Molecular Cloning and Sequence Analysis of Chalcone Isomerase Gene of Tea Plant (Camellia sinensis)

马春雷，赵丽萍，张亚丽，陈亮

doi:10.13305/j.cnki.jts.2007.02.006

PDF(382 KB)

Journal of Tea Science ›› 2007, Vol. 27 ›› Issue (2) : 127-132. DOI: 10.13305/j.cnki.jts.2007.02.006

Molecular Cloning and Sequence Analysis of Chalcone Isomerase Gene of Tea Plant (Camellia sinensis)

MA Chun-lei^1,2, ZHAO Li-ping¹, ZHANG Ya-li^1,2, CHEN Liang^1,*

Author information +

History +

Abstract

Tea is one of the most popular non-alcoholic healthy beverages in the world, which possesses great value as a source of secondary metabolic products, such as catechins. Isolation and cloning of important functional genes of tea plant (Camellia sinensis) is of crucial significance for using biotechnology method to regulate the metabolism of tea plant. In this paper, the chalcone isomerase gene, which was an important functional gene of catechins biosynthesis pathway, was cloned from tea plant by using EST sequencing and RACE (rapid amplification of cDNA ends) approaches. The full-length cDNA of chalcone isomerase gene is 1 163 bp (GenBank Accession No. DQ904329), containing a 723bp open reading frame (ORF) encoding a 240 amino acid protein, and its 3′ untranslated region has an obvious polyadenylation signal. The deduced protein molecular weight was 26.4 kD and its theoretical isoelectric point was 5.19. Sequence analysis result showed that it is closely related with that of Lycopersicon esculentum.

Key words

chalcone isomerase / gene cloning / sequence analysis / tea plant (Camellia sinensis)

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

MA Chun-lei, ZHAO Li-ping, ZHANG Ya-li, CHEN Liang. Molecular Cloning and Sequence Analysis of Chalcone Isomerase Gene of Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2007, 27(2): 127-132 https://doi.org/10.13305/j.cnki.jts.2007.02.006

References

[1] 王锡洪, 奚彪. 略谈茶叶抗癌作用的研究与应用[J]. 中国茶叶, 2003(3): 20~21.
[2] Jane VH, Balz F. Tea catechins and polyphenols: health effects, metabolism, and antioxidant functions[J]. Food Science and Nutrition, 2003, 43: 89~143.
[3] Muir SR, Collins GJ, Robinson S, et al. Over expression of petunia chalcone isomerase in tomatoresults in fruits containing increased levels of flavonols[J]. Nature Biotechnology, 2001, 19: 470~474.
[4] Punyasiri PA, Abeysinghe IS, Kumar V, et al. Flavonoid biosynthesis in the tea plant Camellia sinensis: Properties of enzymes of the prominent epicatechin and catechin pathway[J]. Archives of Biochemistry and Biophysics, 2004, 431: 22~30.
[5] Furukawa T, Eshima A, Kouya M, et al. Coordinate expression of genes involved in catechin biosynthesis in Polygonum hydropiper cells[J]. The Plant Cell, 2002, 21: 385~389.
[6] 马春雷, 陈亮. 茶树功能基因分离克隆研究进展[J]. 分子植物育种, 2006, 4(3S): 16~22.
[7] 赵丽萍, 高其康, 陈亮, 等. 茶树基因芯片的研制和初步应用[J]. 茶叶科学, 2006, 26(3): 166~170.
[8] Chen L, Zhao LP, Gao QK. Generation and analysis of expressed sequence tags from the tender shoot cDNA library of tea plant (Camellia sinensis)[J]. Plant Science, 2005, 168: 359~363.
[9] Mamati EG, Liang YR. Genetic control studies to exploit flavonoids synthesis pathway in tea biochemical[J]. Journal of Tea Science, 2005, 25(2): 81~89.
[10] Tunen AJV, Koes RE, Spelt CE, et al. Cloning of the two chalcone flavanone isomerase genes from Petunia hybrida: coordinate, light-regulated and differential expression of flavonoid genes[J]. The EMBO Journal, 1988, 7(5): 1257~1263.
[11] Krol AVD, Lenting PE, Vennstra J, et al. Antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation[J]. Nature, 1988, 333: 866~869.
[12] Krol AVD, Mur LA, Beld M, et al. Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression[J]. The Plant Cell, 1990, 2: 291~299.
[13] Aida R, Yoshida K, Kondo T, et al. Copigmentation gives bluer flowers on transgenic torenia plants with the anti-sense dihydroflavonol1-4-reductase gene[J]. Plant Science, 2000, 160: 49~56.
[14] Zuker A, Tzfira T, Hagit BM, et al. Modification of flower color and fragrance by antisense suppression of the flavanone 3- hydroxylase gene[J]. Molecular Breeding, 2002, 9: 33~41.
[15] Verhoeyen ME, Bovy A, Collins G, et al. Increasing antioxidant levels in tomatoes through modification of the flavonoid biosynthetic pathway[J]. Journal of Experimental Botany, 2002, 53: 2099~2106.