Effects of Exogenous 5-aminolevulinic Acid on Photosynthetic and Physiological Characteristics of Tea Plants under Low Temperature Stress

YAN Fei; JIANG Wenhua; QU Dong; FU Jing; ZHAO Xuan

doi:10.13305/j.cnki.jts.2020.05.004

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (5) : 597-606. DOI: 10.13305/j.cnki.jts.2020.05.004

Effects of Exogenous 5-aminolevulinic Acid on Photosynthetic and Physiological Characteristics of Tea Plants under Low Temperature Stress

YAN Fei^1,2,3,4, JIANG Wenhua¹, QU Dong^1,2,3, FU Jing^1,2,3,4, ZHAO Xuan⁴

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Abstract

In order to explore the effects of 5-aminolevulinic acid (ALA) on photosynthetic and physiological characteristics of tea plants under natural low temperature stress in winter, we sprayed 0, 10, 30mg·L^-1 and 50 mg·L^-1 ALA on ‘Shaancha 1’ (low temperature tolerant cultivar) and ‘Jinmudan’ (low temperature sensitive cultivar). The results show that ALA of suitable concentration could improve net photosynthetic rate, stomatal conductance, intercellular CO₂ concentration, transpiration rate, maximum photochemical efficiency of PSⅡ and potential activity of PSⅡ. Moreover, ALA treatment could promote the accumulations of water extract, caffeine, free amino acids, catechins, theanine, soluble sugar and protein contents. Exogenous ALA could improve the photosynthetic capacity and tea quality under low temperature stress. ALA treatment (50 mgL^-1) could effectively improve the cold tolerance of ‘Shaancha 1’. While 10 mg·L^-1 and 30 mg·L^-1 ALA treatment had effects on the relief of low temperature stress in ‘Jinmudan’.

Key words

ALA / low temperature stress / photosynthetic characteristics / physiological characteristics / tea plants

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YAN Fei, JIANG Wenhua, QU Dong, FU Jing, ZHAO Xuan. Effects of Exogenous 5-aminolevulinic Acid on Photosynthetic and Physiological Characteristics of Tea Plants under Low Temperature Stress[J]. Journal of Tea Science. 2020, 40(5): 597-606 https://doi.org/10.13305/j.cnki.jts.2020.05.004

References

[1] 韩文炎, 李鑫, 颜鹏, 等. 茶园“倒春寒”防控技术[J]. 中国茶叶, 2018, 40(2): 9-12.
Han W Y, Li X, Yan P, et al.Prevention and control technology of ‘late spring cold’ in tea garden[J]. China Tea, 2018, 40(2): 9-12.
[2] 李智祥, 赵磊, 梁云龙, 等. 生物法合成5-氨基乙酰丙酸的研究进展[J]. 发酵科技通讯, 2017, 46(3): 178-182.
Li Z X, Zhao L, Liang Y L, et al.Advance on biosynthesis of 5-aminolevulinic acid[J]. Bulletin of Fermentation Science and Technology, 2017, 46(3): 178-182.
[3] Wu Y, Liao B W, Mohammed M D, et al.5-aminolevulinic acid (ALA) biosynthetic and metabolic pathways and its role in higher plants: a review[J]. Plant Growth Regulation, 2019, 87(2): 357-374.
[4] Hotta Y, Tananka T, Bingshan L, et al.Improvement of cold resistance in rice seedlings by 5-aminolevulinic acid[J]. Journal of Pesticide Science, 1998, 23(1): 29-33.
[5] 孙阳, 王燚, 孟瑶, 等. 外源5-氨基乙酰丙酸对低温胁迫下玉米幼苗生长及光合特性的影响[J]. 作物杂志, 2016(5): 87-93.
Sun Y, Wang Y, Meng Y, et al.Effects of exogenous ALA on growth and photosynthetic characteristics of maize seedlings under low temperature stress[J]. Crops, 2016(5): 87-93.
[6] Fan H F, Du C X, Guo S R.Effect of nitric oxide on proline metabolism in cucumber seedlings under salinity stress[J]. Journal of the American Society for Horticultural Science, 2012, 137(3): 127-133.
[7] Fan H F, Du C X, Guo S R.Nitric oxide enhances salt tolerance in cucumber seedlings by regulating free polyamine content[J]. Environmental and Experimental Botany, 2013, 86: 52-59.
[8] Fu J J, Sun Y F, Chu X T, et al.Exogenous 5-aminolevulenic acid promotes seed germination in Elymus nutans against oxidative damage induced by cold stress[J]. Plos One, 2014, 9(9): e107152. doi: 10.1371/journal.pone.0107152.
[9] 王宏信, 剧春晖, 李向林, 等. 外源5-氨基乙酰丙酸对干旱胁迫下降香黄檀幼苗生长、根系生理特性的影响[J]. 热带作物学报, 2017, 38(10): 1823-1829.
Wang H X, Ju C H, Li X L, et al.Effects of exogenous 5-aminolevulinic acid on growth and root physiological characteristics of Dalbergia odorifera T.chen under drought stress[J]. Chinese Journal of Tropical Crops, 2017, 38(10): 1823-1829.
[10] 孟长军, 杜喜春, 张旸. 5-氨基乙酰丙酸对喜树幼苗盐害缓解的生理机制研究[J]. 热带亚热带植物学报, 2019, 27(2): 164-170.
Meng C J, Du X C, Zhang Y.Study on physiological mechanism of alleviating effect of 5-aminolevulinic acid on Camptotheca acuminata seedlings under salt stress[J]. Journal of Tropical and Subtropical Botany, 2019, 27(2): 164-170.
[11] 李海燕, 毕文双, 王燚, 等. 外源ALA对低温胁迫下玉米幼苗根系生长及生理特性的影响[J]. 东北农业大学学报, 2019, 50(2): 9-17.
Li H Y, Bi W S, Wang Y, et al.Effect of exogenous ALA on root growth and physiological characteristics of maize seedlings under low temperature stress[J]. Journal of Northeast Agricultural University, 2019, 50(2): 9-17.
[12] 赵璇, 李新生, 邢丽红, 等. 高效液相色谱法测定汉中茶叶中茶氨酸含量[J]. 食品安全质量检测学报, 2015, 6(12): 4902-4907.
Zhao X, Li X S, Xing L H, et al.Determination of theanine in Hanzhong tea plantation by high performance liquid chromatography[J]. Journal of Food Safety and Quality, 2015, 6(12): 4902-4907.
[13] 中国标准出版社第一编辑室. 茶叶标准汇编[M]. 5版. 北京: 中国标准出版社, 2014.
The first editorial office of China Standards Press. Collection of tea standards [M]. 5th ed. Beijing: Standards Press of China, 2014.
[14] 高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
Gao J F.Experimental guidance of plant physiology [M]. Beijing: Higher Education Press, 2006.
[15] 薛金金, 江和源, 龙丹, 等. HPLC法同时测定茶叶中聚酯型儿茶素和茶黄素[J]. 中国食品学报, 2014, 14(5): 237-243.
Xue J J, Jiang H Y, Long D, et al.Simultaneous multiresidue determination of theasinensins and theaflavins in tea using high performance liquid chromatography[J]. Journal of Chinese Institute of Food Science and Technology, 2014, 14(5): 237-243.
[16] 田纪春, 王学臣, 刘广田. 植物的光合作用与光合氮、碳代谢的耦联及调节[J]. 生命科学, 2001, 13(4): 145-147.
Tian J C, Wang X C, Liu G T.The coupling and regulation between photosynthesis and nitrogen, carbon metabolism in plant[J]. Chinese Bulletin of Life Sciences, 2001, 13(4): 145-147.
[17] 张兰, 魏吉鹏, 沈晨, 等. 秋茶光合作用与品质成分变化的分析[J]. 茶叶科学, 2018, 38(3): 271-280.
Zhang L, Wei J P, Shen C, et al.Analysis of the photosynthesis and quality components changes in autumn tea[J]. Journal of Tea Science, 2018, 38(3): 271-280.
[18] 陈芳, 刘宇鹏, 谷晓平, 等. 低温对茶树光合特性及产量的影响[J]. 作物杂志, 2018(3): 155-161.
Chen F, Liu Y P, Gu X P, et al.Effects of low temperature on photosynthetic characteristics and yield of tea(Camellia sinensis L.)[J]. Crops, 2018(3): 155-161.
[19] 崔波, 程邵丽, 袁秀云, 等. 低温胁迫对白及光合作用及叶绿素荧光参数的影响[J]. 热带作物学报, 2019, 40(5): 891-897.
Cui B, Cheng S L, Yuan X Y, et al.Effects of low temperature stress on the photosynthetic characteristics and chlorophyll fluorescence parameters of Bletilla striata[J]. Chinese Journal of Tropical Crops, 2019, 40(5): 891-897.
[20] 周超凡, 吴春涛, 李丹丹, 等. 外源H₂S通过减轻低温光抑制增强黄瓜幼苗耐冷性[J]. 植物生理学报, 2018, 54(3): 411-420.
Zhou C F, Wu C T, Li D D, et al.Hydrogen sulfide promotes chilling tolerance of cucumber seedlings by alleviating low-temperature photoinhibition[J]. Plant Physiology Journal, 2018, 54(3): 411-420.
[21] 吴雪霞, 朱宗文, 张爱冬, 等. 外源褪黑素对低温胁迫下茄子幼苗生长及其光合作用和抗氧化系统的影响[J]. 西北植物学报, 2017, 37(12): 2427-2434.
Wu X X, Zhu Z W, Zhang A D, et al.Effects of exogenous melatonin on the growth, photosynthesis and antioxidant system in eggplant (Solanum melongena L.) seedlings under low temperature stress[J]. Acta Botanica Boreali Occidentalia Sinica, 2017, 37(12): 2427-2434.
[22] 余丽玲, 何天友, 陈凌艳, 等. 水杨酸对低温胁迫下西洋杜鹃光合作用和叶绿素荧光的影响[J]. 江西农业大学学报, 2014, 36(3): 520-525.
Yu L L, He T Y, Chen L Y, et al.Effects of salicylic acid on photosynthesis and chlorophyll fluorescence of rhododendron hybridum under low temperature stress[J]. Acta Agriculturae Universitatis Jiangxiensis, 2014, 36(3): 520-525.
[23] 李庆会, 徐辉, 周琳, 等. 低温胁迫对2个茶树品种叶片叶绿素荧光特性的影响[J]. 植物资源与环境学报, 2015, 24(2): 26-31.
Li Q H, Xu H, Zhou L, et al.Effect of low temperature stress on chlorophyll fluorescence characteristics in leaf of Camellia sinensis[J]. Journal of Plant Resources and Environment, 2015, 24(2): 26-31.
[24] 徐刚, 刘涛, 高文瑞, 等. ALA对低温胁迫下辣椒植株生长及光合特性的影响[J]. 江苏农业学报, 2011, 27(3): 612-616.
Xu G, Liu T, Gao W R, et al.Effects of 5-aminolevulinic acid (ALA) on growth and photosynthesis of pepper plants under low temperature stress[J]. Jiangsu Journal of Agricultural Sciences, 2011, 27(3): 612-616.
[25] 许大全. 光合作用气孔限制分析中的一些问题[J]. 植物生理学通讯, 1997(4): 241-244.
Xu D Q.Some Problems in stomatal limitation analysis of photosynthesis[J]. Plant Physiology Journal, 1997(4): 241-244.
[26] Wang L J, Jing W B, Huang B J.Promotion of 5-aminolevulinic acid on photosynthesis of melon (Cucumis melo) seedlings under low light and chilling stress conditions[J]. Physiologia Plantarum, 2004, 121(2): 258-264.
[27] 张治平, 汪良驹, 姚泉洪. 过量合成ALA转基因烟草叶片光合与叶绿素荧光特性的研究[J]. 西北植物学报, 2008(6): 1196-1202.
Zhang Z P, Wang L J, Yao Q H.Study on leaf photosynthesis and chlorophyll fluorescence of transgenic tobacco over-producing 5-aminolevulinic acid (ALA)[J]. Acta Botanica Boreali-Occidentalia Sinica, 2008(6): 1196-1202.
[28] 陈令会, 刘龙博, 安玉艳, 等. 外源5-氨基乙酰丙酸促进苹果叶片气孔开放机理的初探[J]. 园艺学报, 2014, 41(10): 1965-1974.
Chen L H, Liu L B, An Y Y, et al.Preliminary studies on the possible mechanism underlying 5-aminolevulinic acid-induced stomatal opening in apple leaves[J]. Acta Horticulturae Sinica, 2014, 41(10): 1965-1974.
[29] 安玉艳, 冯新新, 丁恒毅, 等. 5-氨基乙酰丙酸(5-ALA)对春茶生长与品质成分的影响[J]. 江苏农业科学, 2016, 44(11): 224-228.
An Y Y, Feng X X, Ding H Y, et al.Effects of 5-aminolevulinic acid on the growth and quality components of spring tea[J]. Jiangsu Agricultural Sciences, 2016, 44(11): 224-228.
[30] 康琅, 汪良驹. ALA对西瓜叶片叶绿素荧光光响应曲线的影响[J]. 南京农业大学学报, 2008, 31(1): 31-36.
Kang L, Wang L J.Effects of ALA treatments on light response curves of chlorophyll fluorescence of watermelon leaves[J]. Journal of Nanjing Agricultural University, 2008, 31(1): 31-36.
[31] 叶乃兴. 茶叶品质性状的构成与评价[J]. 中国茶叶, 2010, 32(8): 10-11.
Ye N X.Composition and evaluation of tea quality characters[J]. China Tea, 2010, 32(8): 10-11.
[32] 郭颖, 陈琦, 黄峻榕, 等. 茶叶滋味与其品质成分的关系[J]. 茶叶通讯, 2015, 42(3): 13-15, 28.
Guo Y, Chen Q, Huang J R.et al.The tea flavor quality and its ingredients[J]. Journal of Tea Communication, 2015, 42(3): 13-15, 28.
[33] 宛晓春. 茶叶生物化学[M]. 3版. 北京: 中国农业出版社, 2003: 217-219.
Wan X C.Tea biochemistry [M]. 3rd ed. Beijing: China Agriculture Press, 2003: 217-219.
[34] 王晓萍, 吴洵. 炒青绿茶的生化组分和微量元素综合评级[J]. 茶叶科学, 1991, 11(1): 45-49.
Wang X P, Wu X.Comprehensive evaluation of biochemical components and trace elements in roasted green tea[J]. Journal of Tea Science, 1991, 11(1): 45-49.
[35] 郭湘, 唐茜, 许燕, 等. 早春霜冻对不同茶树品种芽叶的生化成分及制茶品质的影响[J]. 云南大学学报(自然科学版), 2015, 37(6): 930-938.
Guo X, Tang Q, Xu Y, et al.Effect of frost in early spring on biochemical composition and sensory quality of new shoots in different tea cultivars[J]. Journal of Yunnan University (Natural Sciences Edition), 2015, 37(6): 930-938.
[36] 朱政, 蒋家月, 江昌俊, 等. 低温胁迫对茶树叶片SOD、可溶性蛋白和可溶性糖含量的影响[J]. 安徽农业大学学报, 2011, 38(1): 24-26.
Zhu Z, Jiang J Y, Jiang C J, et al.Effects of low temperature stress on SOD activity, soluble protein content and soluble sugar content in Camellia sinensis leaves[J]. Journal of Anhui Agricultural University, 2011, 38(1): 24-26.
[37] 卢克欢, 郭双, 韦悦, 等. 外源性5-氨基乙酰丙酸对盐胁迫下颠茄生理特性及次生代谢产物含量的影响[J]. 西南大学学报(自然科学版), 2018, 40(9): 13-20.
Lu K H, Guo S, Wei Y, et al.Effects of exogenous 5-aminolevulinic acid on physiological characteristics and secondary metabolite contents of Atropa belladonna L. seedlings under NaCl stress[J]. Journal of Southwest University (Natural Science Edition), 2018, 40(9): 13-20.
[38] 胡云鹏, 康念, 李宇辉, 等. 5-氨基乙酰丙酸对百合生长发育的影响[J]. 仲恺农业工程学院学报, 2017, 30(2): 1-5.
Hu Y P, Kang N, Li Y H, et al.Effects of 5-aminolevulinic acid on growth and development of lily[J]. Journal of Zhongkai University of Agriculture and Engineering, 2017, 30(2): 1-5.
[39] 龙志荣, 王登良, 邱瑞瑾, 等. 水解酶对乌龙茶品质形成的影响[J]. 广东茶业, 2007(1): 10-14.
Long Z R, Wang D L, Qiu R J, et al.Effect of hydrolase on quality formation of oolong tea[J]. Guangdong Tea Industry, 2007(1): 10-14
[40] 曹慧明, 史作民, 周晓波, 等. 植物对低温环境的响应及其抗寒性研究综述[J]. 中国农业气象, 2010, 31(2): 310-314, 319.
Cao H M, Shi Z M, Zhou X B, et al. A review on response of plant to low temperature and its cold resistance [J]. Chinese Journal of Agrometeorology, 2010, 31(2): 310-314, 319.
[41] Ashworth E N, Pearce R S.Extracellular freezing in leaves of freezing-sensitive species[J]. Planta, 2002, 214(5): 798-805.