The water extract of pu-erh tea was extracted with chloroform, ethyl acetate, n-butanol in turn. The remaining water pigment-theabrownin obtained was installed in hydroxypropyl glucan gel (sephadex LH-20) column with acetone-aqueous solution for elution and the fractions of theabrownin were collected respectively and their physico-chemical and spectral properties were studied preliminarily. The results indicated that the 40% acetone-aqueous solution was the optimum eluent and flow was 0.05BV/h and classification of theabrownin was best in presenting 6 obvious strips in column, with difference of their chromatism parameter values, total reducing power, pH value, conventional component, UV and IR spectroscopy scanning, and stability etc. The study further demonstrated complexity of compositions and properties of theabrownin. At the same time, this investigation laid a foundation for the further research of bioactivities and chemical essence of theabrownin.
YANG Xin-he
,
WANG Li-li
,
HUANG Jian-an
,
WU Wen-liang
,
LIU Zhong-hua
. Preliminary Study on Fractions and Correlative Properties of Theabrownin from Pu-erh Tea[J]. Journal of Tea Science, 2011
, 31(3)
: 187
-194
.
DOI: 10.13305/j.cnki.jts.2011.03.006
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