Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (4): 431-439.doi: 10.13305/j.cnki.jts.2019.04.008

Previous Articles     Next Articles

Isolation, Screening and Identification of A Strain of Trichoderma Antagonizing Tea Anthracnose

ZHAO Xingli1, ZHANG Jinfeng2, ZHOU Yufeng1,*, ZHAO Dailin3, ZHANG Li4, ZHOU Luona1, TAO Gang3,*   

  1. 1. Guizhou Academy of Agricultural Sciences key laboatory of agricultural biotechnology, Guiyang 550006, China;
    2. Guizhou Academy of Agricultural Sciences tea research institute, Guiyang 550006, China;
    3. Guizhou Academy of Agricultural Sciences institute of Plant Protection, Guiyang 550006, China;
    4. Guizhou Academy of Agricultural Sciences grass industry research institute, Guiyang 550006, China
  • Received:2018-12-06 Online:2019-08-15 Published:2019-08-19

Abstract: To obtain antagonistic Trichoderma for biocontrol of tea anthracnose, the twenty-three Trichoderma strains were isolated from Camellia sinensis rhizosphere-soil by taking gradient dilution. Among them, the strain LS17110205 against tea anthracnose was screened by using dual-culture and inhibition zone assay. The results show that the inhibitory rate of LS17110205 against tea anthracnose was up to 76.96% and a large number of white hyphae and green spores were produced on the colony of tea anthracnose, which caused the tea anthracnose colony to shrink and became dark. The fermentation liquid of LS17110205 was effective against pathogen hyphae, and the inhibitory rate was 70.08%. The results show that the fermentation of the strain LS17110205 caused the tea anthracnose mycelia to shrink on the surface by scanning electron microscope analysis. LS17110205 was identified as Trichoderma asperelloides on the basis of the morphological characteristics and phylogenetic tree. The results provided theoretical basis in biocontrol of tea anthracnose.

Key words: rhizosphere-soil, inhibitory rate, tea anthracnose, identification, Trichoderma spp

CLC Number: