两个EoNPV毒株对茶尺蠖和灰茶尺蠖的毒力差异

doi:10.13305/j.cnki.jts.2021.04.008

Abstract

Abstract: The virulence differences of Ectropis obliqua nucleopolyhedrovirus (EoNPV) from different isolates to Ectropis obliqua and Ectropis grisescens were analyzed, which provided a basis for effectively improving the control effect of EoNPV. The virulence of EoNPV Zhejiang strain (EoNPV-ZJ) and EoNPV Jiangxi strain (EoNPV-JX) to third instar larvae of E. obliqua and E. grisescens were determined by leaf dipping. The homologous regions (hrs) of EoNPV-ZJ and EoNPV-JX were further sequenced and analyzed by multiple sequence alignment. The results indicate that the 14 d LC₅₀ of EoNPV-JX to E. grisescens and E. obliqua were 5.95×10⁶ PIB·mL^-1 and 3.14×10⁶ PIB·mL^-1, respectively, while the 14 d LC₅₀ of EoNPV-ZJ to the two tea geometrid moths were 1.13×10⁷ PIB·mL^-1 and 5.04×10⁶ PIB·mL^-1, respectively. The hr1 of EoNPV-JX and EoNPV-ZJ were both 1 795 bp in size, containing 11 complete palindrome sequences, and hr3 were both 665 bp in size, containing 3 complete palindrome sequences, which were the same as EoNPV Anhui strain (EoNPV-AH). However, the hr2 regions of the three strains were different. The hr2 of EoNPV-JX was 864 bp in size and contained 7 complete palindrome sequences, and it was 1 168 bp in size and contained 12 complete palindrome sequences in EoNPV-ZJ, all of which were less than the 18 complete palindrome sequences of hr2 in EoNPV-AH. Our study suggests that the virulence of different strains of EoNPV to E. obliqua were higher than that of its related species, E. grisescens, while the toxicity of EoNPV-JX to E. grisescens was more than that of EoNPV-ZJ. The sensitivity of E. obliqua to EoNPV was higher than that of E. grisescens. The virulence of EoNPV might be related to the number of palindromes of hr2.

Key words: EoNPV, strain, Ectropis obliqua, Ectropis grisescens, virulence, homologous regions

CLC Number:

XU Bin, HAN Guangjie, QI Jianhang, LI Chuanming, XU Jian, LU Yurong, LIU Qin. Virulence Difference of Two Strains of EoNPV Isolates to Ectropis obliqua and Ectropis grisescens[J]. Journal of Tea Science, 2021, 41(4): 545-552.

References

[1] 姜楠, 刘淑仙, 薛大勇, 等. 我国华东地区两种茶尺蛾的形态和分子鉴定[J]. 应用昆虫学报, 2014, 51(4): 987-1002.
Jiang N, Liu S X, Xue D Y, et al.External morphology and molecular identification of two tea geometrid moth from southern China[J]. Chinese Journal of Applied Entomology, 2014, 51(4): 987-1002.
[2] 葛超美, 殷坤山, 唐美君, 等. 灰茶尺蠖的生物学特性[J]. 浙江农业学报, 2016, 28(3): 464-468.
Ge C M, Yin K S, Tang M J, et al.Biological characteristics of Ectropis grisescens Warren[J]. Acta Agriculturae Zhejiangensis, 2016, 28(3): 464-468.
[3] 张昌娣. 茶树茶毛虫与茶尺蠖的发生与防治[J]. 现代农村科技, 2013(10): 29.
Zhang C D.Occurrence and control of tea caterpillar and tea looper[J]. Modern Rural Technology, 2013(10): 29.
[4] 李喜旺, 刘丰静, 邵胜荣, 等. 茶尺蠖绿色防控技术研究现状及展望[J]. 茶叶科学, 2017, 37(4): 325-331.
Li X W, Liu F J, Shao S R, et al.Research progress and prospect of green control techniques of Ectropis obliqua[J]. Journal of Tea Science, 2017, 37(4): 325-331.
[5] 郭慧芳, 方继朝, 韩召军. 昆虫病毒增效剂研究进展[J]. 昆虫学报, 2003, 46(6): 766-772.
Guo H F, Fang J C, Han Z J.Advances in insect virus synergists[J]. Acta Entomologica Sinica, 2003, 46(6): 766-772.
[6] 席羽. 茶尺蠖地理种群对茶尺蠖核型多角体病毒的敏感性差异及遗传变异研究[D]. 北京: 中国农业科学院, 2011.
Xi Y.Susceptibility variation against Ectropis obliqua nucleopolyhedrovirus and variation in geographic populations of tea geometrid, Ectropis obliqua prout [D]. Beijing: Chinese Academy of Agricultural Sciences, 2011.
[7] 王晓庆, 彭萍, 胡翔, 等. 茶尺蠖对不同品系EoNPV敏感性的地理差异研究[J]. 西南农业学报, 2013, 26(4): 1062-1065.
Wang X Q, Peng P, Hu X, et al.Geographical difference of susceptibility of Ectropis obliqua (Prout) to different EoNPV strains[J]. Southwest China Journal of Agricultural Sciences, 2013, 26(4): 1062-1065.
[8] 王晓庆, 冉烈, 彭萍, 等. EoNPV不同品系的致病力及增效作用的研究[J]. 西南大学学报(自然科学版), 2015, 37(3): 48-52.
Wang X Q, Ran L, Peng P, et al.Study on the virulence and the synergistic effect of EoNPV isolates against the larvae of geometrid Ectropis obliqua Prout[J]. Journal of Southwest China Normal University (Natural Science Edition), 2015, 37(3): 48-52.
[9] 刘琴, 徐健, 李传明, 等. 人工饲料条件下茶尺蠖的饲养与繁殖[J]. 茶叶科学, 2015, 35(4): 323-328.
Liu Q, Xu J, Li C M, et al.Growth and reproduction of Ectropis obliqua fed on artificial diet[J]. Journal of Tea Science, 2015, 35(4): 323-328.
[10] 唐美君. 茶尺蠖病毒制剂生产与应用技术的研究及推广[D]. 杭州: 浙江大学, 2008.
Tang M J.Studies on the production and application of EoNPV preparations for the management of the tea looper, Ectropis obliqua [D]. Hangzhou: Zhejiang University, 2008.
[11] 刘丽华, 王利群, 李兵, 等. 柞蚕多角体病毒的纯化和鉴定[J]. 应用与环境生物学报, 2005, 11(1): 112-114.
Liu L H, Wang L Q, Li B, et al.Purification and identification of Antheraea Pernyi nuclear polyhedrosis virus[J]. Chinese Journal of Applied and Environmental Biology, 2005, 11(1): 112-114.
[12] 鲁艳辉, 田俊策, 郑许松, 等. 室内测定6种化学杀虫剂对草地贪夜蛾幼虫的毒力[J]. 环境昆虫学报, 2020, 42(2): 329-334.
Lu Y H, Tian J C, Zheng X S, et al.Laboratory toxicity test of 6 chemical insecticides against Spodoptera frugiperda[J]. Journal of Environmental Entomology, 2020, 42(2): 329-334.
[13] 张益民, 赵怀宇, 张建红, 等. 茶尺蠖核型多角体病毒研究[J]. 生物防治通报, 1989, 5(4): 168-172.
Zhang Y M, Zhao H Y, Zhang J H, et al.Study on the Ectropis obliqua nucleopolyhedrovirus[J]. Chinese Journal of Biological Control, 1989, 5(4): 168-172.
[14] 殷坤山, 陈华才, 肖强, 等. 茶尺蠖核型多角体病毒制剂的试制与推广应用[J]. 中国病毒学, 2000, 15(1): 81-84.
Yin K S, Chen H C, Xiao Q, et al.The development, promotion and application of the Ectropis obliqua nucleopolyhedrovirus preparations[J]. Virologica Sinica, 2000, 15(1): 81-84.
[15] 席羽, 殷坤山, 肖强. 不同地理种群茶尺蠖对EoNPV的敏感性差异研究[J]. 茶叶科学, 2011, 31(2): 100-104.
Xi Y, Yin K S, Xiao Q.The susceptibility difference against EoNPV in different geographic populations of tea geometrid (Ectropic oblique prout)[J]. Journal of Tea Science, 2011, 31(2): 100-104.
[16] 席羽, 殷坤山, 唐美君, 等. 浙江茶尺蠖地理种群已分化成为不同种[J]. 昆虫学报, 2014, 57(9): 1117-1122.
Xi Y, Yin K S, Tang M J, et al.Geographic populations of the tea geometrid, Ectropis obliqua (Lepidoptera: Geometridae) in Zhejiang, eastern China have differentiated into different species[J]. Acta Entomologica Sinica, 2014, 57(9): 1117-1122.
[17] 唐美君, 郭华伟, 葛超美, 等. EoNPV对灰茶尺蠖的致病特性及高效毒株筛选[J]. 浙江农业学报, 2017, 29(10): 1686-1691.
Tang M J, Guo H W, Ge C M, et al.Pathogenic characters of Ectropis obliqua nucleopolyhedroviruses on Ectropis grisescens Warren and screening of high efficient strain[J]. Acta Agriculturae Zhejiangensis, 2017, 29(10): 1686-1691.
[18] 张海波, 王风良, 陈永明, 等. 核型多角体病毒对玉米草地贪夜蛾的控制作用研究[J]. 植物保护, 2020, 46(2): 254-260.
Zhang H B, Wang F L, Chen Y M, et al.Study on the control effect of Nucleopolyhedrovirus on Spodoptera frugiperda[J]. Plant Protection, 2020, 46(2): 254-260.
[19] 张秀霞, 白婷婷, 毛晓红, 等. 4种微生物杀虫剂对甜菜夜蛾的室内毒力及田间防效评价[J]. 山东农业科学, 2020, 52(4): 141-145.
Zhang X X, Bai T T, Mao X H, et al.Toxicity test in laboratory and control efficacy in field of four microbial insecticides to Spodoptera exigua[J]. Shandong Agricultural Sciences, 2020, 52(4): 141-145.
[20] 白家赫, 王志博, 肖强. 浙江茶区茶尺蠖两近缘种的遗传分化及分布[J]. 昆虫学报, 2018, 61(6): 741-748.
Bai J H, Wang Z B, Xiao Q.Genetic differentiation and distribution of two sibling species of tea geometrids in tea-growing areas in Zhejiang, eastern China[J]. Acta Entomologica Sinica, 2018, 61(6): 741-748.
[21] Wang Z B, Bai J H, Liu Y J, et al.Transcriptomic analysis reveals insect hormone biosynthesis pathway involved in desynchronized development phenomenon in hybridized sibling species of tea geometrids (Ectropis grisescens and Ectropis obliqua)[J]. Insects, 2019, 10(11): 381. doi: 10.3390/insects10110381.
[22] Cory J S, Green B M, Paul R K, et al.Genotypic and phenotypic diversity of a baculovirus population within an individual insect host[J]. Journal of Invertebrate Pathology, 2005, 89(2): 101-111.
[23] Larem A, Ben-Tiba S, Wennmann J T, et al.Elucidating the genetic diversity of Phthorimaea operculella granulovirus (PhopGV)[J]. Journal of General Virology, 2019, 100(4): 679-690.
[24] Wennmann J T, Radtke P, Eberle K E, et al.Deciphering single nucleotide polymorphisms and evolutionary trends in isolates of the Cydia pomonella granulovirus[J]. Viruses, 2017, 9(8): 227. doi: 10.3390/v9080227.
[25] Loiseau V, Herniou E A, Moreau Y, et al.Wide spectrum and high frequency of genomic structural variation, including transposable elements, in large double-stranded DNA viruses[J]. Virus Evolution, 2020, 6(1): 60. doi: 10.1093/ve/vez060.
[26] Martin D W, Weber P C.DNA replication promotes high-frequency homologous recombination during Autographa californica multiple nuclear polyhedrosis virus infection[J]. Virology, 1997, 232(2): 300-309.
[27] Zwart M P, Ali G, Strien E A V, et al. Identification of loci associated with enhanced virulence in Spodoptera litura nucleopolyhedrovirus isolates using deep sequencing[J]. Viruses, 2019, 11(9): 872. doi: 10.3390/v11090872.
[28] Ali G, Abma-Henkens M H C, Werf W V D, et al. Genotype assembly, biological activity and adaptation of spatially separated isolates of Spodoptera litura nucleopolyhedrovirus[J]. Journal of Invertebrate Pathology, 2018, 153: 20-29.
[29] Maike B, Carstens E B.Sequential deletion of AcMNPV homologous regions leads to reductions in budded virus production and late protein expression[J]. Virus Research, 2018, 256: 125-133.
[30] Pearson M, Bjornson R, Pearson G, et al.The Autographa californica baculovirus genome: evidence for multiple replication origins[J]. Science, 1992, 257(5075): 1382-1384.
[31] Lin X, Chen Y, Yi Y, et al.Baculovirus immediately early 1, a mediator for homologous regions enhancer function in trans[J]. Virology Journal, 2010, 7(1): 32. doi: 10.1186/1743-422X-7-32.
[32] Carstens E B, Wu Y.No single homologous repeat region is essential for DNA replication of the baculovirus Autographa californica multiple nucleopolyhedrovirus[J]. Journal of General Virology, 2007, 88(1): 114-122.
[33] Nagai S, Alves C A F, Kobayashi M, et al. Comparative transient expression assay analysis of hycu-hr6- and IE1-dependent regulation of baculovirus gp64 early promoters in three insect cell lines[J]. Virus Research, 2011, 155(1): 83-90.

[1]	. [J]. Journal of Tea Science, 1965, 2(04): 59 -63 .
[2]	. [J]. Journal of Tea Science, 1965, 2(03): 18 -21 .
[3]	. [J]. Journal of Tea Science, 1965, 2(03): 22 -24 .
[4]	. [J]. Journal of Tea Science, 1966, 3(02): 55 -57 .
[5]	. [J]. Journal of Tea Science, 1966, 3(02): 82 -83 .
[6]	. [J]. Journal of Tea Science, 1986, 6(01): 1 -8 .
[7]	. [J]. Journal of Tea Science, 1987, 7(02): 29 -34 .
[8]	. [J]. Journal of Tea Science, 1988, 8(01): 37 -42 .
[9]	. [J]. Journal of Tea Science, 1995, 15(02): 154 .
[10]	. [J]. Journal of Tea Science, 1999, 19(01): 73 -76 .

Virulence Difference of Two Strains of EoNPV Isolates to Ectropis obliqua and Ectropis grisescens

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 10

[1]	YAN Yuting, LI Yujie, WANG Qian, TANG Meijun, GUO Huawei, LI Hongliang, SUN Liang. The Expression Profiles of Chemosensory Protein 8 Orthologs in Two Closely Related Tea Geometrid Species, Ectropis obliqua Prout and Ectropis grisescens Warren [J]. Journal of Tea Science, 2022, 42(2): 200-210.
[2]	YAN Yuting, WU Fan, ZHANG Yali, FU Xiaobin, CUI Hongchun, HAN Baoyu, LI Hongliang. Study on the Differences in Ligand-binding Function and Mode of the Antennal High-abundance Odorant-binding Proteins EoblOBP9 and EoblOBP11 of the Tea Geometrid, Ectropis obliqua Prout [J]. Journal of Tea Science, 2021, 41(5): 643-653.
[3]	WANG Zhibo, BAI Jiahe, ZHOU Xiaogui, GUO Huawei, XIAO Qiang. Effect of Three Antibiotic Treatments on Bacterial Endosymbiont Community of Ectropis Grisescens Warren [J]. Journal of Tea Science, 2021, 41(1): 90-100.
[4]	WANG Dingfeng, LI Liangde, LI Huiling, ZHANG Hui, WU Guangyuan. Screening, Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus [J]. Journal of Tea Science, 2021, 41(1): 101-112.
[5]	QIAO Li, HONG Feng, JIN Yinli, GENG Shubao, GUO Shibao. Effects on the Photoreaction Behavior of Ectropis Grisescens Warren to Different Light Spectrum [J]. Journal of Tea Science, 2020, 40(5): 617-624.
[6]	LIU Fengjing, RAN Wei, LI Xiwang, WANG Suqin, SUN Xiaoling. The Comparison of Leaf Structures and Empoasca Onukii Matsuda Honeydew Excretion among Five Tea Cultivars (Strains) [J]. Journal of Tea Science, 2020, 40(5): 625-631.
[7]	ZHANG Xin, Chen Chengcong, DU Qin, LI Xiwang, SUN Xiaoling. Cloning and Expression Analysis of Serine Protease EoSP1 in Tea Geometrid (Ectropis obliqua) and Its Response to Starvation [J]. Journal of Tea Science, 2019, 39(6): 669-680.
[8]	ZHANG Jiaxia, SUN Qinyu, GE Chaomei, YE Tao, ZHANG Ran, DING Yong. Morphological Characteristics and Histological Structure of the Reproductive Organs in Female Adults of Ectropis grisescens Warren (Lepidoptera: Geometridae) [J]. Journal of Tea Science, 2019, 39(1): 98-104.
[9]	WANG Dingfeng, LI Liangde, LI Huiling, ZHANG Hui, WANG Qingsen, WU Guangyuan. Molecular Identification and Virulence Evaluation of an Entomopathogenic Fungus Isolated from the Cadaver of Sympiezomias citri [J]. Journal of Tea Science, 2018, 38(3): 313-318.
[10]	RAN Wei, ZHANG Jin, ZHANG Xin, LIN Songbo, SUN Xiaoling. Infestation of Ectropis obliqua Affects the Catechin Metabolism in Tea Plants [J]. Journal of Tea Science, 2018, 38(2): 133-139.
[11]	LUO Zongxiu, SU Liang, LI Zhaoqun, LIU Yan, CAI Xiaoming, BIAN Lei, XIN Zhaojun, CHEN Zongmao. Field Application Technology of Sex Pheromone on Ectropis grisescens [J]. Journal of Tea Science, 2018, 38(2): 140-145.
[12]	LI Xiwang, LIU Fengjing, SHAO Shengrong, SU Liang, JIN Limeng, LOU Yonggen, SUN Xiaoling. Research Progress and Prospect of Green Control Techniques of Ectropis obliqua [J]. Journal of Tea Science, 2017, 37(4): 325-331.
[13]	WANG Dingfeng, LI Liangde, LI Jianlong, LI Huiling, ZHANG Hui, WANG Qingsen, WU Guangyuan. Screening of the Beauveria bassiana Strain with High Virulence to Basilepta melanopus (Coleoptera: Chrysomeloidea) [J]. Journal of Tea Science, 2017, 37(3): 229-236.
[14]	CHEN Changsong, WANG Feng, CHEN Yuzhen, SHAN Ruiyang, WANG Xiuping, LIN Zhenghe, ZHONG Qiusheng, CHEN Zhihui, YU Wenquan. Comparison of Leaf Functional and Photosynthetic Characteristics in Baijiguan Half-sib Teas [J]. Journal of Tea Science, 2016, 36(5): 452-460.
[15]	LUO Zongxiu, LI Zhaoqun, CAI Xiaoming, BIAN Lei, CHEN Zongmao. A Primary Study on Sex Pheromone of Ectropis grisescens [J]. Journal of Tea Science, 2016, 36(5): 537-543.