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Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (3): 187-194.doi: 10.13305/j.cnki.jts.2011.03.006

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Preliminary Study on Fractions and Correlative Properties of Theabrownin from Pu-erh Tea

YANG Xin-he1,2, WANG Li-li1,3, HUANG Jian-an1,3, WU Wen-liang1,3, LIU Zhong-hua1,3*   

  1. 1. Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China;
    2. School of Life Science and Technology, Xiaogan College, Xiaogan 432000, China;
    3. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingedients, Changsha 410128, China;
  • Received:2010-12-13 Revised:2011-02-20 Online:2011-06-15 Published:2019-09-06

Abstract: The water extract of pu-erh tea was extracted with chloroform, ethyl acetate, n-butanol in turn. The remaining water pigment-theabrownin obtained was installed in hydroxypropyl glucan gel (sephadex LH-20) column with acetone-aqueous solution for elution and the fractions of theabrownin were collected respectively and their physico-chemical and spectral properties were studied preliminarily. The results indicated that the 40% acetone-aqueous solution was the optimum eluent and flow was 0.05BV/h and classification of theabrownin was best in presenting 6 obvious strips in column, with difference of their chromatism parameter values, total reducing power, pH value, conventional component, UV and IR spectroscopy scanning, and stability etc. The study further demonstrated complexity of compositions and properties of theabrownin. At the same time, this investigation laid a foundation for the further research of bioactivities and chemical essence of theabrownin.

Key words: pu-erh tea, theabrownin, fraction, property

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