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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (6): 655-662.doi: 10.13305/j.cnki.jts.2016.06.013

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Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ42 in vivo and in vitro

ZHANG Jing1, HUANG Jian'an1,2,3, CAI Shuxian1, YI Xiaoqin1, LIU Jianjun1, WANG Yingzi1, TIAN Lili1, LIU Zhonghua1,2,3,*   

  1. 1. Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, China;
    2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    3. Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Changsha 410128, China
  • Received:2016-08-08 Online:2016-12-15 Published:2019-08-26

Abstract: In this study, amyloid β-protein Aβ42 was incubated with EGCG, TF, TF-3-G, TF-3'-G, TF-DG at the ratio of 1:1 and 1:5 under simulated physiological condition. The contents of β-sheets after incubation were tested by thioflavin T fluorometric assay. It was found that EGCG and theaflavins could suppress the formation of β-sheet structure, thereby inhibiting aggregations of Aβ42. In addition, an SH-SY5Y cell damage model was established and MTT assay was used to examine cell viabilities when 20 μmol·L-142 was incubated with different concentrations of EGCG and theaflavins (10, 50, 100 μmol·L-1). Intracellular reactive oxygen species (ROS) levels, antioxidant enzyme activities (GSH-Px) and MDA levels were also measured. The results showed EGCG and theaflavins treatments could alleviate the Aβ42 induced oxidative damage to neural cells by reducing ROS and increasing the activity of GSH-Px. To study the in vivo protective effects of EGCG and theaflavins, SAMP8 mice were treated with 10 mg·kg-1·d-1 EGCG or theaflavins respectively by gavage for 10 weeks, and the contents of Aβ42 and AGEs in mouse serum were determined. The results showed that EGCG and theaflavins significantly decreased the contents of Aβ42 and AGEs in SAMP8 serum. In summary, EGCG and theaflavins could inhibit the aggregations of Aβ42 and reduce oxidative injury induced by Aβ42 in neural cells, thereby exerting protective effects on AD.

Key words: EGCG, theaflavins, amyloid-β1-42, advanced glycation end products, SH-SY5Y cells, SAMP8 mice

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