茶树CsCDF1基因克隆及表达分析

doi:10.13305/j.cnki.jts.2015.05.015

Abstract

Abstract: The full-length cDNA of the first Dof gene (CsCDF1) was cloned from tea plant [Camellia sinensis (L.) O. Kuntze] by SMART-RACE cloning technology, and the bioinformatic analysis of the cloned gene were conducted by using online service. The expression profile of this gene in various tissues and with diurnal rhythm as well as in response to different dosage nitrogen treatment were investigated by using real-time fluorescent quantitative RT- PCR. The obtained cDNA sequence was 1β606 bp and contained a complete open reading frame encoding 464 amino acid residues with highly conserved DOF domain. The molecular weight and theoretical isoelectric point (PI) is 50.8βkDa and 5.52 respectively. The analysis by utilizing the BLAST software showed that the derived protein sequences shared separately 69%, 67% and 68% similarity with CDF (Cycling dof factor) proteins in Nicotiana to-mentosiformis, Solanum tuberosum and Nicotiana sylvestris. Phylogenetic analysis showed that the protein encoded by this gene was clustered into the same clade with Arabidopsis thaliana CDF, so it was named as CsCDF1. The expression of CsCDF1 dominated in roots rather than in one bud and two leaves or mature leaves in three phenotypes. During one day, CsCDF1 gene changed with the diurnal rhythm, and resupplying nitrogen after starvation for 2 weeks, the expression in response to different concentration of nitrogen was all raised in different tissues.

Key words: tea plant (Camellia sinensis), Dof gene, CsCDF1 gene, light regulation, nitrogen, expression analysis

CLC Number:

S571.1
Q52

HU Juan, WANG Liyuan, WEI Kang, CHENG Hao, ZHANG Chengcai, ZHANG Fen, WU Liyun. Cloning and Expression Analysis of CsCDF1 (Cycling Dof Factor 1) Gene in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2015, 35(5): 501-511.

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Cloning and Expression Analysis of CsCDF1 (Cycling Dof Factor 1) Gene in Tea Plant (Camellia sinensis)

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