Study on the Differences of Leaf Color and Volatiles of Different Insect-resistance Tea Cultivars

SUN Yue; LIU Mengyue; GAO Chenxi; WU Quanjin; CAO Shixian; YU Shuntian; CHEN Zhidan; JIN Shan; SUN Weijiang

doi:10.13305/j.cnki.jts.2023.04.005

PDF(2465 KB)

Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (4) : 525-543. DOI: 10.13305/j.cnki.jts.2023.04.005

Research Paper

Study on the Differences of Leaf Color and Volatiles of Different Insect-resistance Tea Cultivars

SUN Yue^1,3, LIU Mengyue^2,3, GAO Chenxi^1,3, WU Quanjin⁴, CAO Shixian⁵, YU Shuntian⁵, CHEN Zhidan^2,3, JIN Shan^1,*, SUN Weijiang^1,3,*

Author information +

History +

Abstract

In order to explore the differences in leaf color and volatiles of different insect-resistant tea cultivars, a two-year field population density survey was conducted on 11 tea cultivars. Through the indoor incubation test of field branches and the indoor life parameter determination test, the reliability of field population density as the resistance grading standard was proved. The color difference and wax content per unit leaf area of leaves were determined, and the volatiles of new shoots of one bud and two leaves that were not harmed by pests and diseases were detected by gas chromatography-mass spectrometry (GC-MS) in order to screen the differences among different insect-resistant tea cultivars. The results show that, M. onukii preferred tea cultivars with darker, lighter yellow leaves. D minowai preferred tea cultivars with brighter, more saturated colors and a higher yellow color, and D minowai placed more emphasis on the color of the leaf back. In terms of volatiles, the population densities of M. onukii were significantly and positively correlated with the relative content of linalool and negatively correlated with the relative contents of nonanal and dodecane. Population densities of D. minowai were significantly and positively correlated with the relative levels of dodecane and phenylethanol. The effects of dodecane on M. onukii and D. minowai were opposite, and the same trend was observed for the relationship between wax and population density in tea leaves, suggesting that the same volatile substance may have different reactions to different insects.

Key words

Dendrothrips minowai / leaf color / Matsumurasca onukii / resistance / volatile components / tea plant

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

SUN Yue, LIU Mengyue, GAO Chenxi, WU Quanjin, CAO Shixian, YU Shuntian, CHEN Zhidan, JIN Shan, SUN Weijiang. Study on the Differences of Leaf Color and Volatiles of Different Insect-resistance Tea Cultivars[J]. Journal of Tea Science. 2023, 43(4): 525-543 https://doi.org/10.13305/j.cnki.jts.2023.04.005

References

[1] 任倩倩. 抗、感茶树品种对茶小绿叶蝉取食诱导的防御反应[D]. 福州: 福建农林大学, 2020.
Ren Q Q.The defense strategies of resistant and susceptible tea cultivars in response to Empoasca onukii feeding [D]. Fuzhou: Fujian Agriculture and Forestry University, 2020.
[2] Chen L L, Yuan P, Pozsgai G, et al.The impact of cover crops on the predatory mite Anystis baccarum (Acari, Anystidae) and the leafhopper pest Empoasca onukii (Hemiptera, Cicadellidae) in a tea plantation[J]. Pest Management Science, 2019, 75(12): 3371-3380.
[3] 唐美君, 肖强. 茶树病虫及天敌图谱[M]. 北京: 中国农业出版社, 2018.
Tang M J, Xiao Q.Colored pitiorial handbook of tea plant pests and natural enemies [M]. Beijing: China Agriculture Press, 2018.
[4] Affeldt H A, Thimijan R W, Smith F F, et al.Response of the green-house whitefly (Homoptera: Aleyrodidae) and the vegetable leafminer (Diptera: Afromyzidae) to photospectra[J]. Journal of Economic Entomology, 1983, 76(6): 1405-1409.
[5] Snoeren T A L, Peter W, Jong D E, et al. Ecogenomic approach to the role of herbivore-induced plant volatiles in community ecology[J]. Journal of Ecology, 2007, 95(1): 17-26.
[6] Kessler A, Baldwin I T.Defensive function of herbivore-induced plant volatile emissions in nature[J]. Science, 2001, 291(5511): 2141-2144.
[7] Shulaev V, Silverman P, Raskin I.Airborne signalling by methyl salicylate in plant pathogen resistance[J]. Nature, 1997, 385(6618): 718-721.
[8] 蔡晓明. 三种茶树害虫诱导茶树挥发物的释放规律[D]. 北京: 中国农业科学院, 2009.
Cai X M.The emission of tea plant volatiles induced by three herbivore insect pests [D]. Beijing: Chinese Academy of Agricultural Sciences, 2009.
[9] 钮羽群, 王梦馨, 崔林, 等. 迷迭香挥发物不同组合对假眼小绿叶蝉行为的调控[J]. 生态学报, 2015, 35(7): 2380-2387.
Niu Y Q, Wang M X, Cui L, et al.Control of the tea green leafhopper via volatile compounds of rosemary: the potential for further development of a Push-Pull strategy[J]. Acta Ecologica Sinica, 2015, 35(7): 2380-2387.
[10] 王梦馨, 李辉仙, 武文竹, 等. 假眼小绿叶蝉对茶梢挥发物的行为反应[J]. 应用昆虫学报, 2016, 53(3): 507-515.
Wang M X, Li H X, Wu W Z, et al.Behavioral responses of Empoasca vitis Göthe to volatiles from tea shoots[J]. Chinese Journal of Applied Entomology, 2016, 53(3): 507-515.
[11] 王蔚, 吴满容, 张思校, 等. 茶小绿叶蝉在福建省茶树品种上的选择机制初探[J]. 河南农业科学, 2016, 45(4): 80-84.
Wang W, Wu M R, Zhang S X, et al.Preliminary study on selection mechanism of Empoasca onukii on tea varieties in Fujian Province[J]. Journal of Henan Agricultural Sciences, 2016, 45(4): 80-84.
[12] 金珊. 不同茶树品种抗假眼小绿叶蝉机理研究[D]. 咸阳: 西北农林科技大学, 2012.
Jin S.Resistance mechanisms of tea plant cultivars to tea green leafhopper [D]. Xianyang: Northwest A&F University, 2012.
[13] 李红莉, 崔宏春, 黄海涛, 等. 联苯菊酯等4种杀虫剂对茶小贯小绿叶蝉的田间防效[J]. 浙江农业科学, 2021, 62(3): 565-566.
Li H L, Cui H C, Huang H T, et al.Field control effect of 4 insecticides such as bifenthrin on Empoasca onukii[J]. Journal of Zhejiang Agricultural Sciences, 2021, 62(3): 565-566.
[14] 施龙清. 福建茶小绿叶蝉种类鉴定及其对光的行为响应[D]. 福州: 福建农林大学, 2016.
Shi L Q.Identification of the tea green leafhopper in Fujian, China, and its behavioral responses to varying light conditions [D]. Fuzhou: Fujian Agriculture and Forestry University, 2016.
[15] 刘勇, 陈巨莲, 程登发. 不同小麦品种(系)叶片表面蜡质对两种麦蚜取食的影响[J]. 应用生态学报, 2007(8): 1785-1788.
Liu Y, Cheng J L, Cheng D F.Effects of wheat leaf surface waxes on the feeding of two wheat aphid species[J]. Chinese Journal of Applied Ecology, 2007(8): 1785-1788.
[16] 吴少玲, 王治会, 商虎, 等. 不同堆青时间对白茶风味品质的影响[J]. 茶叶科学, 2023, 43(1): 78-90.
Wu S L, Wang Z H, Shang H, et al.Effect of pile-up processing duration on flavor quality of white tea[J]. Journal of Tea Science, 2023, 43(1): 78-90.
[17] 石碧滢, 周承哲, 田采云, 等. 不同贮藏时间白牡丹茶风味品质差异分析[J/OL]. 食品科学, 2022: 1-23 [2023-01-12]. http://kns.cnki.net/kcms/detail/11.2206.TS.220221125.1537.012.html.
Shi B Y, Zhou C Z, Tian C Y, et al. Analysis of flavor quality differences between white peony tea with different storage time [J/OL]. Food Science, 2022: 1-23 [2022-12-11]. http://kns.cnki.net/kcms/detail/11.2206.TS.20221125.1537.012.html.
[18] 孙悦, 吴俊, 韦朝领, 等. 抗小贯松村叶蝉和茶棍蓟马的茶树种质筛选及其抗性相关因素分析[J]. 茶叶科学, 2022, 42(5): 689-704.
Sun Y, Wu J, Wei C L, et al.Screening of tea germplasm resistant to Matsumurasca Onukii and Dendrothrips minowai Priesner and analysis of resistance-related factors[J]. Journal of Tea Science, 2022, 42(5):689-704.
[19] 杨春, 孟泽洪, 李帅, 等. 贵州茶园茶棍蓟马和小贯小绿叶蝉种群动态及主栽茶树品种寄主抗性[J]. 南方农业学报, 2021, 52(3): 671-681.
Yang C, Meng Z H, Li S, et al.Population dynamics of Dendrothrips minowai Priesner and Empoasca onukii Matsuda and host resistance of major tea varieties in Guizhou[J]. Journal of Southern Agriculture, 2021, 52(3): 671-681.
[20] Sun Q Y, Chen W L, Ge C M, et al.Correlation of tea green leafhopper occurrence with leaf structure and biochemical components in different tea cultivars[J]. International Journal of Pest Management, 2023, 69(2): 109-119.
[21] 刘丰静, 冉伟, 李喜旺, 等. 小贯小绿叶蝉在5个茶树品种(系)上的蜜露排泄量与茶树叶片结构比较[J]. 茶叶科学, 2020, 40(5): 625-631.
Liu F J, Ran W, Li X W, et al.The comparison of leaf structures and Matsumurasca onukii honeydew excretion among five tea cultivars (strains)[J]. Journal of Tea Science, 2020, 40(5): 625-631.
[22] 金珊, 孙晓玲, 张新忠, 等. 8个茶树品种生化成分分析及抗性成分的初步鉴定[J]. 应用昆虫学报, 2016, 53(3): 516-527.
Jin S, Sun X L, Zhang X Z, et al.Chemical analysis of 8 tea cultivars with different levels of resistance to Empoasca vitis Göthe and a preliminary identification of the chemical basis of this resistance[J]. Chinese Journal of Applied Entomology, 2016, 53(3): 516-527.
[23] 郑雨婷, 王梦馨, 崔林, 等. 基于EPG技术分析茶树品种对茶小绿叶蝉的抗性及其相关的抗性物质[J]. 生态学报, 2017, 37(23): 8015-8028.
Zheng Y T, Wang M X, Cui L, et al.Resistance of tea cultivars to the tea green leafhopper analyzed by EPG technique and their resistance-related substances[J]. Acta Ecologica Sinica, 2017, 37(23): 8015-8028.
[24] Liao Y Y, Yu Z M, Liu X Y, et al.Effect of major tea insect attack on formation of quality-related nonvolatile specialized metabolites in tea (Camellia sinensis) leaves[J]. Journal of Agricultural and Food Chemistry, 2019, 67(24): 6716-6724.
[25] Wang M X, Han S J, Wu Y Q, et al.Tea green leafhopper-induced synomone attracts the egg parasitoids, mymarids to suppress the leafhopper[J]. Pest Management Science, 2023. doi: 10.1002/ps.7563.
[26] 边磊, 孙晓玲, 高宇, 等. 昆虫光趋性机理及其应用进展[J]. 应用昆虫学报, 2012, 49(6): 1677-1686.
Bian L, Sun X L, Gao Y, et al.Research on the light tropism of insects and the progress in application[J]. Chinese Journal of Applied Entomology, 2012, 49(6): 1677-1686.
[27] Knolhoff L M, Heckel D G.Behavioral assays for studies of host plant choice and adaptation in herbivorous insects[J]. Annual Review of Entomology, 2014, 59(1): 263-278.
[28] 林金丽, 韩宝瑜, 周孝贵, 等. 色彩对茶园昆虫的引诱力[J]. 生态学报, 2009, 29(8): 4303-4316.
Lin J L, Han B Y, Zhou X G, et al.The attraction of color to insects in tea garden[J]. Acta Ecologica Sinica, 2009, 29(8): 4303-4316.
[29] Bian L, Yang P X, Yao Y J, et al.Effect of trap color, height, and orientation on the capture of yellow and stick tea thrips (Thysanoptera: Thripidae) and nontarget insects in tea gardens[J]. Journal of Economic Entomology, 2016, 109(3): 1241-1248.
[30] Mohammad H A T, Nazrul I M, Zhang Y, et al. Leaf morphological characters can be a factor for intra-varietal preference of whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) among eggplant varieties[J]. Plos One, 2016, 11(4): e0153880. doi: 10.1371/journal.pone.0153880.
[31] 曹凤勤, 刘万学, 万方浩, 等. 寄主挥发物、叶色在B型烟粉虱寄主选择中的作用[J]. 应用昆虫学报, 2008, 45(3): 431-436.
Cao F Q, Liu W X, Wan F H, et al.Behavior selection of Bemisia tabaci B-biotype to different host plants and colors[J]. Chinese Journal of Applied Entomology, 2008, 45(3): 431-436.
[32] 王琛, 朱文静, 符悦冠, 等. 茶黄蓟马嗜好颜色筛选及监测效果测定[J]. 环境昆虫学报, 2015, 37(1): 107-115.
Wang C, Zhu W J, Fu Y G, et al.Screening of preferred color and field evalution for monitoring Scirtothrips dorsalis Hood (Thysanoptera: Thripidae)[J]. Journal of Environmental Entomology, 2015, 37(1): 107-115.
[33] 乔利, 金银利, 蒋月丽, 等. 光谱对小贯小绿叶蝉趋光行为的影响[J]. 环境昆虫学报, 2022, 44(5): 1197-1204.
Qiao L, Jin Y L, Jiang Y L, et al.Effects of spectra on phototactic behavior of the Empoasca onukii Matsuda[J]. Journal of Environmental Entomology, 2022, 44(5): 1197-1204.
[34] 叶火香, 韩善捷, 韩宝瑜. 假眼小绿叶蝉趋色性及茶梢信息物质强化其趋色性的效应[J]. 安徽农业大学学报, 2015, 42(6): 868-872.
Ye H X, Han S J, Han B Y.Phototaxis of tea green leafhopper and strengthening effect of infochemicals from tea shoots on its phototaxis[J]. Journal of Anhui Agricultural University, 2015, 42(6): 868-872.
[35] Zeng L T, Watanabe N, Yang Z Y.Understanding the biosyntheses and stress response mechanisms of aroma compounds in tea (Camellia sinensis) to safely and effectively improve tea aroma[J]. Critical Reviews in Food Science and Nutrition, 2019, 59(14): 2321-2334.
[36] 张冬桃, 孙君, 叶乃兴, 等. 茶树萜烯类香气物质合成相关酶研究进展[J]. 茶叶学报, 2015, 56(2): 68-79.
Zhang D T, Sun J, Ye N X, et al.Research progress of enzymes associated with terpene synthesis in Camellia sinensis[J]. Acta Tea Sinica, 2015, 56(2): 68-79.
[37] 韩宝瑜. 茶树-茶蚜-捕食、寄生性天敌间定位、取食的物理、化学通讯机制[D]. 北京: 中国农业科学院, 1999.
Han B Y.The physical and chemical communication mechanism of tea plant-tea aphid-predator and parasitoid positioning and feeding [D]. Beijing: Chinese Academy of Agricultural Sciences, 1999.
[38] 赵冬香, 高景林, 陈宗懋, 等. 假眼小绿叶蝉对茶树挥发物的定向行为反应[J]. 华南农业大学学报(自然科学版), 2002, 23(4): 27-29.
Zhao D X, Gao J L, Cheng Z M, et al.Orientation response of Empoasca vitis to tea shoots volatiles[J]. Journal of South China Agricultural University (Natural Science Edition), 2002, 23(4): 27-29.
[39] 金珊, 韩李伟, 叶乃兴, 等. 茶小绿叶蝉危害乌龙茶茶树品种的挥发物分析[J]. 热带作物学报, 2019, 40(3): 576-582.
Jin S, Han L W, Ye N X, et al.Analysis on volatiles of Oolong tea varieties induced by Empoasca sp.[J]. Chinese Journal of Tropical Crops, 2019, 40(3): 576-582.
[40] 任倩倩, 庄明珠, 蔡晓明, 等. 小贯小绿叶蝉取食诱导抗、感茶树品种挥发物的释放[J]. 茶叶科学, 2020, 40(6): 795-806.
Ren Q Q, Zhuang M Z, Cai X M, et al.The release of volatiles in resistant and susceptible tea cultivars under Empoasca onukii feeding[J]. Journal of Tea Science, 2020, 40(6): 795-806.
[41] 张辉, 李慧玲, 王定锋, 等. 茶跗线螨为害程度对福云6号鲜叶芳香物质的影响[J]. 福建农业学报, 2015, 30(9): 896-900.
Zhang H, Li H L, Wang D F, et al.Aromatics in fresh leaves of Fuyun 6 (Camellia sinensis cv.) under Polyphagotarsonemus latus stress[J]. Fujian Journal of Agricultural Sciences, 2015, 30(9): 896-900.
[42] 李晓维, 程江辉, 韩海斌, 等. 植物次生代谢物质对蓟马的行为调控作用及其在蓟马防控中的应用[J]. 昆虫学报, 2022, 65(9): 1222-1246.
Li X W, Cheng J H, Han H B, et al.Behavioral manipulation of the plant secondary metabolites to thrips and their application in thrips management[J]. Acta Entomologica Sinica, 2022, 65(9): 1222-1246.
[43] Teulon D A J, Davidson M M, Hedderley D I, et al. 4-pyridyl carbonyl and related compounds as thrips lures: effectiveness for onion thrips and new zealand flower thrips in field experiments[J]. Journal of Agricultural and Food Chemistry, 2007, 55(15): 6198-6205.
[44] 刘莹, 吕东, 陈立, 等. 圆柏大痣小蜂对祁连圆柏挥发物的触角电位和行为反应[J]. 生态学报, 2023, 43(9): 3828-3839.
Liu Y, Lü D, Chen L, et al.Electroant-ennagram and responses of Megastigmus sabinae to volatiles of Sabina przewalskii[J]. Acta Ecologica Sinica, 2023, 43(9): 3828-3839.
[45] 陈旭鹏. 榆木蠹蛾三种寄主树干挥发物分析及植源性引诱剂的筛选[D]. 晋中: 山西农业大学, 2016.
Cheng X P.Identification of volatiles from three host plants attractive for Holcocerus vicarius Walker and the development of plant volatile attractant [D]. Jinzhong: Shanxi Agricultural University, 2016.
[46] 孙廷哲, 岂泽华, 梁可欣, 等. 蚜害茶树挥发物组分变化的聚类分析[J]. 植物学报, 2021, 56(4): 422-432.
Sun T Z, Qi Z H, Liang K X, et al.Clustering analysis of volatile components from the tea plants infested by tea aphid (Toxotera aurantia)[J]. Chinese Bulletin of Botany, 2021, 56(4): 422-432.