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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (4): 385-395.doi: 10.13305/j.cnki.jts.2018.04.006

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The Identification and Analysis of Polyphenol Oxidase Gene Family in Tea Plant (Camellia sinensis)

ZENG Zeyuan1, LUO Yong1, ZHENG Chuchu1, LI Juan1,2,3, LI Qin1,2,3, LIN Haiyan1,2,3,*, WANG Kunbo1,2,3,*   

  1. 1. Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, China;
    2. National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Changsha 410128, China;
    3. Collaborative Innovation Center of Utilization of Functional Ingredients from Botanicals, Changsha 410128, China
  • Received:2018-01-26 Revised:2018-03-20 Online:2018-08-15 Published:2019-10-15

Abstract: :Polyphenol oxidase (PPO) is a copper-containing metal enzyme, which plays an important role in tea processing. Five PPO genes were found in the tea genome database: CsPPO1, CsPPO2, CsPPO3, CsPPO4 (GenBank Access. No. GQ129142.1) and CsPPO5. The nucleic acid and amino acid sequences of five PPO genes were analyzed, and the physicochemical properties and structural functions of the PPO genes encoding protein were also predicted by bioinformatics method. The results showed that the full length of the five PPO genes coding sequences (CDS) was between 597βbp and 1β839βbp which could encode 198-612 amino acids. Phylogenetic tree analysis showed that CsPPO1, CsPPO3, CsPPO4 and CsPPO5 had close genetic relationships. The encoded proteins belonged to hydrophilic and unstable lipid binding proteins and had no signal peptides. Irregular curl was the main structural element of protein in which α-helix and β-strand were dispersed throughout the polypeptide chain & CsPPO1, CsPPO2, CsPPO3 might contain one or more transmembrane spirals. The five PPO proteins contained PPO1_DWL (pfam12142) conserved structural domains. It was predicted that the proteins encoded by CsPPO1, CsPPO3, CsPPO4 and CsPPO5 might contain tyrosinase functional domains. Real-time reverse transcription (qRT-PCR) analysis showed that the PPO genes showed different expression patterns among different tea cultivars.

Key words: tea plant, polyphenol oxidase, gene family, sequence analysis

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