Comparative Study on the Physicochemical Characteristics and Antioxidant Activities of Polysaccharides in Different Tea Cultivars

CHEN Xue; ZUO Xinxin; XU An'an; XU Ping; WANG Yuefei

doi:10.13305/j.cnki.jts.2022.06.011

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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (6) : 806-818. DOI: 10.13305/j.cnki.jts.2022.06.011

Comparative Study on the Physicochemical Characteristics and Antioxidant Activities of Polysaccharides in Different Tea Cultivars

CHEN Xue, ZUO Xinxin, XU An'an, XU Ping^*, WANG Yuefei

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Abstract

In the present work, 10 representative tea cultivars (Huangjingui, Tieguanyin, Qimenzhong, Liubaocha, Dahongpao, Zhuyeqi, Longjing 43, Baiyeyihao, Zhonghuangyihao and Fudingdabai) in the same tea garden were selected and their fresh leaves were dried directly by microwave. Tea polysaccharides (TPS) from the 10 cultivars were prepared by water extraction. Their physical and chemical characteristics were compared and analyzed by chemical analysis, high performance liquid chromatography (HPLC), infrared spectroscopy (IR), gel chromatography, scanning electron microscope (SEM), particle size measurement and Zeta potential analysis. And their antioxidant abilities including DPPH radical scavenging ability, ABTS radical scavenging ability and ferric ion reducing ability were determined as well. It was found that TPS was a kind of water-soluble acid glycoprotein, which mainly consisted of uronic acid, neutral sugar, protein and polyphenols. Meanwhile, the monosaccharide components of the 10 TPSs were mainly composed of galacturonic acid, glucose, galactose and arabinose. Principal component analysis (PCA) shows that the basic composition of TPS in Baiyeyihao and Longjing 43 were significantly different from other tea cultivars. Furthermore, the antioxidant activity of TPS in Baiyeyihao was the strongest among the obtained TPS. Correlation analysis indicates that the antioxidant capacity of TPS might be related to the contents of polyphenols and proteins.

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antioxidant / physicochemical characteristics / polysaccharides / tea cultivar

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CHEN Xue, ZUO Xinxin, XU An'an, XU Ping, WANG Yuefei. Comparative Study on the Physicochemical Characteristics and Antioxidant Activities of Polysaccharides in Different Tea Cultivars[J]. Journal of Tea Science. 2022, 42(6): 806-818 https://doi.org/10.13305/j.cnki.jts.2022.06.011

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