Metabolomics Application in the Study of Tea Quality Formation

YU Xinlei; AI Yujie; QU Fengfeng; AI Zeyi; LIU Shuyuan; CHEN Yuqiong; NI Dejiang

doi:10.13305/j.cnki.jts.2018.01.003

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Journal of Tea Science ›› 2018, Vol. 38 ›› Issue (1) : 20-32. DOI: 10.13305/j.cnki.jts.2018.01.003

Metabolomics Application in the Study of Tea Quality Formation

YU Xinlei, AI Yujie, QU Fengfeng, AI Zeyi, LIU Shuyuan, CHEN Yuqiong, NI Dejiang^*

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Abstract

Metabolomics, an important branch of systems biology, mainly study the changes of small molecule metabolites affected by the internal and external environments (gene or environmental changes). Metabolomics has been widely applied in the field of natural medicine development, botany, microbiology and food safety researches. The application of metabolomics in tea science has also been developed rapidly in recent years. In this article, the application of metabolomics in three major aspects of tea researches (growth and development, the quality formation during processing and the evaluation of tea function) was reviewed and discussed. We believed that metabolomics would play an irreplaceable role in the analysis of gene function, the regulation of metabolic network, the cultivation and the quality improvement in tea.

Key words

Camellia sinensis / growth and development / metabolomics / tea functions / tea processing

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YU Xinlei, AI Yujie, QU Fengfeng, AI Zeyi, LIU Shuyuan, CHEN Yuqiong, NI Dejiang. Metabolomics Application in the Study of Tea Quality Formation[J]. Journal of Tea Science. 2018, 38(1): 20-32 https://doi.org/10.13305/j.cnki.jts.2018.01.003

References

[1] Oliver F, Joachim K, Peter D, et al.Metabolite profiling for plant functional genomics[J]. Nature Biotechnology, 2000, 18(11): 1157-1161.
[2] Oliver F.Metabolomics—the link between genotypes and phenotypes[J]. Plant Molecular Biology, 2002(48): 155-171.
[3] 张丽, 姬厚伟, 黄锡娟, 等. 植物代谢组学及其在烟草上的应用进展[J]. 中国烟草学报, 2015, 21(5): 126-134.
[4] 尹恒, 李曙光, 白雪芳, 等. 植物代谢组学的研究方法及其应用[J]. 植物学通报, 2015, 22(5): 532-540.
[5] Josep R, Milena Z,Jana H.Advances in high-resolution mass spectrometry based on metabolomics studies for food—a review[J]. Food Additives & Contaminants: Part A, 2015, 32(10): 1685-1708.
[6] 漆小泉, 王玉兰, 陈晓亚. 植物代谢组学: 方法与应用[M]. 北京: 化学工业出版社, 2011: 9-11.
[7] Li C F, Yao M Z, Ma C L, et al.Differential metabolic profiles during the albescent stages of‘Anji Baicha’(Camellia sinensis)[J]. PLOS ONE, 2015(10): 1-18.
[8] Zhang Q F, Shi Y Z, Ma L F, et al.Metabolomic analysis using ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOFMS) uncovers the effects of light intensity and temperature under shading treatments on the metabolites in tea[J]. PLOS ONE, 2014, 9(11): 1-10.
[9] Maria D, Antiochia R, Anatoly P, et al.Untargeted and targeted methodologies in the study of tea[J]. Food Research International, 2014(63): 275-289.
[10] 杨亦扬, 张佳, 王川丕, 等. 茶树鲜叶品质成分浸提方法比较及应用[J]. 茶叶科学, 2014, 34(2): 137-143.
[11] 许国旺, 张磊, 曾仲大, 等. 基于气相色谱-质谱联用与液相色谱-质谱联用的非靶向代谢组学用于3类茶叶中化学成分分析[J]. 色谱, 2014, 32(8): 804-816.
[12] Lee J E, Lee B J, Chung J O, et al.1H NMR-based metabolomic characterization during green tea(Camellia sinensis) fermentation[J]. Food Research International, 2011(44): 597-604.
[13] Makoto K, Kozo O, Hiroshi K, et al.Determination of dimethylsulfide in the head space vapor of green tea by gas chromatography[J]. Agricultural and Biological Chemistry, 1977, 41(11): 2285-2287.
[14] 陈亚东, 曹玉廷, 干宁, 等. 分子印迹固相萃取和电喷雾质谱法联用测定茶叶中四种儿茶素[J]. 茶叶科学, 2009, 29(3): 231-235.
[15] Yang T, Zhu Y, Shao C Y, et al.Enantiomeric analysis of linalool in teas using headspace solid-phase micro extraction with chiral gas chromatography[J]. Industrial Crops and Products, 2016(83): 17-23.
[16] 张佳, 王川丕, 阮建云. GC-MS及GC测定茶叶中主要游离氨基酸的方法研究[J]. 茶叶科学, 2010, 30(6): 445-452.
[17] Wipawee P, Taksehi B, Tsutomu Y, et al.Quality prediction of Japanese green tea using pyrolyzer coupled GC/MS based metabolic fingerprinting[J]. Journal of Agricultural and Food Chemistry, 2008, 56: 744-750.
[18] Christian S, Peter S.Characterization of the key aroma compounds in the beverage prepared from Darjeeling black tea quantitative differences between tea leaves and infusion[J]. Journal of Agricultural and Food Chemistry, 2006, 54: 916-924.
[19] Dong F, Yang Z Y, Susanne B, et al.Herbivore-induced volatiles from tea (Camellia sinensis) plants and their involvement in intraplant communication and changes in endogenous nonvolatile metabolites[J]. Journal of Agricultural and Food Chemistry, 2011, 59: 13131-13135.
[20] Viola S Y L, Dou J P, Ronald J Y C, et al. Massive accumulation of gallic acid and unique occurrence of myricetin, quercetin, and kaempferol in preparing old Oolong tea[J]. Journal of Agricultural and Food Chemistry, 2008, 56(17): 7950-7956.
[21] Alexandr Y Y, Boris V N, Emilie C, et al.Determination of the chemical composition of tea by chromatographic methods—a review[J]. Journal of Food Research, 2015, 4(3): 56-87.
[22] 张正竹, 宛晓春, 陶冠军. 茶鲜叶中糖苷类香气前体的液质联用分析[J]. 茶叶科学, 2005, 25(4): 275-281.
[23] Hitoshi I, Toshiyuki W, Yukiko K, et al.Quantitation of chafurosides A and B in tea leaves and isolation of prechafurosides A and B from Oolong tea leaves[J]. Journal of Agricultural and Food Chemistry, 2009, 57(15): 6779-6786.
[24] 赵燕, 丁立建. 核磁共振技术在植物代谢研究中的应用[J]. 现代仪器与医疗, 2013, 19(1): 21-24.
[25] Justin J J.H Moktar A, Fatma Y U?, et al. Structural annotation and elucidation of conjugated phenolic compounds in black, green, and white tea extracts[J]. Journal of Agricultural and Food Chemistry, 2012, 60: 8841-8850.
[26] Yue Y, Chu G X, Liu X S, et al.TMDB: a literature-curated database for small molecular compounds found from tea[J]. BMC Plant Biology, 2014, 14:243. DOI: 10.1186/s12870-014-0243-1.
[27] Liu J W, Zhang Q F, Liu M Y, et al.Metabolomic analyses reveal distinct change of metabolites and quality of green tea during the short duration of a single spring season[J]. Journal of Agricultural and Food Chemistry, 2016, 64: 3302-3309.
[28] Chao L, Cheng S, Zhang Y, et al.Transcriptomic, proteomic and metabolic changes in Arabidopsis thaliana leaves after the onset of illumination[J]. BMC Plant Biology, 2016(16): 43. DOI 10.1186/s12870-016-0726-3.
[29] Wen W W, Li D, Li X, et al.Metabolome-based genome-wide association study of maize kernel leads to novel biochemical metabolome-based genome-wide association study of maize kernel leads to novel biochemical insights[J]. Nature Communications, 2014(2): 1-10.
[30] Chen W, Gao Y Q, Xie W B, et al.Genome-wide association analyses provide genetic and biochemical insights into natural variation in rice metabolism[J]. Nature Genetics, 2014, 46(7): 714-720.
[31] Tai Y, Wei C, Yang H, et al.Transcriptomic and phytochemical analysis of the biosynthesis of characteristic constituents in tea(Camellia sinensis) compared with oil tea, 2015(15): 190. DOI: 10.1186/s12870-015-0574-6.
[32] Xia J G, Igor V S, Beomsoo H, et al.MetaboAnalyst 3.0-making metabolomics more meaningful[J]. Nucleic Acids Research, 2015(4): 1-7.
[33] Lee J E, Lee B J, Chung J O, et al.Geographical and climatic dependencies of green tea (Camellia sinensis) metabolites: A ¹H NMR-based metabolomics study[J]. Journal of Agricultural and Food Chemistry, 2010, 58: 10582-10589.
[34] Lee J E, Lee B J, Hwang J, et al.Metabolic dependence of green tea on plucking positions revisited a metabolomic study[J]. Journal of Agricultural and Food Chemistry, 2011, 59: 10579-10585.
[35] Elis D P, Ieda S S, Romà T.Analytical investigation of secondary metabolites extracted from Camellia sinensis L. leaves using a HPLC-DAD-ESIMS data fusion strategy and chemometric methods[J]. Journal of Chemometrics, 2016, 30: 75-85.
[36] Dai W D, Qi D D, Yang T, et al.Nontargeted analysis using ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry uncovers the effects of harvest season on the metabolites and taste quality of tea (Camellia sinensis L.)[J]. Journal of Agricultural and Food Chemistry, 2015, 63: 9869-9878.
[37] Too C J, Wanyoko K J, Kinyanjui T, et al.Effect of seasons on theanine levels in different Kenyan commercially released tea cultivars and its variation in different parts of the tea shoot[J]. Food and Nutrition Sciences, 2015, 6: 1450-1459.
[38] Feng L, Gao M J, Hou R Y, et al.Determination of quality constituents in the young leaves of albino tea cultivars[J]. Food Chemistry, 2014(155): 98-104.
[39] 杨亦扬, 马立锋, 黎星辉, 等. 氮素水平对茶树新梢叶片代谢谱及其昼夜变化的影响[J]. 茶叶科学, 2013, 33(6): 491-499.
[40] Xu Q S, Wang Y, Ding Z T, et al.Aluminum induced metabolic responses in two tea cultivars[J]. Plant Physiology and Biochemistry, 2016(101): 162-172.
[41] Kang M K, Jung N C, Jiyoung K, et al.Metabolomics analysis reveals the compositional differences of shade grown tea (Camellia sinensis L.)[J]. Journal of Agricultural and Food Chemistry, 2010, 58: 418-426.
[42] Lee L S, Choi J H, Son N, et al.Metabolomic analysis of the effect of shade treatment on the nutritional and sensory qualities of green tea[J]. Journal of Agricultural and Food Chemistry, 2013(1): 113-121.
[43] Yang Z Y, Eiji K, Tsuyoshi K, et al.Characterisation of volatile and non-volatile metabolites in etiolated leaves of tea(Camellia sinensis) plants in the dark[J]. Food Chemistry, 2012(135): 2268-2276.
[44] 刘晶晶,王富民,刘国峰,等. 茶树萜类香气物质代谢谱与相关基因表达谱时空变化的关系[J]. 园艺学报. 2014, 41(10): 2094-2106.
[45] Fu X M, Chen Y Y, Mei X1, et al. Regulation of formation of volatile compounds of tea(Camellia sinensis) leaves by single light wavelength[J]. Scientific Reports, 2015(11): 1-11.
[46] Lv H P, Dai W D, Tan J F, et al.Identification of the anthocyanins from the purple leaf coloured tea cultivar Zijuan(Camellia sinensis var. assamica) and characterization of their antioxidant activities[J]. Journal of Functional Foods, 2015(17): 449-458.
[47] Han Z X, Mohammad M R, Liu G F, et al.Green tea flavour determinants and their changes over manufacturing processes[J]. Food Chemistry, 2016(212): 739-748.
[48] Tsuyoshi K, Hisae K, Yumi K, et al.Characterisation of odorant compounds and their biochemical formation in green tea with a low temperature storage process[J]. Food Chemistry, 2014(148): 388-395.
[49] Youngmok K, Kevin L G, Jong D P, et al.Changes in antioxidant phytochemicals and volatile composition of Camellia sinensis by oxidation during tea fermentation[J]. Food Chemistry, 2011(129): 1331-1342.
[50] Tan J F, Dai W D, Ma Y L, et al.Study of the dynamic changes in the non-volatile chemical constituents of black tea during fermentation processing by a non-targeted metabolomics approach[J]. Food Research International, 2016(79): 106-113.
[51] Zhang L, Ning L, Yan Y C, et al.Development of the fingerprints of crude Puerh tea and ripened Puerh tea by high performance liquid chromatography[J]. Journal of Chinese Pharmaceutical Sciences, 2011(20): 352-359.
[52] Zhu Y, Lv H P, Dai W D, et al.Separation of aroma components in Xihu Longjing tea using simultaneous distillation extraction with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry[J]. Separation and Purification Technology, 2016(164): 146-154.
[53] Zhu Y, Shao C Y, Lv H P, et al.Enantiomeric and quantitative analysis of volatile terpenoids in different teas(Camellia sinensis)[J]. Journal of Chromatography A, 2017(1490): 177-190.
[54] Cui J L, Tsuyoshi K, Kojiro T, et al.Characteristic fluctuations in glycosidically bound volatiles during tea processing and identification of their unstable derivatives[J]. Journal of Agricultural and Food Chemistry, 2016, 64: 1151-1157.
[55] Lo Y H, Chen Y J, Chang C I, et al.Teaghrelins, unique acylated flavonoid tetraglycosides in Chin-shin Oolong tea, are putative oral agonists of the ghrelin receptor[J]. Journal of Agricultural and Food Chemistry, 2014, 62: 5085-5091.
[56] Dai W D, Tan J F, Lu M L, et al.Nontargeted modification-specific metabolomics investigation of glycosylated secondary metabolites in Tea (Camellia sinensis L.) based on liquid chromatography-high-resolution mass spectrometry[J]. Journal of Agricultural and Food Chemistry, 2016, 64: 6783-6790.