Full-length cDNA Cloning and Sequence Analysis of Theanine Synthetase Gene in Camellia Sinensis

LI Juan; DENG Ting-ting; WU Yang; LIU Shuo-qian; LI Qin; LIU Zhong-hua; HUANG Jian-an

doi:10.13305/j.cnki.jts.2011.05.012

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Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (5) : 411-418. DOI: 10.13305/j.cnki.jts.2011.05.012

Full-length cDNA Cloning and Sequence Analysis of Theanine Synthetase Gene in Camellia Sinensis

LI Juan, DENG Ting-ting, WU Yang, LIU Shuo-qian, LI Qin, LIU Zhong-hua^*, HUANG Jian-an^*

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Abstract

The full-length cDNA of theanine synthetase (TS, GenBank accession No.JN226569) gene of Anji white tea was cloned. The cDNA sequence has the full-length of 1503bp, and with an Open Reading Frame of 1071bp. It can encode 356 amino acids. Bioinformatics analysis showed that theanine synthetase has 356 amino acid residues, has a theoretical molecular weight of 39250.3Da and theoretical PI value of 5.79. Bioinformatics prediction showed that this protein is hydrophilic and located not within the transmembrane. There are 26 phosphorylation sites within the polypeptied chain. The signal peptide analysis showed that there is no signal peptide and no winded helix domain present within the sequence, the protein is non-secreted and it functions in the cytoplasm. This reported result about cloning full-length cDNA of theanine synthetase gene would be beneficial for using biotechnology to improve tea plant cultivar resources and regulate L-theanine level.

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full-length cDNA cloning / sequence analysis / theanine synthetase

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LI Juan, DENG Ting-ting, WU Yang, LIU Shuo-qian, LI Qin, LIU Zhong-hua, HUANG Jian-an. Full-length cDNA Cloning and Sequence Analysis of Theanine Synthetase Gene in Camellia Sinensis[J]. Journal of Tea Science. 2011, 31(5): 411-418 https://doi.org/10.13305/j.cnki.jts.2011.05.012

References

[1] 张莹, 施兆鹏, 施玲. 茶氨酸的研究进展[J]. 天然产物研究与开发, 2003, 15(4): 369-372.
[2] Arai M, Matsuura T, Kakuda T.Variation of the yields and the chemical composition in leaves on nutriculture of tea plant[J]. Nippon Dojo Hiryogaku Zasshi, 1989, 60: 157-159.
[3] 汤皴. 茶氨酸的合成药理及其在食品中的应用[J]. 茶叶通报, 2002, 24(4): 19-21.
[4] 陈宗懋. 茶氨酸具有降压功能[J]. 中国茶叶, 1997, 19(2): 27.
[5] 丁王, 丁雪. 茶氨酸的抗疲劳作用研究[J]. 中国公共卫生, 2002, 18(3): 315-317.
[6] Sasaoka K, Kito M, Y O, et al. Some properties of the theanine synthesizing enzyme in tea seedlings[J]. Bio Chem, 1965, 29(11): 984-988.
[7] 李娟, 刘硕谦, 刘仲华, 等. 一种改良的茶树高质量RNA快速提取方法[J]. 福建茶叶, 2007(4): 34-35.
[8] 董志敏, 李英慧, 张宝石, 等. 一种获得大片段克隆的SMART全长cDNA文库构建方法[J]. 大豆科学, 2007, 12(3): 223-226.
[9] 王朝霞, 李叶云, 江昌俊, 等. 茶树巯基蛋白酶抑制剂基因的cDNA克隆与序列分析[J]. 茶叶科学, 2005, 25(3): l77-182.
[10] Mizutani M, Nakanishi H, Ema J, et al. Cloning of β-primeverosidase from tea Leaves, a key enzyme in tea aroma formation[J]. Plant Physiology, 2002, 130: 2164-2176.
[11] 邓婷婷. 安吉白茶白期叶与绿期叶差异表达基因的全长cDNA克隆与原核表达[D]. 长沙: 湖南农业大学, 2009.
[12] Ye Z, Connor JR. cDNA cloning by amplification of circularized first strand cDNAs reveals non-IRE-regulated iron-responsive mRNAs[J]. Biochem Biophys Res Commun, 2000, 275(1): 223-227.
[13] 董志敏, 李英慧, 张宝石, 等. 一种获得大片段克隆的SMART全长cDNA文库构建方法[J]. 大豆科学, 2007, 12(3): 223-226.