Establishment of Two-dimensional Electrophoresis System for Pistil Proteome Analysis in Tea Plant

REN Yan; CHEN Xuan; FANG Wan-ping; LI Yao; LI Xing-hui

doi:10.13305/j.cnki.jts.2013.05.012

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Journal of Tea Science ›› 2013, Vol. 33 ›› Issue (5) : 420-428. DOI: 10.13305/j.cnki.jts.2013.05.012

Establishment of Two-dimensional Electrophoresis System for Pistil Proteome Analysis in Tea Plant

REN Yan^1,2, CHEN Xuan¹, FANG Wan-ping¹, LI Yao¹, LI Xing-hui^1,2,*

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Abstract

In order to establish a proper two-dimensional gel electrophoresis (2-DE) protocol for proteomic study of tea plant pistils, several protein extraction methods, density of SDS-PAGE gel, IEF procedures and pH gradient of IPG strip were compared. The results showed the optimized system includes: total proteins of sample extracted using TCA/acetone method followed with clean up, separating the proteins with 17βcm pH 5~8 IPG strips, 13% SDS-PAGE, and CBB R-250 stain method. About 1β200 spots can be detected, and these proteins mainly distributed in the pH 5~8, MW 14~117βkD range. With good separation of the basic protein, this will be helpful to proteomic research in tea plant.

Key words

Camellia sinensis (L.) O. Kuntze / pistil / protein sample preparation / two-dimensional electrophoresis

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REN Yan, CHEN Xuan, FANG Wan-ping, LI Yao, LI Xing-hui. Establishment of Two-dimensional Electrophoresis System for Pistil Proteome Analysis in Tea Plant[J]. Journal of Tea Science. 2013, 33(5): 420-428 https://doi.org/10.13305/j.cnki.jts.2013.05.012

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