普洱茶茶褐素对大鼠尿液影响的代谢组学研究

doi:10.13305/j.cnki.jts.2016.05.007

Abstract

Abstract: The study was aimed to understand the effects of Pu-erh theabrownin (TB) (Mw>50βkDa) on the metabolism of rat urine by nuclear magnetic resonance (NMR)-based metabolomics. Rats were divided into four groups including rats receiving a basic diet alone (control group), a basic diet and intragastric infusion of Pu-erh TBs (TB infusion group), a high-lipid diet alone (high lipid group) and high-lipid diet and intragastric infusion of Pu-erh TBs (high lipid-TB infusion group) for 14 d. Urine samples were analyzed by biochemical assays, NMR and orthogonal partial least-square-discriminant analysis (OPLS-DA) to identify differential metabolism among these groups. The results showed that considerable differences in metabolite profiles of rat urine were detected between control group and TB infusion group, and between high lipid group and high lipid-TB infusion group. Valine, citric acid, taurine, propionate, α-ketoglutaric acid and β-hydroxybutyrate were identified as metabolic markers of Pu-erh TB. Besides, Pu-erh TB was likely to influence several metabolic pathways in rat, including amino acid metabolism, energy metabolism, tricarboxylic acid cycle, lipid metabolism and oxidative stress.

Key words: theabrownins, metabolomics, NMR

CLC Number:

LIU Jian, PENG Chunxiu, SHENG Jun, GAO Bin, GONG Jiashun. Metabolomic Analysis of Rat Urine after Intragastric Infusion of Pu-erh Theabrownins[J]. Journal of Tea Science, 2016, 36(5): 491-499.

References 37

[1]	龚加顺, 周红杰, 张新富, 等. 云南晒青绿毛茶的微生物固态发酵及成分变化研究[J]. 茶叶科学, 2005, 25(4): 300-306.
[2]	Hou Y, Shao WF, Xiao R, et al.Pu-erh tea aqueous extracts lower atherosclerotic risk factors in a rat hyperlipidemia model[J]. Experimental Gerontology, 2009, 44(6/7): 434-439.
[3]	Gong JS, Peng CX, Chen T, et al.Effects of theabrownin from Pu-erh Tea on the metabolism of serum lipids in rats: Mechanism of action[J]. Journal of Food Science, 2010, 75(6): 182-189.
[4]	Yan SJ, Wang L, Li Z, et al.Inhibition of advanced glycation end product formation by Pu-erh tea ameliorates progression of experimental diabetic nephropathy[J]. Journal of Agricultural and Food Chemistry, 2012, 60(16): 4102-4110.
[5]	秦谊, 龚加顺, 张惠芬, 等. 普洱茶茶褐素提取工艺及理化性的初步研究[J]. 林产化学与工业, 2009, 29: 95-98.
[6]	龚加顺, 陈文品, 周红杰, 等. 云南普洱茶特征成分的功能与毒理学评价[J]. 茶叶科学, 2007, 27(3): 201-2l0.
[7]	Wang QP, Peng CX, Gong JS.Effects of enzymatic action on the formation of theabrownin during solid state fermentation of Pu-erh tea[J]. J Sci Food Agric, 2011, 13(91): 2412-2418.
[8]	周红杰, 秘鸣, 韩俊. 普洱茶的功效及品质形成机理研究进展[J]. 茶叶科学, 2003, 23(2): 75-77.
[9]	郭韦韦. 普洱茶减肥功效评价与研究[D]. 昆明: 昆明医学院, 2011: 29-38.
[10]	熊昌云. 普洱茶降脂减肥功效及作用机理研究[D]. 杭州: 浙江大学, 2012: 25-93.
[11]	陈婷. 普洱茶茶褐素降血脂功效及其作用机制研究[D]. 云南: 云南农业大学, 2009: 25-50.
[12]	王秋萍, 龚加顺. “紫娟” 普洱茶茶褐素对高脂饮食大鼠生长发育的影响[J]. 茶叶科学, 2012, 32(1): 87-94.
[13]	高斌, 彭春秀, 龚加顺, 等. 普洱茶茶褐素对大鼠激素敏感性脂肪酶活性及其mRNA表达的影响[J]. 营养学报, 2010, 4(32): 362-366.
[14]	Nicholson JK, Connelly J, Lindon JC, et al .Metabonomics: a platform for studying drug toxicity and gene function[J]. Nat Rev Drug Discov, 2002, 1(2): 153-161.
[15]	Azmi J, Griffin JL, Shore RF, et al.Chemometric analysis of biofluids following toxicant induced hepatotoxicity: a metabonomic approach to distinguish the effects of 1-naphthylisothiocyanate from its products[J]. Xenobiotica, 2005, 35(8): 839-852.
[16]	Griffin JL.Metabonomics: NMR spectroscopy and pattern recognition analysis of body fluids and tissues for characterization of xenobiotic toxicity and disease diagnosis[J]. Current Opinion in Chemical Biology, 2003, 7(5): 648-654.
[17]	杨大鹏, 史文斌, 陈一江, 等. 不同微生物发酵的云南普洱茶样中茶褐素提取物的化学成分分析[J]. 林产化学与工业, 2010, 30(1): 49-52.
[18]	敬明武, 葛宇杰, 刘科亮. 茶色素胶囊对大鼠辅助调节血脂的研究[J]. 中国现代医生, 2007, 45(5): 18-19.
[19]	中华人民共和国卫生部. 保健食品检验与评价技术规范[M]. 北京: 人民卫生出版, 2003: 2.
[20]	刘树民, 崔立然. 代谢组学技术在中药毒性研究中的应用前景[J]. 毒理学杂志, 2008, 22(2): 155.
[21]	Wilson ID, Nicholson JK.Solid phase extraction chromatography and NMR spectroscopy (SPEC-NMR) for the rapid identification of drug metabolites in urine[J]. J Pharm Biomed, 1988, 6(2): 151-165.
[22]	Gartland KPR, Eason CT, Wade KE.Proton NMR spectroscopy of bile for onitoring the excretion of endogenous and xenobiotic metabolites: application to paraminophenol[J]. J PharmBiomed,1989, 7(6): 699-707.
[23]	Tuli PK, Nicholson JK, Timbrell JAA.Study of the metabolism of dimethylformamide in the rat by high resolution proton NMR spectroscopy[J]. J Pharm Biomed, 1989, 7(4): 499-505.
[24]	Wilson ID, Wade KE, Nicholson JK.Analysis of biological fluids by high-field nuclear magnetic resonance spectroscopy[J]. Trends Anal Chem, 1989, 8(10): 368-374.
[25]	Liang X, Chen X, Liang Q, et al.Metabonomic study of Chinese medicine Shuanglong formula as an effective treatment for myocardial infarction in rats[J]. J Proteome Res, 2011, 10(2): 790-799.
[26]	Sumner LW, Mendes P, Dixon RA.Plant metabolomics: largescale phytochem is try in the functional genomics era[J]. Phytochemistry, 2003, 62(6): 817-836.
[27]	Rochfort S.Metabolomics reviewed: a new “omics” platform technology for systems biology and implications for natural products research[J]. J Nat Prod, 2005, 68(12): 1813-1820.
[28]	Dettmer K, Aronov PA, Hammock BD.Mass spectrometry-based metabolomics[J]. Mass Spectrom Rev, 2007, 26(1): 51-78.
[29]	林金鸳, 许劲松. 菊粉的功能与应用[J]. 四川食品与发酵, 1999(4): 57-59.
[30]	Yoshikawa H, Tajiri Y, Sako Y, et al.Effects of free fatty acids on beta-cell functions: a possible involvement of peroxisome proliferator activated receptors alpha or pancreatic/duodenal homeobox[J]. Metabolism, 2001, 50(5): 613-618.
[31]	Stanko RT, Adibi SA.Inhibition of lipid accumulation and enhancement of energy expediture by the addition of pyruvate and dihydroxyacetone to a rat diet[J]. Metabolism, 1986, 35(2): 182-186.
[32]	Rahman MM, Park HM, Kim SJ, et al.Taurine prevent shypertension and increases exercise capacity in rats with fructose-induced hypertension[J]. Am J Hypertens, 2011, 24(5): 574-581.
[33]	Gavachan CL, Holmes E, Len ZE, et al.An NMR-based metabonomic approach to investigate the biochemical consequences of genetic strain differences;application to the C57BL10J and Alpk:Apfcd mouse[J]. FEBS Lett, 2000, 484(3): 169.
[34]	Rull A, Vinaixa M, Angel Rodriguez M, et al.Metabolic phenotyping of genetically modified mice: An NMR metabonomic approach[J]. Biochimie, 2009, 91(8): 1053-1057.
[35]	Thiess S, Becskei C, Tomsa K, et al.Effects of a high carbohydrate and high fat diet on plasma metabolite levels and on iv glucose tolerance test in intact and neutered male cats[J]. J Feline Med Surg, 2004, 4(6): 207-218.
[36]	Shimazu T, Hirschey MD, Newman J, et al.Suppression of oxidative stress by β-hydroxybutyrate, an endogenous histone deacetylase inhibitor[J]. Science, 2013, 339(11): 211-214.
[37]	Jiang L, Huang J, Wang Y, et al.Metabonomic Analysis Reveals the CCl4-Induced Systems Alterations for Multiple Rat Organs[J]. J Proteome Res, 2012, 11(7): 3848-3859.

Metabolomic Analysis of Rat Urine after Intragastric Infusion of Pu-erh Theabrownins

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 37

Related Articles 7

Metrics

Comments

Recommended 10

[1]	GAO Jianjian, CHEN Dan, PENG Jiakun, WU Wenliang, CAI Liangsui, CAI Yawei, TIAN Jun, WAN Yunlong, SUN Weijiang, HUANG Yan, WANG Zhe, LIN Zhi, DAI Weidong. Comparison on Chemical Components of Yunnan and Fuding White Tea Based on Metabolomics Approach [J]. Journal of Tea Science, 2022, 42(5): 623-637.
[2]	CHEN Jiajia, ZHU Chensong, ZHU Wenwei, SHANG Hu, LIN Lin, LUO Yuqin, SUN Weijiang. Analysis of the Metabolism of Amino Acids during the Withering of White Tea [J]. Journal of Tea Science, 2021, 41(4): 471-481.
[3]	YUE Wenjie, JIN Xinyi, CHEN Mingjie, YE Naixing, GUO Li, ZHAO Feng. Analysis of Metabolite Changes in the Natural Withering Process of Fu′an White Tea Based on Non-targeted Metabolomics Approach [J]. Journal of Tea Science, 2021, 41(3): 379-392.
[4]	YU Xinlei, AI Yujie, QU Fengfeng, AI Zeyi, LIU Shuyuan, CHEN Yuqiong, NI Dejiang. Metabolomics Application in the Study of Tea Quality Formation [J]. Journal of Tea Science, 2018, 38(1): 20-32.
[5]	YANG Chen, DAI Weidong, LYU Meiling, LI Pengliang, LIU Xu, TIAN Jun, WAN Yunlong, LI Ji, LIN Zhi. Study on the Chemical Constituents of Pu-erh Teas from Different Areas by UHPLC-Q-TOF/MS [J]. Journal of Tea Science, 2017, 37(6): 605-615.
[6]	LI Ran, CHEN Shan-shan, YU Jie, YANG Lei, WANG Hong-zhi, ZHANG Xue-long. Determination of Water Content of Tea by Low Field Nuclear Magnetic Resonance Technology [J]. Journal of Tea Science, 2010, 30(6): 440-444.
[7]	GAO Qing-qing, JIANG He-yuan, ZHANG Jian-yong, YUAN Xin-yue, KOU Xiao-hong, GAO Qi, LIU Xiao-hui, KE Chang-qiang. Separation, Purification and Structure Identification of Flavonol Glycosides in Black Tea [J]. Journal of Tea Science, 2008, 28(4): 277-281.