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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (6): 768-778.doi: 10.13305/j.cnki.jts.2022.06.012

• Research Paper • Previous Articles     Next Articles

Preliminary Study on SNP Molecular Markers in Tea Chloroplast Genome

LUO Xiangzong1,2, HU Yunfei1, WU Linhui1, ZHAO Yaqi1, ZHENG Weiming1, LI Xiangru3, LI Li1,*   

  1. 1. Wuyi University College of Tea and Food, Nanping 354300, China;
    2. Anhui Agricultural University College of Tea and Food Technology, Hefei 230036, China;
    3. Fujian Agriculture and Forestry University, College of Horticulture, Fuzhou 350002, China
  • Received:2022-09-09 Revised:2022-11-01 Online:2022-12-15 Published:2023-01-04

Abstract: The application value of traditional chloroplast gene molecular markers in tea section classification is limited relatively. The aim of this study was to screen out SNP (single nucleotide polymorphism) combinations that could be used for tea plant identification and maternal traceability. In this study, the chloroplast genome sequences of 18 reported tea plants were compared. By designing universal primers, the candidate molecular markers were amplified and analyzed by sanger sequencing in 169 tea cultivars/strains, and 16 pairs of primers containing 25 SNP loci were screened out. This set of SNP molecular markers can be used for maternal traceability and identification analysis of tea cultivars. In addition, the DNA fingerprints composed of SNP sites were digitally encoded in combination with the basic information of tea resources, and finally a 30-digit molecular ID card of tea resources was formed, and corresponding barcodes and two-dimensional codes were constructed for cultivar identification. The data from this study provided a new idea for maternal origin tracing and identification of tea cultivars.

Key words: chloroplast gene, SNP molecular markers, maternal traceability, identification, ID card

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