Pathogenicity and Fungicide Sensitivity of Colletotrichum camelliae from Tea Plant (Camellia sinensis)

CHENG Kaixin; YANG Kaixin; DENG Yayuan; LI Xin; LIU Enbei; WANG Yuchun; L&#x000dc; Wuyun

doi:10.13305/j.cnki.jts.2023.01.004

PDF(4410 KB)

Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (1) : 55-66. DOI: 10.13305/j.cnki.jts.2023.01.004

Research Paper

Pathogenicity and Fungicide Sensitivity of Colletotrichum camelliae from Tea Plant (Camellia sinensis)

CHENG Kaixin¹, YANG Kaixin¹, DENG Yayuan¹, LI Xin¹, LIU Enbei¹, WANG Yuchun^1,2,*, LÜ Wuyun^1,*

Author information +

History +

Abstract

Colletotrichum camelliae is the dominant species causing foliar diseases of tea plants (Camellia sinensis). In this study, 65 isolates of C. camelliae were obtained from diseased leaves of tea plants in the 13 main tea-producing provinces of China. Phylogenetic analysis based on the multigene sequences was performed on all isolates, combined with their characteristics of colony and conidia, showed that all isolates clustered with C. camelliae. Pathogenicity analysis performed on detached leaves showed that all isolates of C. camelliae could infect the tea leaves, but the virulence of the isolates displayed a significant difference. The pathogenicity of 12 isolates, including YCW1180, YCW1331, YCW1382, YCW1387, YCW1419, YCW1443, YCW1451, YCW1453, YCW1454, YCW1461, YCW1613 and YCW2134, was significantly stronger than that of other isolates. By contrast, the pathogenicity of YCW1378 isolate was the weakest. The sensitivity of 65 isolates to 25% pyraclostrobin was determined by analyzing the inhibition rate of mycelial growth. The results show that the inhibition rate of pyraclostrobin on mycelial growth of YCW1436 isolate was only 36.00%, showing the worst antimicrobial effect. However, the inhibition rate of mycelial growth of most isolates was higher than 70.00%, which suggests that most isolates were highly sensitive to 25% pyraclostrobin. Microscopic observation shows that this agent mainly affected the conidial germination of C. camelliae. Overall, this study provided a solid foundation for the effective control of the diseases.

Key words

Colletotrichum camelliae / fungicide sensitivity / pathogenicity / strain identification / tea plants

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

CHENG Kaixin, YANG Kaixin, DENG Yayuan, LI Xin, LIU Enbei, WANG Yuchun, LÜ Wuyun. Pathogenicity and Fungicide Sensitivity of Colletotrichum camelliae from Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2023, 43(1): 55-66 https://doi.org/10.13305/j.cnki.jts.2023.01.004

References

[1] Wang X, Feng H, Chang Y, et al.Population sequencing enhances understanding of tea plant evolution[J]. Nature Communications, 2020, 11: 4447. doi: 10.1038/s41467-020-
18228-8.
[2] Wang Y, Yao Z, Pan Z, et al.Tea-planted soils as global hotspots for N₂O emissions from croplands[J]. Environmental Research Letters, 2020, 15: 104018. doi: 10.1088/1748-9326/aba5b2.
[3] He S N, An T, A R, et al. Validation of reliable reference genes for RT-qPCR studies of target gene expression in Colletotrichum camelliae during spore germination and mycelial growth and interaction with host plants[J]. Frontiers in Microbiology, 2019, 10: 2055. doi: 10.3389/fmicb.2019.02055.
[4] Xiong F, Wang Y, Lu Q, et al.Lifestyle characteristics and gene expression analysis of Colletotrichum camelliae isolated from tea plant [Camellia sinensis (L.) O. Kuntze] based on transcriptome[J]. Biomolecules, 2020, 10(5): 782. doi: 10.3390/biom10050782.
[5] Lin S R, Lin Y H, Ariyawansa H A, et al.Analysis of the pathogenicity and phylogeny of Colletotrichum species associated with brown blight of tea (Camellia sinensis) in Taiwan[J]. Plant Disease, 2023, 107(1): 97-106.
[6] Liu F, Weir B S, Damm U, et al.Unravelling Colletotrichum species associated with Camellia: employing ApMat and GS loci to resolve species in the C. gloeosporioides complex[J]. Persoonia, 2015, 35: 63-86.
[7] 刘威, 袁丁, 尹鹏, 等. 茶树炭疽病的研究进展[J]. 热带农业科学, 2016, 36(11): 20-26.
Liu W, Yuan D, Yin P, et al.Research progress on anthracnose of tea plant[J]. Chinese Journal of Tropical Agriculture, 2016, 36(11): 20-26.
[8] Lu Q, Wang Y, Li N, et al.Differences in the characteristics and pathogenicity of Colletotrichum camelliae and C. fructicola isolated from the tea plant [Camellia sinensis (L.) O. Kuntze][J]. Frontiers in Microbiology, 2018, 9: 3060. doi: 10.3389/fmicb.2018.03060.
[9] Cannon P F, Damn U, Johnston P R, et al.Colletotrichum-current status and future directions[J]. Studies in Mycology, 2012, 73(1): 181-213.
[10] Orrock J M, Rathinasabapathi B, Richter B S.Anthracnose in U.S. tea: pathogen characterization and susceptibility among six tea accessions[J]. Plant Disease, 2020, 104(4): 1055-1059.
[11] 贡长怡, 刘姣姣, 邓强, 等. 茶树炭疽病病原菌鉴定及其致病性分析[J]. 园艺学报, 2022, 49(5): 1092-1101.
Gong C Y, Liu J J, Deng Q, et al.Identification and pathogenicity of Colletotrichum species causing anthracnose on Camellia sinensis[J]. Acta Horticulturae Sinica, 2022, 49(5): 1092-1101.
[12] Wang Y C, Hao X Y, Wang L, et al.Diverse Colletotrichum species cause anthracnose of tea plants (Camellia sinensis (L.) O. Kuntze) in China[J]. Scientific Reports, 2016, 6: 35287. doi: 10.1038/srep35287.
[13] 王国君, 熊建伟, 陈利军. 几种杀菌剂对信阳茶树炭疽病的防治效果评价[J]. 农药, 2016, 55(8): 604-606.
Wang G J, Xiong J W, Chen L J.Evaluation of control effects several fungicides on tea anthracnose in Xinyang[J]. Agrochemicals, 2016, 55(8): 604-606.
[14] He L F, Li X X, Gao Y Y, et al.Characterization and fungicide sensitivity of Colletotrichum spp. from different hosts in Shandong, China[J]. Plant Disease, 2019, 103(1): 34-43.
[15] 贡长怡. 茶树炭疽病菌的致病力分析及其对四种常规药剂的敏感性[D]. 合肥: 安徽农业大学, 2020.
Gong C Y.Pathogenicity of Colletotrichum species causing anthracnose of Camellia sinensis and their sensitivity to four conventional fungicides [D]. Hefei: Anhui Agricultural University, 2020.
[16] Cai L, Hyde K D, Taylor P W J, et al. A polyphasic approach for studying Colletotrichum[J]. Fungal Diversity, 2009, 39: 183-204.
[17] 王玉春, 刘守安, 卢秦华, 等. 中国茶树炭疽菌属病害研究进展及展望[J]. 植物保护学报, 2019, 46(5): 954-963.
Wang Y C, Liu S A, Lu Q H, et al.Research progress and prospects of Colletotrichum species causing tea plant diseases in China[J]. Journal of Plant Protection, 2019, 46(5): 954-963.
[18] Katoh K, Standley D M.MAFFT multiple sequence alignment software version 7: improvements in performance and usability[J]. Molecular Biology and Evolution, 2013, 30(4): 772-780.
[19] Tamura K, Peterson D, Peterson N, et al.MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 2011, 28(10): 2731-2739.
[20] Minh B Q, Schmidt H A, Chernomor O, et al.Corrigendum to: IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era[J]. Molecular Biology and Evolution, 2020, 37(8): 2461. doi: 10.1093/molbev/msaa131.
[21] Li J, Sun K, Ma Q P, et al.Colletotrichum gloeosporioides-contaminated tea infusion blocks lipids reduction and induces kidney damage in mice[J]. Frontiers in Microbiology, 2017, 8: 2089. doi: 10.3389/fmicb.2017.02089.
[22] Schoch C L, Ciufo S, Domrachev M, et al.NCBI Taxonomy: a comprehensive update on curation, resources and tools[J]. Database (Oxford), 2020: baaa062. doi: 10.1093/database/
baaa062.
[23] Costa L C, Nalin R S, Dias M A, et al.Different loci control resistance to different isolates of the same race of Colletotrichum lindemuthianum in common bean[J]. Theoretical and Applied Genetics, 2021, 134: 543-556.
[24] Onaga G, Wydra K, Koopmann B, et al.Population structure, pathogenicity, and mating type distribution of Magnaporthe oryzae isolates from East Africa[J]. Phytopathology, 2015, 105: 1137-1145.
[25] 朱名海. 我国南繁区水稻纹枯病菌和稻瘟病菌的遗传多样性研究[D]. 广州: 华南农业大学, 2016.
Zhu M H.Studies on the genetic diversity of Thanatephorus cucumeris and Magnaporthe oryzae from South China crop breeding area [D]. Guangzhou: South China Agricultural University, 2016.
[26] 许娟. 安徽小麦赤霉病菌生物学特征及遗传多样性研究[D]. 合肥: 安徽农业大学, 2012.
Xu J.Study on the biological characteristics and genetic diversity of Fusarium graminearum in Anhui [D]. Hefei: Anhui Agricultural University, 2012.
[27] Kanzaki H, Yoshida K, Saitoh H, et al.Arms race co-evolution of Magnaporthe oryzae AVR-Pik and rice Pik genes driven by their physical interactions[J]. The Plant Journal, 2012, 72(6): 894-907.
[28] Gao Y Y, Li X X, He L F, et al.Effect of application rate and timing on residual efficacy of pyraclostrobin in the control of pepper anthracnose[J]. Plant Disease, 2020, 104: 958-966.
[29] Turechek W W, Peres N A, Werner N A.Pre- and post-infection activity of pyraclostrobin for control of anthracnose fruit rot of strawberry caused by Colletotrichum acutatum[J]. Plant Disease, 2006, 90: 862-868.
[30] Acharya B, O’Quinn T N, Everman W, et al. Effectiveness of fungicides and their application timing for the management of sorghum foliar anthracnose in the mid-Atlantic United States[J]. Plant Disease, 2019, 103: 2804-2811.
[31] Li X X, Liu Y, He L F, et al.Fungicide formulations influence their control efficacy by mediating physicochemical properties of spay dilutions and their interaction with target leaves[J]. Journal of Agricultural Food Chemistry, 2020, 68(5): 1198-1206.
[32] 梁碧元, 陈方景. 8种杀菌剂防治茶树茶饼病的效果试验[J]. 中国园艺文摘, 2013, 29(9): 46-47.
Liang B Y, Chen F J.Control effect of eight fungicides on Exobasidium vexans[J]. Chinese Horticulture Abstracts, 2013, 29(9): 46-47.
[33] 吴庆丽, 秦刚, 李慧, 等. 助剂激健与杀菌剂混用对3种茶树病害的防效[J]. 中国植保导刊, 2020, 40(8): 69-71, 77.
Wu Q L, Qin G, Li H, et al.The effect of the mixture of adjuvant ‘Jijian’ and fungicides against three diseases on tea[J]. China Plant Protection, 2020, 40(8): 69-71, 77.