Molecular Identification and Virulence Evaluation of an Entomopathogenic Fungus Isolated from the Cadaver of Sympiezomias citri

WANG Dingfeng; LI Liangde; LI Huiling; ZHANG Hui; WANG Qingsen; WU Guangyuan

doi:10.13305/j.cnki.jts.2018.03.012

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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (3) : 313-318. DOI: 10.13305/j.cnki.jts.2018.03.012

Molecular Identification and Virulence Evaluation of an Entomopathogenic Fungus Isolated from the Cadaver of Sympiezomias citri

WANG Dingfeng, LI Liangde, LI Huiling, ZHANG Hui, WANG Qingsen, WU Guangyuan^*

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Abstract

The taxonomic status and biocontrol potential of an entomopathogenic fungus that isolated from the cadaver of Sympiezomias citri were investigated. Amplification, sequencing, and system evolution tree construction of the nuclear ribosomal internal transcribed spacer (rDNA-ITS) and intergenic Bloc region fragments (Bloc) of the fungus Bbr4941, and the bioassay against the adult of S. citri were carried out. The results showed that the entomopathogenic fungus was identified as Beauveria brongniartii. When the adults of S. citri were dealt with Bbr4941 suspension (5.0×10⁷spore·mL^-1), the corrected accumulative mortality was 100% and LT₅₀ value was 3.916βd after 7 days. Our results showed that Bbr4941 had great potential for the biocontrol of S. citri.

Key words

Sympiezomias citri / Bloc region / entomopathogenic fungi / rDNA-ITS / virulence

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WANG Dingfeng, LI Liangde, LI Huiling, ZHANG Hui, WANG Qingsen, WU Guangyuan. Molecular Identification and Virulence Evaluation of an Entomopathogenic Fungus Isolated from the Cadaver of Sympiezomias citri[J]. Journal of Tea Science. 2018, 38(3): 313-318 https://doi.org/10.13305/j.cnki.jts.2018.03.012

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