Isolation and Identification of highly pathogenic Beauveria bassiana against Thrips

WANG Jun-ping; ZHENG Chang-ying

doi:10.13305/j.cnki.jts.2011.04.010

PDF(469 KB)

Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (4) : 295-299. DOI: 10.13305/j.cnki.jts.2011.04.010

Isolation and Identification of highly pathogenic Beauveria bassiana against Thrips

WANG Jun-ping, ZHENG Chang-ying^*

Author information +

History +

Abstract

12 different entomopathogenic fungi isolated from diseased Drosicha corpulenta Kuwana showed high virulence with 70%~90% mortality. CYT10 strain was identified based on the morphological and molecular characteristics. Conidiogenous cells were short-globose to flask-shaped and the conidiophore elongated in a long zig-zag extension after each conidium is produced. The result of Blast search indicated that the ITS and EF1-α sequence of CYT10 showed a high similarity with those of other strain of Beauveria bassiana (>95%). Phylogenetic analysis showed that it was closely related to ARSEF-1185 strain, which was belonging to B. bassiana. CYT10 strain was classified as a member of the species B. bassiana based on the morphological characteristics and analysis of DNA sequences.

Key words

Beauveria bassiana / EF1-a / morphological characteristics / rDNA-ITS / thrip

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

WANG Jun-ping, ZHENG Chang-ying. Isolation and Identification of highly pathogenic Beauveria bassiana against Thrips[J]. Journal of Tea Science. 2011, 31(4): 295-299 https://doi.org/10.13305/j.cnki.jts.2011.04.010

References

[1] Todorova SI, Cloutier C, Cote JC.Pathogenicity of six isolates of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycotina, Hyphomycetes) to Perillus cioculatus (F) (Henm, Pentatomidae)[J]. Appl Entomol, 2002, 126(4): 182-185.
[2] Rehner SA, Buckley E.A Beauveria phylogeny inferred from nuclear ITS and EF1-a sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs[J]. Mycologia, 2005, 97(1): 84-98.
[3] 李荣森, 罗绍彬, 张用梅, 等. 微生物防治害虫[M]. 北京: 科学出版社, 1983: 27-45.
[4] 冯明祥, 姜瑞德. 崂山茶区茶树害虫的发生及生态控制方法探讨[J]. 中国茶叶, 2010, 32(2): 10-12.
[5] 谢振伦. 茶黄蓟马的生物学特性与防治[J]. 茶叶科学, 1987, 7(2): 29-34.
[6] 张衍炽. 茶黄蓟马的发生与防治[J]. 茶叶科学与技术, 2008(1): 49.
[7] 姚雍静, 卢天国, 郭灿, 等. 植物浸提液防治茶棍蓟马药效试验[J]. 茶叶科学, 2010, 38(3): 26-28.
[8] 王国华, 夏绍湄, 韩宝瑜. 贵州茶园害虫区系考查及优势种演替趋势分析[J]. 安徽农业大学学报, 2010, 37(3): 411-416.
[9] 王定锋, 吴光远, 王庆森, 等. 茶卷叶蛾寄生真菌的分离、鉴定及毒力测定[J]. 福建农业学报, 2010, 25(3): 345-349.
[10] 殷坤山. 茶树害虫的三种主要病原微生物(续)[J]. 中国茶叶, 2006, 28(5): 28-29.
[11] 方卫国, 杨星勇, 张永军, 等. 真菌核酸的一种快速提取方法[J]. 应用与环境生物学报, 2002, 89(3): 305-307.
[12] 中国科学院微生物研究所. 常见及常用真菌[M]. 北京: 科学出版社, 1978.
[13] Sevim A, Demir I, Demirbağ Z.Molecular characterization and virulence of Beauveria spp.from the pine processionary moth, Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae)[J]. Mycopathologia, 2010a, 170(4): 269-77.
[14] Sevim A, Demir I, Hofte M, et al. Isolation and characterization of entomopathogenic fungi from hazelnut-growing region of Turkey[J]. BioControl, 2010b, 55(2): 279-297.