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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (6): 723-730.doi: 10.13305/j.cnki.jts.2019.06.011

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The Role of Plasma Membrane H+-ATPase on Nitrogen-regulated Phosphorus Uptake in Tea Plants

CHEN Linmu1, CHEN Jingguang2, WANG Ningning1, ZHANG Xianchen1,*   

  1. 1. State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China;
    2. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518116, China
  • Received:2018-12-30 Revised:2019-03-13 Online:2019-12-15 Published:2019-12-24

Abstract: Phosphorus is one of the most important mineral elements for plant growth and development, and different nitrogen forms can regulate phosphorus uptake. As is well-known that plant plasma membrane (PM) H+-ATPase plays an important role in nutrition uptake, it may also relate to the different nitrogen-modulated phosphorus uptake. In our study, PM H+-ATPase in tea roots were isolated by two-phase partitioning in aqueous dextranT-500 and polyethylene glycol, and the effect of different nitrogen forms on phosphorus uptake and the characteristic parameters of PM H+-ATPase in tea roots were examined by using non-invasive micro-test technique and Western-blot. It was found that ammonium significantly improved the phosphorus accumulation in tea plants as compared with nitrate nutrition. In addition, less depolarized PM potentials, higher net H+ flux, PM H+-ATPase activities and protein levels were found under ammonium treatment than those under nitrate nutrition treatment. Furthermore, vanadate (the PM H+-ATPase inhibitor) significantly decreased phosphorus accumulation in tea plants under ammonium and nitrate nutrition treatments, which further suggests that PM H+-ATPase may be involved in phosphorus accumulation in tea plants regulated by different nitrogen forms.

Key words: ammoniacal nitrogen, nitrate nitrogen, phosphorus absorption, PM H+-ATPase, tea plants

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