A Primary Study on Sex Pheromone of Ectropis grisescens

LUO Zongxiu; LI Zhaoqun; CAI Xiaoming; BIAN Lei; CHEN Zongmao

doi:10.13305/j.cnki.jts.2016.05.013

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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (5) : 537-543. DOI: 10.13305/j.cnki.jts.2016.05.013

A Primary Study on Sex Pheromone of Ectropis grisescens

LUO Zongxiu^1,2, LI Zhaoqun¹, CAI Xiaoming¹, BIAN Lei¹, CHEN Zongmao^1,*

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Abstract

Ectropis grisescens Warren is a severe defoliator species in tea garden of China. In this study, the sex pheromone was extracted from abdominal tips of virgin female moths by n-hexane during peak calling period. Analysis of gland extracts by gas chromatography (GC) with electroantennographic detection (EAD) and the components of sex pheromone were identified by gas chromatography (GC) coupled to mass spectrometry (MS). Finally, the biological activity of synthetic sex pheromone was evaluated by field trapping. The result of GC-EAD revealed two components that elicited responses from male moth antennae. Based on the mass spectrum, two components were identified as (Z,Z,Z)-3,6,9-octadecatriene and (Z,Z)-3,9-cis-6,7-epoxy-octadecadiene. In field experiment, different attracting effects were identified under different ratio of sex pheromones. Experiment showed the ratio of 4:6 of these synthetic components showed the maximum attractiveness at 1.0 mg when loaded on rubber septum. This investigation provides a theoretical basis for developing sex pheromone application technology againest Ectropis grisescens.

Key words

Ectropis grisescens / identification / sex pheromone

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LUO Zongxiu, LI Zhaoqun, CAI Xiaoming, BIAN Lei, CHEN Zongmao. A Primary Study on Sex Pheromone of Ectropis grisescens[J]. Journal of Tea Science. 2016, 36(5): 537-543 https://doi.org/10.13305/j.cnki.jts.2016.05.013

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