茶树雌蕊总蛋白质双向电泳分离体系的建立

doi:10.13305/j.cnki.jts.2013.05.012

Abstract

Abstract: In order to establish a proper two-dimensional gel electrophoresis (2-DE) protocol for proteomic study of tea plant pistils, several protein extraction methods, density of SDS-PAGE gel, IEF procedures and pH gradient of IPG strip were compared. The results showed the optimized system includes: total proteins of sample extracted using TCA/acetone method followed with clean up, separating the proteins with 17βcm pH 5~8 IPG strips, 13% SDS-PAGE, and CBB R-250 stain method. About 1β200 spots can be detected, and these proteins mainly distributed in the pH 5~8, MW 14~117βkD range. With good separation of the basic protein, this will be helpful to proteomic research in tea plant.

Key words: Camellia sinensis (L.) O. Kuntze, pistil, protein sample preparation, two-dimensional electrophoresis

CLC Number:

S571.1
Q51

REN Yan, CHEN Xuan, FANG Wan-ping, LI Yao, LI Xing-hui. Establishment of Two-dimensional Electrophoresis System for Pistil Proteome Analysis in Tea Plant[J]. Journal of Tea Science, 2013, 33(5): 420-428.

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Establishment of Two-dimensional Electrophoresis System for Pistil Proteome Analysis in Tea Plant

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