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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (3): 310-324.doi: 10.13305/j.cnki.jts.2023.03.001

• Research Paper • Previous Articles     Next Articles

Genetic Analysis and Marker Development for Wuyi Tea (Camellia sinensis, Synonym: Thea bohea L.) Based on GBS-SNP

LI Li1,3, LUO Shengcai2, WANG Feiquan1,3, LI Xiangru1,4, FENG Hua1,3, SHI Yutao1,3, YE Jianghua1,3, LIU Fei1, ZHAO Jialin1, LI Shuying1, ZHANG Bo1,3,*   

  1. 1. Wuyi University College of Tea and Food, Nanping 354300, China;
    2. Agricultural and Rural Bureau in Wuyishan, Nanping 354300, China;
    3. Tea Science Research Institute of Wuyi University, Nanping 354300, China;
    4. Fujian Agriculture and Forestry University, College of Horticulture, Fuzhou 350002, China
  • Received:2023-02-24 Revised:2023-03-24 Online:2023-06-15 Published:2023-06-29

Abstract: In order to understand the genetic diversity background of Wuyi tea (Camellia sinensis, Synonym: Thea bohea L.), 126 Wuyi tea cultivars/strains and 223 elite tea cultivars/strains (a total of 349 tea germplasm resources) from 12 different regions were collected. Genotyping by sequencing (GBS) technology was used to screen 973 high-quality core SNPs for genetic diversity and background analysis. Model-based structure (Structure), phylogenetic tree construction (NJ-tree) and principal component analysis (PCA) show that the 349 tea resources could be divided into 5 subgroups, and the clustering of subgroups was mainly based on the genetic relationship between tea resources, rather than morphological characteristics such as tree type or leaf shape. Gene flow analysis shows that Wuyi tea might have spread from southern Fujian Province to Wuyi Mountain in northern Fujian Province and from Wuyi Mountain to Zhejiang Province. Genetic similarity analysis shows that among 349 tea cultivars/strains, 136 pairs of cultivars/strains had genetic similarity greater than 0.9, among which 26 pairs involved Wuyi tea. Based on the results of gene flow and genetic similarity, the genetic relationship and background of some representative and controversial Wuyi tea were discussed and analyzed. Furthermore, through the discernibility analysis of pairwise comparison, 21 SNPs were selected from 973 SNP markers that can 100% identify 349 tea cultivars/strains, among which 18 SNPs could 100% identify 126 Wuyi tea cultivars/strains. Genetic fingerprints were established and identification primers were developed. These results provided valuable information for the management and breeding of Wuyi tea in the future.

Key words: Wuyi tea, GBS, genetic similarity, gene flow, genetic fingerprints, identification primer

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