Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-&#x003b2; 1-42 and Inhibit the Level of A&#x003b2;42 in vivo and in vitro

ZHANG Jing; HUANG Jian'an; CAI Shuxian; YI Xiaoqin; LIU Jianjun; WANG Yingzi; TIAN Lili; LIU Zhonghua

doi:10.13305/j.cnki.jts.2016.06.013

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Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (6) : 655-662. DOI: 10.13305/j.cnki.jts.2016.06.013

Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ₄₂ in vivo and in vitro

ZHANG Jing¹, HUANG Jian'an^1,2,3, CAI Shuxian¹, YI Xiaoqin¹, LIU Jianjun¹, WANG Yingzi¹, TIAN Lili¹, LIU Zhonghua^1,2,3,*

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Abstract

In this study, amyloid β-protein Aβ₄₂ was incubated with EGCG, TF, TF-3-G, TF-3'-G, TF-DG at the ratio of 1:1 and 1:5 under simulated physiological condition. The contents of β-sheets after incubation were tested by thioflavin T fluorometric assay. It was found that EGCG and theaflavins could suppress the formation of β-sheet structure, thereby inhibiting aggregations of Aβ₄₂. In addition, an SH-SY5Y cell damage model was established and MTT assay was used to examine cell viabilities when 20 μmol·L^-1 Aβ₄₂ was incubated with different concentrations of EGCG and theaflavins (10, 50, 100 μmol·L^-1). Intracellular reactive oxygen species (ROS) levels, antioxidant enzyme activities (GSH-Px) and MDA levels were also measured. The results showed EGCG and theaflavins treatments could alleviate the Aβ₄₂ induced oxidative damage to neural cells by reducing ROS and increasing the activity of GSH-Px. To study the in vivo protective effects of EGCG and theaflavins, SAMP8 mice were treated with 10 mg·kg-1·d^-1 EGCG or theaflavins respectively by gavage for 10 weeks, and the contents of Aβ₄₂ and AGEs in mouse serum were determined. The results showed that EGCG and theaflavins significantly decreased the contents of Aβ₄₂ and AGEs in SAMP8 serum. In summary, EGCG and theaflavins could inhibit the aggregations of Aβ₄₂ and reduce oxidative injury induced by Aβ₄₂ in neural cells, thereby exerting protective effects on AD.

Key words

advanced glycation end products / amyloid-β1-42 / EGCG / SAMP8 mice / SH-SY5Y cells / theaflavins

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ZHANG Jing, HUANG Jian'an, CAI Shuxian, YI Xiaoqin, LIU Jianjun, WANG Yingzi, TIAN Lili, LIU Zhonghua. Theaflavins and EGCG Protect SH-SY5Y Cells from Oxidative Damage Induced by Amyloid-β 1-42 and Inhibit the Level of Aβ₄₂ in vivo and in vitro[J]. Journal of Tea Science. 2016, 36(6): 655-662 https://doi.org/10.13305/j.cnki.jts.2016.06.013

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