茶树果胶乙酰酯酶家族成员的鉴定和生物信息学分析

doi:10.13305/j.cnki.jts.2019.05.003

Abstract

Abstract: Tea plant is a fluoride hyperaccumulator with high fluoride in leaves, which has many health benefits to human body. Tea pectin acetylesterase (PAE) may be related to fluoride accumulation and detoxification by regulating pectin deacytelation. However, few reports on tea PAEs were available. In the study, the identification and analysis of characteristic, evolutionary and subcellular localization of CsPAEs were performed by bioinformatics method based on tea genome database and three next-generation sequencing data of Camellia sinensis var. sinensis cv ‘Shuchazao’. The results indicate that 12 CsPAEs belonged to five members, including PAE5, PAE8, PAE9, PAE10 and PAE12. Nine conserved motifs including CLDG, PxYH, GGGWC, GS, NWN, rYCDg, GCSAG, NaAYDSWQ and HCQ were found in CsPAEs; CsPAEs were more closely related to PAEs of Vitis vinifera and Theobroma cacao according to evolutionary relationship; Amino acid length, molecular weight and theoretical isoelectric point of 12 CsPAEs varies from 326-515, 36.7-56.9βkDa and 5.1-8.9, respectively. Except that CsPAE5 and CsPAE5-1 were predicated to be localized in the mitochondrion, and CsPAE10-1 might be present in cellular martix. The rest CsPAEs were predicated to be localized in the cell wall. CsPAE5, CsPAE5-1, CsPAE12 and CsPAE12-1 were transmembrane proteins. The results provided a foundation for functional analysis of CsPAEs involved in fluoride accumulation and detoxification.

Key words: tea plant (Camellia sinensis), pectin acetylesterase, identification, bioinformatics analysis

CLC Number:

LIU Yanli, MA Linlong, CAO Dan, JIN Xiaofang, FENG Lin, GONG Ziming. Identification and Bioinformatic Analysis of Pectin Acetylesterases from Tea Plant[J]. Journal of Tea Science, 2019, 39(5): 521-529.

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Identification and Bioinformatic Analysis of Pectin Acetylesterases from Tea Plant

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