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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (2): 183-192.doi: 10.13305/j.cnki.jts.2018.02.009

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Cloning and Expression Analysis of CsSnRK2.1 and CsSnRK2.2 Genes in Tea Plant (Camellia sinensis) under Abiotic Stress

ZHANG Yongheng1, WANG Siqing1, CHEN Jiangfei1, WANG Weidong1, ZHOU Tianshan1, XIAO Bin1, YANG Yajun1,2,*, YU Youben1,*   

  1. 1. College of Horticulture, Northwest A&F University, Yangling 712100, China;
    2. Tea Research Institute of Chinese Academy, Agricultural Sciences/National Center for Tea Improvement / Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China
  • Received:2017-06-05 Revised:2017-08-01 Online:2018-04-15 Published:2019-08-28

Abstract: Sucrose non-fermenting-1-related protein kinase (SnRK) is a kind of serine/threonine protein kinases widely exist in plants. Among them, members of SnRK2 family play a vital role in plant response to stresses. In order to investigate the molecular mechanisms of Camellia sinensis SnRK2 in response to abiotic stresses, two SnRK2 genes from tea plant were cloned and named as CsSnRK2.1 (GenBank accession code: MG026837) and CsSnRK2.2 (GenBank accession code: MF662805) respectively. Bioinformatics analysis showed that they contained 358 and 337 amino acids respectively, which harbored a conserved ATP binding site and Ser/Thr kinase domain and were highly similar to SnRK2 protein kinase of Arabidopsis thaliana and maize. The expression of CsSnRK2.1 was transiently induced and then decreased by high salinity (100βmmol·L-1 NaCl), drought (20% PEG6000) and ABA(100βμmol·L-1) stress, but showed no significant changes under low (4℃) or high temperature treatments (38℃). By contrast, CsSnRK2.2 was strongly induced by high salt and temperature treatments. The results revealed that CsSnRK2.1 and CsSnRK2.2 might be closely related to stress responses in tea plant.

Key words: tea plant (Camellia sinensis), SnRK2s, cloning, abiotic stress

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