Early Identification of Nitrogen Absorption Efficiency in Tea Plants

SU Jingjing; RUAN Li; WANG Liyuan; WEI Kang; WU Liyun; BAI Peixian; CHENG Hao

doi:10.13305/j.cnki.jts.2020.05.002

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (5) : 576-587. DOI: 10.13305/j.cnki.jts.2020.05.002

Research Paper

Early Identification of Nitrogen Absorption Efficiency in Tea Plants

SU Jingjing^1,2, RUAN Li¹, WANG Liyuan¹, WEI Kang¹, WU Liyun¹, BAI Peixian^1,2, CHENG Hao^1,*

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Abstract

Nitrogen is one of the most important elements for tea plants, and it has been over supplied in tea gardens, which not only results in the waste of resources, but also causes a series of environmental problems. Therefore, to breed tea cultivars with high nitrogen efficiency, it is necessary to establish an early detection of nitrogen usage rates which could be developed for screening the strains of tea plants. This study analyzed the absorption and utilization of ammonia nitrogen and nitrate nitrogen in two tea cultivars Longjing 43 (LJ43) and Zhongcha 108 (ZC108) under different nitrogen levels. The ¹⁵N isotope labeling technology was applied to verify the feasibility and practicability of non-invasive micro-test technology (NMT) and real-time fluorescence quantitative (qRT-PCR) technology in early identification of nitrogen absorption and utilization abilities of tea lines. The purpose of this study was to establish an indoor early identification technology of nitrogen absorption efficiency for tea plants. The results show that the accuracy, stability and repeatability of ¹⁵N were 85.16%, 89.51% and 99.26% respectively. While the accuracy, stability and repeatability of NMT were 91.35%, 95.22% and 96.76% respectively. The two methods showed that tea plants had obvious ammonium preference. Moreover, the expressions of nitrate transporter genes CsNRT3.2 and CsNRT2.4 were up-regulated by nitrogen in both cultivars. Compared with ZC108, the expressions of CsNRT2.4 and CsNRT3.2 in LJ43 were higher, indicating that the response of LJ43 to external nitrogen applications was higher than that of ZC108. Finally, it was preliminarily summarized that the NMT technology could measure the instantaneous absorption rate of tea plants in a short time with little loss of experimental materials. It might also be applied for the early detection of the instantaneous nitrogen absorption rates of tea plants. Meanwhile, the results also show that the expressions of CsNRT2.4 and CsNRT3.2 could partly reflect the nitrogen absorption ability of tea plants. This study provided a basis to develop techniques in early identification of high nitrogen-efficient cultivars in tea plants.

Key words

¹⁵N isotope-labeled / nitrogen uptake rate / non-invasive micro-test technology (NMT) / qRT-PCR / tea plants

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SU Jingjing, RUAN Li, WANG Liyuan, WEI Kang, WU Liyun, BAI Peixian, CHENG Hao. Early Identification of Nitrogen Absorption Efficiency in Tea Plants[J]. Journal of Tea Science. 2020, 40(5): 576-587 https://doi.org/10.13305/j.cnki.jts.2020.05.002

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