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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (5): 731-739.doi: 10.13305/j.cnki.jts.2022.05.003

• Research Paper • Previous Articles     Next Articles

Preparation of Nanoparticules with Chitosan Complexed β-lactoglobulin Loaded EGCG and their Effects on Blood Glucose in Diabetic Mice

CHEN Ke1,2, WANG Yuanzhu1,2, YANG Xiaoying1,3, ZHANG Dongying1,3, ZHU Qiangqiang1,2,*   

  1. 1. Key Laboratory of Pu-erh Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China;
    2. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
    3. College of Science, Yunnan Agricultural University, Kunming 650201, China
  • Received:2022-04-11 Revised:2022-06-20 Online:2022-10-15 Published:2022-10-28

Abstract: As the main bioactive component in tea, EGCG has abundant physiological functions. However, its low stability makes it easy to be degraded and has low bioavailability. In this experiment, carboxymethyl chitosan (CMC), chitosan hydrochloride (CHC), and β-lactoglobulin (β-LG) were used as wall materials to encapsulate EGCG. The microscopic morphology of particles were observed through electron microscope microscopic morphological observation, structural characterization (measurement of particle size, Zeta potential). The entrapment efficiency and simulated gastrointestinal fluid release rate were determined by HPLC. Finally, the activities of nanoparticles were explored by measuring the effect of nanoparticles on blood glucose in diabetic mice. The result shows that the prepared CS-β-LG-EGCG nanoparticles had complete structures, particle size of 10-100 nm, and certain dispersibilities. The entrapment efficiency was greater than 50%, and it had a slow-release effect in intestinal and gastric juices. CS-β-LG-EGCG nanoparticles had no antagonistic effect with insulin. Compared with uncoated EGCG, the sustained-release effect of the particles can slow down the recovery of blood glucose.

Key words: EGCG, chitosan, β-lactoglobulin, simulated gastric juice, diabetes

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