Research on the Antihyperlipdemic Activity of Theaflavins, Catechins and Saponius Mixture Preparations

WANG Zhijun; ZHANG Cong; TAN Rong; KONG Junhao; GE Qingfeng; YANG Xiufang

doi:10.13305/j.cnki.jts.2014.04.013

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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (4) : 401-407. DOI: 10.13305/j.cnki.jts.2014.04.013

Research on the Antihyperlipdemic Activity of Theaflavins, Catechins and Saponius Mixture Preparations

ZHANG Cong^1,2,3, TAN Rong^2,3,*, KONG Junhao^2,3, GE Qingfeng¹, YANG Xiufang^2,3, WANG Zhijun¹

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Abstract

The antihyperlipdemic activity in vitro of theaflavins(TFs), catechins(Cs) and saponius(SPs) were assessed in lipid clearance rate in the model of HepG2 steatosis by using the admini stration with 1 mmol·L^-1 oleic acid(OA) in 24 h. Based on the cell viability test(MTT value), lipid clearance was used to evaluate the effect of TFs, Cs and SPs on HepG2 steatosis. The response surface methodology based on a Box-Behnken experimental design was used to optimize the density of the three extractions for combined lipid-lowering in vitro. Results indicated that TFs, Cs and SPs showed a significant inhibitory effect on TG accumulation in HepG2 cells. The ability of lipid-lowering was significant promoted when the three extractions acted on HepG2 at the same time. TFs, Cs and SPs could showed the prevention and curing effect of hyperlipidemia.

Key words

in vitro / antihyperlipdemic / catechins / saponius / theaflavins

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ZHANG Cong, TAN Rong, KONG Junhao, GE Qingfeng, YANG Xiufang, WANG Zhijun. Research on the Antihyperlipdemic Activity of Theaflavins, Catechins and Saponius Mixture Preparations[J]. Journal of Tea Science. 2014, 34(4): 401-407 https://doi.org/10.13305/j.cnki.jts.2014.04.013

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