邻苯二甲醛柱前衍生反相高效液相色谱法测定茶叶中17种游离氨基酸

doi:10.13305/j.cnki.jts.2011.03.008

Abstract

Abstract: In this paper, a reversed-phase high performance liquid chromatography combined with precolumn derivatization with O-phthalaldehyde and ?uorescence detection was set up for the qualitatively and quantitatively analysis of seventeen kinds of free amino acids in tea. The RSD of the retention time and peak area of the 17 kinds of free amino acids were 0.02%~0.70% and 0.11%~1.15%, respectively. With validation, this method was proved to be accurate, stable and reproducible for the analysis of free amino acids in tea.

Key words: HPLC, pre-column derivation, O-phthaldialdehyde (OPA), free amino acid, tea

CLC Number:

TS272
O657.7⁺2

YANG Wei, XIAN Shu, LI Da-xiang, WAN Xiao-chun. RP-HPLC Determination of Seventeen Free Amino Acids in Tea with O-phthalaldehyde Precolumn Derivation[J]. Journal of Tea Science, 2011, 31(3): 211-217.

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RP-HPLC Determination of Seventeen Free Amino Acids in Tea with O-phthalaldehyde Precolumn Derivation

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