Bioinformatic Analysis of MYB Transcription Factors Involved in Catechins Biosynthesis in Tea Plant (Camellia sinensis)

ZHANG Yue; HU Yunfei; WANG Shumao; KE Zixing; LIN Jinke

doi:10.13305/j.cnki.jts.2018.02.007

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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (2) : 162-173. DOI: 10.13305/j.cnki.jts.2018.02.007

Bioinformatic Analysis of MYB Transcription Factors Involved in Catechins Biosynthesis in Tea Plant (Camellia sinensis)

ZHANG Yue, HU Yunfei, WANG Shumao, KE Zixing, LIN Jinke^*

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Abstract

Six MYB transcription factors involved in the biosynthesis of catechins were obtained from the tea plant (Camellia sinensis) transcriptome database. Bioinformatic analysis including the physicochemical properties and structural functions of proteins, nuclear localization signal (NLS), protein conserved domain (PCD) and phylogenetic tree analysis were performed. The results demonstrated that the 6 MYB proteins involved in the biosynthesis of catechins belong to hydrophilic non-secretory proteins and are predicted to be located in the chloroplasts, mitochondria and nucleus. The main spatial structures are α-helixes and β-turns. Except the comp159173_c0, the other 5 genes belong to SANT super-family group based on the PCD analysis. The phylogenetic tree analysis shows that 6 MYB proteins are classified into 4 groups, indicating a distant relationship between them. Differential gene expression analysis further confirmed the close correlation between the 6 transcription factors and the catechin biosynthesis.

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bioinformatics / catechins / MYB transcription factors

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ZHANG Yue, HU Yunfei, WANG Shumao, KE Zixing, LIN Jinke. Bioinformatic Analysis of MYB Transcription Factors Involved in Catechins Biosynthesis in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2018, 38(2): 162-173 https://doi.org/10.13305/j.cnki.jts.2018.02.007

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