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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (3): 377-388.doi: 10.13305/j.cnki.jts.2023.03.005

• Research Paper • Previous Articles     Next Articles

Preparation of a Cross-linked Polyphenol Oxidase Aggregate Based on Catechins and Its Efficient Catalytic Synthesis of Theaflavin-3,3ʹ-digalate

ZHOU Jinghui1,2,3,4,5, LIU Changwei1,2,3,4, ZHANG Sheng1,2,3,4, XU Gang5, XU Wei6, HUANG Jian′an1,2,3,4, LIU Zhonghua1,2,3,4,*   

  1. 1. Key Laboratory of Tea Science of Ministry of Education, Hunan agricultural university, Changsha 410128, China;
    2. National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    3. Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    4. Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops, Ministry of Agriculture and Rural Affairs of China, Changsha 410128, China;
    5. National and local joint Engineering Research Center of Enzyme Technology for Pharmaceutical Industry, Hunan Flag Bio-technology Co., Ltd., Changsha 410100, China;
    6. Tea Refining and Innovation Key Laboratory of Sichuan Province, Yibin 644000, China
  • Received:2023-02-14 Revised:2023-04-06 Online:2023-06-15 Published:2023-06-29

Abstract: In order to prepare theaflavin more efficiently and economically, catechins were used as the cross-linking reagent in this study, and a polyphenol oxidase (tyrosinase) crossed-linked aggregate derived from Bacillus megaterium was prepared and used for the synthesis of theaflavin-3,3ʹ-digalate. Through optimization of enzyme cross-linking parameters and the catalytic performance before and after cross-linking, the results show that the optimal enzyme activity recovery rate of cross-linked enzyme (200 U·mL-1) could be obtained at pH 4.0, EGCG 0.5 mg·mL-1 and cross-linking time 50 min. Compared with free enzymes, cross-linked enzymes showed better catalytic performance (thermal stability, organic solvent tolerance, substrate tolerance). When the cross-linked enzyme was used to synthesize theaflavin-3,3′-digallate, the concentration of the product could reach 800 μg·mL-1, and the cross-linked enzyme could be reused for at least three batches. This method could significantly reduce the application cost of theaflavin, which has potential industrial application value.

Key words: enzyme cross-linked aggregates, tyrosinase, preparation, theaflavins

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