples in the same season. However, the activities of phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), flavonoid-3-hydroxylase (F3H), dihydroflavonol-4-reductase (DFR), flavonol synthase (FLS), anthocyanin synthase (ANS) and anthocyanin reductase (ANR) were not significantly correlated with the anthocyanin contents. There was a significant and positive correlation between catechin (C) content and PAL enzyme activity in spring. Epicatechin gallate (ECG) content and DFR enzyme activity showed the same result. In addition, there was no significant correlation between the activities of CHS, F3H, ANS and ANR enzymes and polyphenols contents in different cultivars (strains) in the same season.

Key words: purple tea, anthocyanin, enzyme, polyphenols

CLC Number:

S571.1

CAO Bingbing, WANG Qiushuang, QIN Dandan, FU Donghe, FANG Kaixing, JIANG Xiaohui, LI Hongjian, WANG Qing, PAN Chendong, LI Bo, WU Hualing. Study on the Correlation between the Activities of Key Enzymes Involving in Anthocyanin Synthesis and the Contents of Important Polyphenols in Purple Tea[J]. Journal of Tea Science, 2020, 40(6): 724-738.

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Study on the Correlation between the Activities of Key Enzymes Involving in Anthocyanin Synthesis and the Contents of Important Polyphenols in Purple Tea

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