In this paper, a reversed-phase high performance liquid chromatography combined with precolumn derivatization with O-phthalaldehyde and ?uorescence detection was set up for the qualitatively and quantitatively analysis of seventeen kinds of free amino acids in tea. The RSD of the retention time and peak area of the 17 kinds of free amino acids were 0.02%~0.70% and 0.11%~1.15%, respectively. With validation, this method was proved to be accurate, stable and reproducible for the analysis of free amino acids in tea.
YANG Wei
,
XIAN Shu
,
LI Da-xiang
,
WAN Xiao-chun
. RP-HPLC Determination of Seventeen Free Amino Acids in Tea with O-phthalaldehyde Precolumn Derivation[J]. Journal of Tea Science, 2011
, 31(3)
: 211
-217
.
DOI: 10.13305/j.cnki.jts.2011.03.008
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