Analysis of the Antennal Transcriptome and Olfactory-related Genes in the Agriophara rhombata

LONG Yaqin; LUO Ziwen; WANG Xuesong; LONG Lixue; YU Xiangshuai; LI Jinlong; QU Hao; WANG Yungang; CHEN Linbo

doi:10.13305/j.cnki.jts.20210316.001

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (4) : 553-563. DOI: 10.13305/j.cnki.jts.20210316.001

Research Paper

Analysis of the Antennal Transcriptome and Olfactory-related Genes in the Agriophara rhombata

LONG Yaqin, LUO Ziwen, WANG Xuesong, LONG Lixue, YU Xiangshuai, LI Jinlong, QU Hao, WANG Yungang, CHEN Linbo^*

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Abstract

To identify the olfactory-related genes, the transcriptome analysis of the Agriophara rhombata was performed using Illumina HiSeq 4000. Through sequence alignment, 16 027 unigenes were annotated in NR databases. Totally 11 701 unigenes were annotated in GO database, which can be divided into 3 groups according to their functions: cellular component, molecular function and biological process. Totally 6 047 unigenes were further annotated and divided into 25 functional groups in KOG databases. KEGG pathway analysis shows that 12 009 unigenes were annotated into 283 metabolism pathways. According to the annotation information, 238 candidate olfactory-related genes were obtained including 108 odorant binding protein (OBP) genes, 55 odorant /olfactory receptor (OR) genes, 26 gustatory receptor (GR) genes, 25 ionotropic receptor (IR) genes, 11 chemosensory protein (CSP) genes, 4 sensory neuron membrane protein (SNMP) genes, 4 sensory perception (SP) genes , 4 chemosensory receptor (CR) genes and an odorant degrading enzyme (ODE) gene. It was found that 12 odorant binding protein genes, 9 odorant/olfactory receptor genes, 4 pheromone binding protein genes, 3 gustatory receptor genes, chemosensory protein gene and 1ionotropic receptor gene were differentially expressed in the antenna of Agriophara rhombata. In this study, the identification of candidate olfactory-related genes laid a molecular foundation for further studies on gene function and olfactory perception mechanism of the Agriophara rhombata.

Key words

Agriophara rhombata / antennal transcriptome / genome annotation / high-throughput sequencing / olfaction-related genes

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LONG Yaqin, LUO Ziwen, WANG Xuesong, LONG Lixue, YU Xiangshuai, LI Jinlong, QU Hao, WANG Yungang, CHEN Linbo. Analysis of the Antennal Transcriptome and Olfactory-related Genes in the Agriophara rhombata[J]. Journal of Tea Science. 2021, 41(4): 553-563 https://doi.org/10.13305/j.cnki.jts.20210316.001

References

[1] 陈宗懋, 孙晓玲. 茶树主要病虫害简明识别手册[M]. 北京: 中国农业出版社, 2013: 185-187.
Chen Z M, Sun X L.Concise manual for identification of major pests and diseases of tea plant [M]. Beijing: China Agricultural Press, 2013: 185-187.
[2] 广州军区生产建设兵团六师十三团茶叶试验站. 茶谷蛾研究初报[J]. 中国茶叶, 1974(6): 6-10.
Tea Experimental Station of the 13th Regiment of the 6th Division of Production and Construction Corps of Guangzhou Military Region. A preliminary study on Agriopharar hombata[J]. China Tea, 1974(6): 6-10.
[3] 董祖祥. 一个人和640万条虫的战争[J]. 农药市场信息, 2015, 541(23): 57-59.
Dong Z X.The war between a man and 6.4 million worms[J]. Pesticide Market Information, 2015, 541(23): 57-59.
[4] Zhang S, Pang B P, Zhang L.Novel odorant-binding proteins and their expression patterns in grasshopper, Oedaleus asiaticus[J]. Biochemicaland Biophysical Research Communications, 2015, 460(2): 274-280.
[5] Gadenne C, Barrozo R B, Anton S.Plasticity in insect olfaction: to smell or not to smell[J]. Annual Review of Entomology, 2016, 61(1): 317-333.
[6] 郑海霞, 张耀文, 张仙红, 等. 绿豆象触角转录组及嗅觉相关基因的分析[J]. 昆虫学报, 2018, 61(2) : 168-177.
Zheng H X, Zhang Y W, Zhang X H, et al.Analysis of the antennal transcriptome and olfaction-related genes of Callosobruchus chinensis (Coleoptera: Bruchuidae)[J]. Acta Entomologica Sinica, 2018, 61(2): 168-177.
[7] 杨雪娇, 张蔓, 江宇航, 等. 七星瓢虫触角转录组及嗅觉相关基因分析[J]. 昆虫学报, 2020, 63(6): 717-726.
Yang X J, Zhang M, Jiang Y H, et al.Analysis of the antennal transcriptome and olfaction-related genes of Coccinellaseptempunctata (Coleoptera: Coccinellidae)[J]. Acta Entomologica Sinica, 2020, 63(6): 717-726.
[8] Zhou J J.Odorant-binding proteins in insects[J]. Vitamins & Hormones, 2010, 83: 241-272.
[9] 王菁桢. 两种星天牛嗅觉相关蛋白基因的鉴定及关键气味结合蛋白的功能分析[D]. 北京: 北京林业大学, 2019.
Wang J Z.Identification of olfactory related genes and functional analysis of the key odorant binding protein from two species of Anoplophora [D]. Beijing: Beijing Forestry University, 2019.
[10] 赵磊, 崔宏春, 张林雅, 等. 茶尺蠖普通气味结合蛋白EoblGOBP2与茶树挥发物的结合功能研究[J]. 茶叶科学, 2014, 34(2): 165-171.
Zhao L, Cui H C, Zhang L Y, et al.Molecular binding characterization with tea plant volatiles of a general odorant-bingding protein EoblGOBP2 in the Tea Geometrid, Ectropis oblique Prout (Lepidoptera: Geometridae)[J]. Journal of Tea Science, 2014, 34(2): 165-171.
[11] 马龙. 茶尺蠖化学感受相关基因的克隆与功能研究[D]. 北京: 中国农业科学院, 2016.
Ma L.Molecular and functional characterization of chemosensory genes in Ectropis oblique [D]. Beijing: Chinese Academy of Agricultural Sciences, 2016.
[12] 田振. 苹果蠹蛾信息素结合蛋白的研究及活性气味分子的筛选[D]. 杨凌: 西北农林科技大学, 2016.
Tian Z.Study on cydia pomonella pheromone binding Proteins and screening of potentially active semiochemicals [D]. Yangling: Northwest Agriculture & Forestry University, 2016.
[13] Wang B, Liu Y, Wang G R.Chemosensory genes in the antennal transcriptome of two syrphid species, Episyrphus balteatus and Eupeodes corollae (Diptera: Syrphidae)[J]. BMC Genomics, 2017, 18(1): 586. doi: 10.1186/s12864-017-3939-4.
[14] 王晓双, 唐良德, 吴建辉. 昆虫嗅觉相关蛋白的研究进展[J]. 热带作物学报, 2017, 38(6): 1171-1179.
Wang X S, Tang L D, Wu J H.Research progress on olfactory proteins of insects[J]. Chinese Journal of Tropical Crops, 2017, 38(6): 1171-1179.
[15] Jia X J, Wang H X, Yan Z G, et al.Antennal transcriptome and differential expression of olfactory genes in the yellow peach moth, Conogethes punctiferalis (Lepidoptera: Crambidae)[J]. Scientific Reports, 2016, 6: 29067. doi: 10.1038/srep29067.
[16] 杨爽, 赵慧婷, 徐凯, 等. 大蜡螟触角转录组及嗅觉相关基因分析[J]. 应用昆虫学报, 2019, 56(6): 1279-1291.
Yang S, Zhao H T, Xu K, et al.Analysis of the antennal transcriptome and olfaction-related genes of the greater wax moth Galleria mellonella (Lepidoptera: Pyralidae)[J]. Chinese Journal of Applied Entomology, 2019, 56(6): 1279-1291.
[17] Zhang T, Coates B S, Ge X, et al.Male-and female-biased gene expression of olfactory-related genes in the antennae of asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Crambidae)[J]. Plos One, 2015, 10(6): e0128550. doi:10.1371/journal. pone.0128550.
[18] 黄钧鸿, 张铭淇, 冯启理, 等. 草地贪夜蛾与斜纹夜蛾嗅觉味觉相关基因的比较分析[J]. 环境昆虫学报, 2019, 41(5): 937-946.
Huang J H, Zhang M Q, Feng Q L, et al.Comparison of olfactory and gustatory genes between Spodoptera frugiperda and Spodoptera litura[J]. Journal of Environmental Entomology, 2019, 41(5): 937-946.
[19] 胡佳萌, 徐丹萍, 卓志航, 等. 云斑天牛成虫触角转录组及嗅觉相关基因分析[J]. 应用昆虫学报, 2019, 56(5): 1037-1047.
Hu J M, Xu D P, Zhuo Z H, et al.Analysis of antennal transcriptome and olfaction-related genes of adult Batocera horsfieldi (Hope)[J]. Chinese Journal of Applied Entomology, 2019, 56(5): 1037-1047.
[20] Grabherr M G, Haas B J, Yassour M, et al.Full-length transcriptome assembly from RNA-Seq data without a reference genome[J]. Nature Biotechnology, 2011, 29(7): 644-652. doi: 10.1038/nbt.1883.
[21] Li B, Dewey C.RSEM: Accurate transcript quantification from RNA-Seq data with or without a reference genome[J]. BMC Bioinformatics, 2011, 12: 323. doi: 10.1186/1471-2105-12-323.
[22] Robinson M D, McCarthy D J, Smyth G K. A bioconductor package for differential expression analysis of digital gene expression data[J]. Bioinformatics, 2010, 26: 139-140. doi: 10.1093/bioinformatics/btp616.
[23] 杨海博, 胡镇杰, 李定旭, 等. 悬铃木方翅网蝽触角转录组及嗅觉相关基因分析[J]. 农业生物技术学报, 2018, 26(12): 2109-2120.
Yang H B, Hu Z J, Li D X, et al.Analysis of the antennal transcriptome and olfactory-related genes of the Sycamore Lace Bug (Corythucha ciliata)[J]. Journal of Agricultural Biotechnology, 2018, 26(12): 2109-2120.
[24] 张新, 陈成聪, 杜琴, 等. 茶尺蠖丝氨酸蛋白酶基因EoSP1的克隆、时空表达及对饥饿的表达响应[J]. 茶叶科学, 2019, 39(6): 669-680.
Zhang X, Chen C C, Du Q, et al.Cloning and expression analysis of serine protease EoSP1 in Tea Geometrid (Ectropis obliqua) and its response to starvation[J]. Journal of Tea Science, 2019, 39(6): 669-680.
[25] 刘新, 陈红平, 王国庆. 中国茶叶质量安全40年[J]. 中国茶叶, 2019, 41(12): 1-9.
Liu X, Wang G Q, Chen H P, et al.The development of tea quality and safety in China over the past 40 years[J]. China Tea, 2019, 41(12): 1-9.
[26] 王兴春, 谭河林, 陈钊, 等. 基于RNA-Seq技术的连翘转录组组装与分析及SSR分子标记的开发[J]. 中国科学: 生命科学, 2015, 45(3): 301-310.
Wang X C, Tan H L, Chen Z, et al.Assembly and characterization of the transcriptome and developmentof SSR markers in Forsythia suspense based on RNA-Seq technology[J]. Scientia Sinica (Vitae), 2015, 45(3): 301-310.
[27] 谢翠琴, 聂红毅, 苏松坤. 昆虫触角转录组研究进展[J]. 应用昆虫学报, 2016, 53(6): 1157-1164.
Xie C Q, Nie H Y, Su S K.Advances in research on antennal transcriptome in insects[J]. Chinese Journal of Applied Entomology, 2016, 53(6): 1157-1164.
[28] Allen J E, Wanner K W.Asian corn borer pheromone binding protein 3 a candidate for evolving specificity to the 12-tetradecenyl acetate sex pheromone[J]. Insect Biochemistry & Molecular Biology, 2011, 41(3): 141-149.
[29] 任倩倩, 庄明珠, 蔡晓明, 等. 小贯小绿叶蝉取食诱导抗、感茶树品种挥发物的释放[J]. 茶叶科学, 2020, 40(6): 795-806.
Ren Q Q, Zhuang M Z, Cai X M, et al.The release of volatiles in resistant and susceptible tea cultivars under Empoasca Onukii feeding[J]. Journal of Tea Science, 2020, 40(6): 795-806.
[30] Leal W S.Odorant reception in insects: roles of receptors, binding proteins, and degrading enzymes[J]. Annual Review of Entomology, 2013, 58(1): 373-391.