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Journal of Tea Science ›› 2013, Vol. 33 ›› Issue (3): 221-228.doi: 10.13305/j.cnki.jts.2013.03.008

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Research on Enzymatic Characteristics of Anthocyanin Reductase of Tea Plant [Camellia sinensis (L.) O. Kuntze]

YANG Qin1, ZHAO Lei2, LIU Ya-jun1, LIU Li2, WANG Yun-sheng1, GAO Li-ping1,*, XIA Tao2,*   

  1. 1. School of Biology Science, Anhui Agricultural University, Hefei 230036, China;
    2. Key Lab of Tea Biochemistry and Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei 230036, China
  • Received:2012-12-28 Revised:2013-02-18 Online:2013-06-30 Published:2019-09-04

Abstract: Anthocyanidin reductase (ANR) is a key enzyme in the biosynthetic pathway of proanthocyanidins(PAs), which catalyzes anthocyanidins into the corresponding 2, 3-cis-flavan-3-ols. For researching enzymatic characteristics of the enzyme, this study was carried out to express and purify the protein by prokaryotic expression and Cobalt ion affinity column purification. The optimal conditions of CsANR1 were observed at 40℃ and pH 6.5. The more substrate preference of CsANR1 was showed on cyanidin over delphinidin. Moreover, Cu2+, Co2+, Fe2+, Mn2+, Zn2+ and Hg2+inhibited the enzyme activity and the enzyme activity decreased 50% after storing 15 days.

Key words: tea plant[Camellia sinensis (L.) O. Kuntze], anthocyanin reductase, prokaryotic expression, enzymatic characteristics

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