Effects of Exogenous 24-epibrassinolide on the Photosynthetic Characteristics of Tea Plants (Camellia sinensis)

LI Zhixin; LI Xin; FAN Lichao; HAN Wenyan

doi:10.13305/j.cnki.jts.2015.06.005

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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (6) : 543-550. DOI: 10.13305/j.cnki.jts.2015.06.005

Effects of Exogenous 24-epibrassinolide on the Photosynthetic Characteristics of Tea Plants (Camellia sinensis)

LI Zhixin^1,2, LI Xin^1,*, FAN Lichao^1,2, HAN Wenyan^1,*

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Abstract

24-epibrassinolide (EBR) is an important plant hormone, which plays an important role in a variety of adverse resistance in plants. The effects of exogenous EBR on photosynthesis, Rubisco and FBPase activity and the expression of related-gene from tea leaves (Camellia sinensis cv. ‘Longjing 43’, ‘Xiangguliaobaihao’, and ‘Qingmingzao’) under field condition were investigated. It was showed that after exogenous EBR (0.1βmg·L^-1) treatment: Net photosynthetic rate of these three cultivars increased by 49.06%, 45.49% and 92.34% respectively. Vc_max increased by 21.82%, 21.68 % and 21.68%, and J_max increased by 17.16%, 23.86% and 23.86%, respectively. The activity of Rubisco and FBPase increased significantly. Furthermore, the expression of Rubisco and FBPase related-gene increased significantly. These results suggested that application of exogenous EBR could enhance Vc_max, J_max and the activity of Rubisco and FBPase effectively, which ultimately promoted the photosynthetic carbon fixation, and the photosynthetic rate of tea plants.

Key words

24-epibrassinolide / gene expression / photosynthetic characteristics / tea plant (Camellia sinensis)

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LI Zhixin, LI Xin, FAN Lichao, HAN Wenyan. Effects of Exogenous 24-epibrassinolide on the Photosynthetic Characteristics of Tea Plants (Camellia sinensis)[J]. Journal of Tea Science. 2015, 35(6): 543-550 https://doi.org/10.13305/j.cnki.jts.2015.06.005

References

[1] 陶汉之, 王镇恒. 我国茶树光合作用研究进展及发展趋势[J]. 茶叶科学, 1995, 15(1): 1-8.
[2] 岳川, 曾建明, 章志芳, 等. 茶树中植物激素研究进展[J]. 茶叶科学, 2012, 32(5): 382-392.
[3] 周宇飞, 王德权, 陆樟镳, 等. 干旱胁迫对持绿性高粱光合特性和内源激素ABA、CTK含量的影响[J]. 中国农业科学, 2014(4): 655-663.
[4] 陈瑛, 陶文沂. 几种激素对茶愈伤组织合成茶氨酸的影响[J]. 无锡轻工大学学报, 1998(1): 76-79.
[5] 陶汉之, 陶迁, 程茱萸, 等. 外源激素和微量元素对茶籽萌发过程中酶活性影响的研究[J]. 作物学报, 1999(6): 712-717.
[6] 柴红玲, 郑生宏, 叶志森. 外源激素对中茶108扦插育苗的影响[J]. 浙江农业科学, 2015(2): 183-186.
[7] 郑红发, 黄亚辉, 粟本文, 等. 施用外源激素对茶叶安全性影响的研究[J]. 茶叶通讯, 2005(2): 4-6.
[8] Grove M D, Spencer F G, Rohwededer W K, et al. Brassinolide, a plant growth promoting steroid isolated from Brassicanapus pollen[J]. Nature, 1979, 281(2): 216-217.
[9] Santner A, Li C V, Estelle M.Plant hormones are versatile chemical regulators of plant growth[J]. Nature Chemical Biology, 2009, 5(5): 301-307.
[10] Vardhini B V, Rao S R.Effect of brassinosteroids on growth,metabolite content and yield of Arachis hypogaea[J]. Phytochemistry, 1998, 48(6): 927-930.
[11] Braun P, Wild A.The influence of brassinosteroid on growth and parameters of photosynthesis of wheat and mustard plants[J]. Journal of Plant Physiology, 1984, 116(2): 189-196.
[12] Saygideger S, Deniz F.Effect of 24-epibrassinolide on biomass, growth and free proline concentration in Spirulina platensis (Cyanophyta) under NaCl stress[J]. Plant Growth Regulation, 2008, 56(3): 219-223.
[13] Yuan G F, Jia C G, Li Z, et al. Effect of brassinosteroids on drought resistance and abscisic acid concentration in tomato under water stress[J]. Scientia Horticulturae, 2010, 126(2): 103-108.
[14] Bajguz A, Hayat S.Effects of brassinosteroids on the plant responses to environmental stresses[J]. Plant Physiology and Biochemistry, 2009, 47(1): 1-8.
[15] 洪海林. 喷施BR-120对春茶有明显的增产效应[J]. 中国茶叶, 1995, 15(4): 25.
[16] McMurtrie R E, Wang Y P. Mathematical models of the photosynthetic response of tree stands to rising CO₂ concentrations and temperatures[J]. Plant Cell and Environment, 1993, 16(1): 1-13.
[17] Du Y C, Nose A, Kawamitsu Y, et al. An improved spectrophotometric determination of the activity of ribulose 1,5-bisphosphate carboxylase[J]. Japanese Journal of Crop Science, 1996, 65(4): 714-721.
[18] Nakano H, Muramatsu S, Makino A, et al. Relationship between the suppression of photosynthesis and starch accumulation in the pod-removed bean[J]. Australian Journal of Plant Physiology, 2000, 27(2): 167-173.
[19] Scheibe R, Fickenscher K, Ashton A R.Studies on the mechanism of the reductive activation of NADP-malate dehydrogenase by thioredoxin m and low molecular weight thiols[J]. Biochimica et Biophysica Acta, 1986, 870: 191-197.
[20] Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method[J]. Methods, 2001, 25(4): 402-408.
[21] Farquhar G D, Sharkey T D.Stomatal conductance and photosynthesis[J]. Annual Review of Plant Physiology, 1982: 33317-33345.
[22] Farquhar G D, Caemmerer S V, Berry J A.A biochemical model of photosynthetic CO₂ assimilation in leaves of C3 species[J]. Planta, 1980, 149(1): 78-90.
[23] Portis A R.Rubisco activase - Rubisco's catalytic chaperone[J]. Photosynthesis Research, 2003, 75(1): 11-27.
[24] Stitt M, Cseke C, Buchana B B.Regulation of fructose-2,6-bisphosphate concentration in spinach leaves[J]. Eur J Biochem, 1984, 143(1): 89-93.
[25] 胡文海, 黄黎锋, 毛伟华, 等. 油菜素内酯对黄瓜苗期叶片光合机构调节作用的研究[J]. 园艺学报, 2006(4): 762-766.
[26] 李宁, 郭世荣, 束胜, 等. 外源24-表油菜素内酯对弱光胁迫下番茄幼苗叶片形态及光合特性的影响[J]. 应用生态学报, 2015(3): 847-852.
[27] 彭予咸, 胡留杰, 邓敏, 等. 植物生长调节剂对茶新梢叶绿素荧光特性的影响[J]. 西南农业学报, 2013(2): 514-519.
[28] Whitney S M, Houtz R L, Alonso H.Advancing our Understanding and capacity to engineer nature's CO₂-sequestering enzyme, Rubisco[J]. Plant Physiology, 2011, 155(1): 27-35.
[29] Andersson I,Backlund A.Structure and function of Rubisco[J]. Plant Physiology and Biochemistry,2008: 46275-46291.
[30] Jiang D A, Xu Y F.Internal dominant factors for declination of photosynthesis during rice leaf senescence[J]. Journal of Zhejiang Agricultural University, 1995, 21(5): 533-538.
[31] Wang J Y, Zhu S G, Xu C F.Biochemistry [M]. Beijing: Higher Education Press, 2002.
[32] Long S P, Bernacchi C J.Gas exchange measurements, what can they tell us about the underlying limitations to photosynthesis? Procedures and sources of error[J]. Journal of Experimental Botany, 2003, 54(392): 2393-2401.
[33] Stitt M.Fructose-2,6-bisphosphate as a regulatory molecule in plants[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1990, 41153-41185.
[34] 张彦敏, 周广胜. 植物叶片最大羧化速率及其对环境因子响应的研究进展[J]. 生态学报, 2012, 32(18): 5907-5917.