茶树泛素活化酶基因全长cDNA克隆及序列分析

doi:10.13305/j.cnki.jts.2012.06.011

Abstract

Abstract: The cDNA-AFLP technology was applied to analyze gene expression during periodic albinism process of Anji Baicha. Some transcript-derived fragments (TDFs) were isolated occurring in both the albinistic and re-greening stage leaves. One of them showed a high similarity to ubiquitin-activating enzyme 1 (UBA1) gene. Based on the fragment, the full length of UBA1 gene with 3764bp (GenBank Accession No. JN180299) cDNA was obtained via rapid amplification of cDNA ends (RACE), named Camellia Sinensis UBA1 gene. It contained an open reading frame (ORF) encoding a polypeptide of 1094 amino acid residues with a predicable molecular mass of 121kD. Analysis of the nucleotide sequence and deduced amino acid sequence showed 82%, 81%, 79%, 79%, 77% homology with UBA1 genes from Nicotiana tabacum, Ricinus communis, Oryza sativa subsp. japonica, Triticum aestivum, Arabidopsis thaliana, respectively. Analysis by qRT-PCR showed that the transcript of UBA1 was significantly up-regulated at the albinistic stage to 2.49-fold higher than that at the re-greening stage. This is a key enzyme in the ubiquitin-proteasome mediated protein degradation system. The clone and analysis of the tea plant UBA1 gene establishes a good foundation for further study on the molecular mechanism of periodic albinism in Anji Baicha.

Key words: Camellia sinensis (L.) O. Kuntze, Ubiquitin-Activating Enzyme 1(UBA1), cDNA cloning, sequence analysis

CLC Number:

S571.1
Q52

DENG Ting-ting, WU Yang, LI Juan, LI Yin-hua, HUANG Jian-an, LIU Zhong-hua. Cloning and Sequencing of UBA1 Gene Full-length cDNA from Tea Plant[J]. Journal of Tea Science, 2012, 32(6): 500-508.

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Cloning and Sequencing of UBA1 Gene Full-length cDNA from Tea Plant

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