Virulence Difference of Two Strains of EoNPV Isolates to Ectropis obliqua and Ectropis grisescens

XU Bin; HAN Guangjie; QI Jianhang; LI Chuanming; XU Jian; LU Yurong; LIU Qin

doi:10.13305/j.cnki.jts.2021.04.008

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (4) : 545-552. DOI: 10.13305/j.cnki.jts.2021.04.008

Research Paper

Virulence Difference of Two Strains of EoNPV Isolates to Ectropis obliqua and Ectropis grisescens

XU Bin¹, HAN Guangjie¹, QI Jianhang², LI Chuanming¹, XU Jian¹, LU Yurong¹, LIU Qin^1,*

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Abstract

The virulence differences of Ectropis obliqua nucleopolyhedrovirus (EoNPV) from different isolates to Ectropis obliqua and Ectropis grisescens were analyzed, which provided a basis for effectively improving the control effect of EoNPV. The virulence of EoNPV Zhejiang strain (EoNPV-ZJ) and EoNPV Jiangxi strain (EoNPV-JX) to third instar larvae of E. obliqua and E. grisescens were determined by leaf dipping. The homologous regions (hrs) of EoNPV-ZJ and EoNPV-JX were further sequenced and analyzed by multiple sequence alignment. The results indicate that the 14 d LC₅₀ of EoNPV-JX to E. grisescens and E. obliqua were 5.95×10⁶ PIB·mL^-1 and 3.14×10⁶ PIB·mL^-1, respectively, while the 14 d LC₅₀ of EoNPV-ZJ to the two tea geometrid moths were 1.13×10⁷ PIB·mL^-1 and 5.04×10⁶ PIB·mL^-1, respectively. The hr1 of EoNPV-JX and EoNPV-ZJ were both 1 795 bp in size, containing 11 complete palindrome sequences, and hr3 were both 665 bp in size, containing 3 complete palindrome sequences, which were the same as EoNPV Anhui strain (EoNPV-AH). However, the hr2 regions of the three strains were different. The hr2 of EoNPV-JX was 864 bp in size and contained 7 complete palindrome sequences, and it was 1 168 bp in size and contained 12 complete palindrome sequences in EoNPV-ZJ, all of which were less than the 18 complete palindrome sequences of hr2 in EoNPV-AH. Our study suggests that the virulence of different strains of EoNPV to E. obliqua were higher than that of its related species, E. grisescens, while the toxicity of EoNPV-JX to E. grisescens was more than that of EoNPV-ZJ. The sensitivity of E. obliqua to EoNPV was higher than that of E. grisescens. The virulence of EoNPV might be related to the number of palindromes of hr2.

Key words

Ectropis grisescens / Ectropis obliqua / EoNPV / homologous regions / strain / virulence

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XU Bin, HAN Guangjie, QI Jianhang, LI Chuanming, XU Jian, LU Yurong, LIU Qin. Virulence Difference of Two Strains of EoNPV Isolates to Ectropis obliqua and Ectropis grisescens[J]. Journal of Tea Science. 2021, 41(4): 545-552 https://doi.org/10.13305/j.cnki.jts.2021.04.008

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