A Study on the Neuroprotective Effects of Combined EGCG and L-Theanine from Tea Leaves

DING Shuqia; XIE Xinya; LIU Zhusheng; LIAO Xianjun; LIU Zhonghua; CAI Shuxian

doi:10.13305/j.cnki.jts.20240918.001

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Journal of Tea Science ›› 2024, Vol. 44 ›› Issue (5) : 779-792. DOI: 10.13305/j.cnki.jts.20240918.001

Research Paper

A Study on the Neuroprotective Effects of Combined EGCG and L-Theanine from Tea Leaves

DING Shuqia^1,3, XIE Xinya^1,3, LIU Zhusheng^2,*, LIAO Xianjun², LIU Zhonghua^1,3, CAI Shuxian^1,3,*

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Abstract

Differentiated neurons need to maintain axonal growth and function in a quiescent state. Previous studies have shown that epigallocatechin gallate (EGCG) and L-theanine can maintain the quiescent state of neurons and have neurorestorative effects, although the specific mechanisms are still unclear. In the Aβ_25-35-induced PC12 cell damage model, combined treatment with EGCG and L-theanine improved cell metabolism and repair capacity, enhanced cell viability and showed a significant synergistic effect. Transcriptomic and network pharmacological analyses indicate that EGCG mainly maintains the quiescent state of cells by inhibiting oxidative stress, regulating fatty acid metabolism, and mitigating amyloid protein toxicity stress. L-theanine promotes axonal growth and regulates neuronal metabolism and synaptic function. The combined application of both compounds results in a broader and milder regulation of cellular networks, reducing cellular stress. This study provided theoretical support for the neuroprotective effects of tea and its value in an aging society.

Key words

L-theanine / EGCG / joint analysis / network pharmacology / neuroprotection / transcriptome

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DING Shuqia, XIE Xinya, LIU Zhusheng, LIAO Xianjun, LIU Zhonghua, CAI Shuxian. A Study on the Neuroprotective Effects of Combined EGCG and L-Theanine from Tea Leaves[J]. Journal of Tea Science. 2024, 44(5): 779-792 https://doi.org/10.13305/j.cnki.jts.20240918.001

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