Effects of Nitrogen Form and Weak Light Stress on Tea Plant Growth and Metabolism

SHEN Ruihan; MA Lifeng; YANG Xiangde; FANG Li

doi:10.13305/j.cnki.jts.2023.03.01

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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (3) : 349-355. DOI: 10.13305/j.cnki.jts.2023.03.01

Research Paper

Effects of Nitrogen Form and Weak Light Stress on Tea Plant Growth and Metabolism

SHEN Ruihan^1,2, MA Lifeng^1,*, YANG Xiangde¹, FANG Li¹

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Abstract

In order to analyze the effects of N form and weak light stress on the tea plant growth and metabolism, the two-year-old cutting seedlings of ‘Longjing 43’ were taken as experimental materials, and two different forms of N sources (NH₄⁺-N and NO₃^--N) and two light intensities (normal light and weak light) were supplied under solution culture. The results show that: (1) the net photosynthetic rate of tea seedlings was mainly affected by the N form under normal light intensity. The net photosynthetic rate of tea seedlings treated with NH₄⁺-N was significantly higher than that treated with NO₃^--N. Under the same nitrogen conditions, compared to normal light, weak light stress had a tendency to reduce the net photosynthetic rate of tea seedlings. (2) Under normal light intensity, the chlorophyll content in young shoots treated with NH₄⁺-N was significantly higher than that treated with NO₃^--N, but the chlorophyll a (Chl a)/chlorophyll b (Chl b) ratio was basically unchanged. The chlorophyll content increased significantly after weak light stress, but Chl a/b ratio under NH₄⁺-N treatment was significantly increased, while Chl a/b ratio under NO₃^--N treatment was slightly decreased. NO₃^--N treated tea plants showed better shade tolerance than that treated with NH₄⁺-N. (3) N uptake by tea seedlings was mainly influenced by both N form and light, and weak light stress helped to improve the accumulation of N in new shoots of tea seedlings. The N content of NH₄⁺-N treated tea seedlings was significantly higher than that treated with NO₃^--N under normal light. Weak light stress significantly increased the above-ground N content in tea plants. (4) Under normal light intensity, the contents of free amino acids and caffeine in young shoots treated with NH₄⁺-N were significantly higher than those treated with NO₃^--N, while the ratio of tea polyphenol to free amino acids was lower. After weak light stress, the contents of free amino acid and caffeine in shoots further increased, while the contents of tea polyphenol and the ratio of tea polyphenol to free amino acids continued to decline. The increase rate of total free amino acid and descent rate of tea polyphenol and tea polyphenol/free amino acids ratio were faster in young shoots treated with NO₃^--N than those treated with NH₄⁺-N. The composition of shoot quality was mainly affected by light intensity, but tea polyphenol-free amino acids ratio was influenced by both light intensity and N form. The research results provide theoretical basis for the analysis of its mechanism.

Key words

ammonium nitrogen / light intensity / nitrate nitrogen / photosynthesis / quality component / tea plant

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SHEN Ruihan, MA Lifeng, YANG Xiangde, FANG Li. Effects of Nitrogen Form and Weak Light Stress on Tea Plant Growth and Metabolism[J]. Journal of Tea Science. 2023, 43(3): 349-355 https://doi.org/10.13305/j.cnki.jts.2023.03.01

References

[1] Le G G, Colquhoun I, Defernez M.Metabolite profiling using ¹H NMR spectroscopy for quality assessment of green tea (Camellia sinensis L.)[J]. Agricultural and Food Chemistry, 2004, 52(4): 692-700.
[2] Lee J E, Lee B J, Chung J O, et al.Metabolomic unveiling of a diverse range of green tea (Camellia sinensis) metabolites dependent on geography[J]. Food Chemistry, 2015, 174: 452-459.
[3] Lee J E, Lee B J, Hwang J A, et al.Metabolic dependence of green tea on plucking positions revisited: a metabolomic study[J]. Agricultural and Food Chemistry, 2011, 59(19): 10579-10585.
[4] Bloom A J.The increasing importance of distinguishing among plant nitrogen sources[J]. Current Opinion in Plant Biology, 2015, 25: 10-16.
[5] Huang H, Yao Q Y, Xia E H, et al.Metabolomics and transcriptomics analyses reveal nitrogen influences on the accumulation of flavonoids and amino acids in young shoots of tea plant (Camellia sinensis L.) associated with tea flavor[J]. Journal of Agricultural and Food Chemistry, 2018, 66(37): 9828-9838.
[6] Ruan J Y, Gerendas J, Hardter R, et al.Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis L.) plants[J]. Annals of Botany, 2007, 99(2): 301-310.
[7] Liu M Y, Burgos A, Zhang Q F, et al.Analyses of transcriptome profiles and selected metabolites unravel the metabolic response to NH₄⁺ and NO₃^- as signaling molecules in tea plant (Camellia sinensis L.)[J]. Scientia Horticulturae, 2017, 218: 293-303.
[8] Zhang Y, Xia G H, Ma K, et al.Effects of shade on photosynthetic characteristics and chlorophyll fluorescence of Ardisia violacea[J]. Chinese Journal of Applied Ecology, 2014, 25(7): 1940-1948.
[9] Ji H G, Lee Y R, Lee M S, et al.Diverse metabolite variations in tea (Camellia sinensis L.) leaves grown under various shade conditions revisited: a metabolomics study[J]. Journal of Agricultural and Food Chemistry, 2018, 66(8): 1889-1897.
[10] Sano T, Horie H, Matsunaga A, et al.Effect of shading intensity on morphological and color traits and on chemical components of new tea (Camellia sinensis L.) shoots under direct covering cultivation[J]. Journal of the Science of Food and Agriculture, 2018, 98(15): 5666-5676.
[11] 骆科胜, 李峪, 兰方强, 等. 贵州碾茶茶园覆网方式浅析[J]. 中国茶叶, 2021, 43(2): 60-64.
Luo S K, Li Y, Lan F Q, et al.Analysis on sunshade net covering modes in tea garden in Guizhou[J]. China Tea, 2021, 43(2): 60-64.
[12] Yang T Y, Xie Y X, Lu X, et al.Shading promoted theanine biosynthesis in the roots and allocation in the shoots of the tea plant (Camellia sinensis L.) cultivar Shuchazao[J]. Journal of Agricultural and Food Chemistry, 2021, 69(16): 4795-4803.
[13] Hikosaka K, Terashima I.A model of the acclimation of photosynthesis in the leaves of C₃ plants to sun and shade with respect to nitrogen use[J]. Plant Cell and Environment, 1995, 18(6): 605-618.
[14] Ku K M, Choi J N, Kim J, et al.Metabolomics analysis reveals the compositional differences of shade grown tea (Camellia sinensis L.)[J]. Journal of Agricultural and Food Chemistry, 2010, 58(1): 418-426.
[15] Ruan J Y, Gerendás J, Härdter R, et al.Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis L.) plants[J]. Annals of Botany, 2007, 99(2): 301-310.
[16] Wellburn A R, Lichtenthaler H K.Formulae and program to determine total carotenoids and chlorophylls a and b of leaf extracts in different solvents[C]//Sybesma C. Advances in photosynthesis research springer netherlands. Dordrecht: Springer, 1984.
[17] 杨伟红, 郭晋平, 贾鹏飞. 遮阴对盆栽茶梅叶片内营养物质的影响[J]. 山西农业科学, 2009, 37(12): 18-19, 24.
Yang W H, Guo J P, Jia P F.Effects of shading treatments to leave nutrients of the potted Gamellia Sasangua in greenhouse[J]. Journal of Shanxi Agricultural Sciences, 2009, 37(12): 18-19, 24.
[18] Song R, Kelman D, Johns K L, et al.Correlation between leaf age, shade levels, and characteristic beneficial natural constituents of tea (Camellia sinensis L.) grown in Hawaii[J]. Food Chemistry, 2012, 133(3): 707-714.
[19] 李芯妍, 滕志远, 徐启江, 等. 遮荫对黑茶藨子叶片形态结构和光合特性的影响[J]. 植物研究, 2017, 37(4): 521-528.
Li X Y, Teng Z Y, Xu Q J, et al.Effects of shading on anatomical structure and photosynthetic characteristics of Ribes nigrum L. leaves[J]. Bulletin of Botanical Research, 2017, 37(4): 521-528.
[20] 闫小莉, 王德炉. 遮荫对苦丁茶树叶片特征及光合特性的影响[J]. 生态学报, 2014, 34(13): 3538-3547.
Yan X L,Wang D L.Effects of shading on the leaves and photosynthetic characteristics of Ligustrum robustum[J]. Acta Ecologica Sinica, 2014, 34(13): 3538-3547.
[21] Okano K, Chutani K, Matsuo K.Suitable level of nitrogen fertilizer for tea (Camellia sinensis L.) plants in relation to growth, photosynthesis, nitrogen uptake and accumulation of free amino acids[J]. Japanese Journal of Crop Science, 1997, 66(2): 279-287.
[22] Yang T Y, Li H P, Tai Y L, et al.Transcriptional regulation of amino acid metabolism in response to nitrogen deficiency and nitrogen forms in tea plant root (Camellia sinensis L.)[J]. Scientific Reports, 2020, 10(1): 6868. doi: 10.1038/s41598-020-63835-6.